Plastik Kompozit Destek Üzerine İki Endüstriyel Şus ile Oluşturulan Biyofilm Yapılarının Tespit Edilmesi

Yıl 2016, Cilt: 2 Sayı: 2, 50 - 56, 28.12.2016

Vahap Yonten Nahit Aktas

Öz

Bu çalışmada Candida intermedia NRRL Y-8278
ve
Kluyveromces marxianus NRRL Y-8281 endüstriyel mayalarının farklı PCS destekler üzerindeki birim
alandaki koloni sayıları (CFU) geliştirilip, hesaplanmıştır. Farklı destekler
üzerindeki analiz yöntemi olarak Stripping-Sand metodu kullanılmıştır. Her bir
destek için (PCS₁, PCS₂, PCS₃, PCS₄)
hesaplamalar yapılmıştır. Kullanılan kültürlerin, plastik destekler üzerinde
biyofilm yapıları meydana getirdiği tespit edilmiştir. K. marxianus’un PCS₂ desteği üzerinde 2.9×10⁸
CFU g^-1 ve 1.6×10¹⁰
CFU mL^-1 değerlerinde koloni oluşturduğu ve  C. intermedia’dan daha iyi biofilm performansı gösterdiği gözlemlendi.
Kullanılan plastik desteklerin içerdiği polipropilen gıda, biyomedikal, hastane
ve sağlık laboratuvarları gibi birçok yerde bazı cihazların yapısında bulunmaktadır.
Ayrıca çalışmamızın sonuçları, polipropilen gibi bazı desteklerin ve
endüstriyel mayaların kullanıldığı gıda ile biomedikal teknolojileri ve
endüstriyel birçok alanda yararlı olacağı aynı zamanda bu materyallerin önem
arz ettiği kanısına varılmıştır.

 

Anahtar Kelimeler

Biyofilm, biyopolimer, CFU, PCS

The Determining of the Biofilm Form by Two Industrial Strains on Plastic Composite Supports

Öz

In
this paper, the biofilm formation of industrial yeasts Candida intermedia NRRL Y-8278 and Kluyveromces marxianus NRRL Y-8281 yeast were grown
on various plastic composite supports (PCS) to analyze colony forming unit
(CFU). The biofilm was performed using Stripping-Sand method for yeasts on the PCS
(PCS₁, PCS₂, PCS₃, PCS₄). The
biofilm formation on the yeasts was observed on the PCS. K. marxianus and C.
intermedia took place the apparent the biofilm population CFU of each yeast on plastic supports. The best
biofilm population was performed 2.9×10⁸
CFU g^-1 by K. marxianus on PCS₂ support than C. intermedia. The calculation of
biofilm in   CFU mL^-1 on
each yeast also was analyzed 1.6×10¹⁰
by K. marxianus on the PCS₂.
 K. marxianus had better the biofilm values on the PCS than C.
intermedia. The results of this work will be useful on the some areas that
this supports (polypropylene) used in such as food, biomedical and industrial
companies and to realize about structure of yeasts and polypropylene supports
in the literatures.

Anahtar Kelimeler

Biofilm, biopolymer, CFU, PCS

Kaynakça

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