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Isolation and molecular phylogeny of RFT1 gene from upland rice leaves cultivar

Year 2020, , 52 - 61, 26.08.2020
https://doi.org/10.38058/ijsl.701095

Abstract

Rice is a modern short-day plant but equally flower during non-inductive long-day condition due to the possession of second florigen, Rice Flowering Locus T1 (RFT1). RFT1 is an ortholog of Arabidopsis FT gene which triggers flowering induction under long-day (LD) condition. The gene isolation from Malaysian upland rice cv. Wai that induces flowering were reported. Nucleic acids isolation and amplification of the RFT1 were observed, then phylogenetic relationship among other 5 indica and 1 japonica rice were inferred by comparing the gene amino acids sequence data sets using multiple sequence alignment databases. The amplicon quality result indicated that the gene was fully amplified at above 0.5kb, whereas the sequence alignment revealed that the RFT1 gene was partially amplified based on the gaps identified. Among the 6 indica cultivars sequence aligned, they were almost all conserved except on only 12 amino acids. While the cladogram tree result classified the cultivars into three major groups and include Wai under clade that contains both indica and japonica cultivars. This finding concluded that the cultivars are of high evolutionary relationship and well established their molecular relationship. The cultivars relationship provides necessary information for better understanding of molecular evolution and designing scientific breeding system for generation of new rice cultivar.

Supporting Institution

UNIVERSITI TEKNOLOGI MALAYSIA

Project Number

NIL

Thanks

SPECIAL THANK TO IJSL for hiving me the opportunity

References

  • 1- DR. MOHAMMED JIBRIN NDEJIKO, DEPARTMENT OF MICROBIOLOGY, IBB-UNIVERSITY, LAPAI. NIGERI. ndejiko@gmail.com
  • 2- DR. ABDULKARIM ALI DEBA, ATBU, BAUCHI. NIGERIA. abdulkarimalideba@gmail.com
Year 2020, , 52 - 61, 26.08.2020
https://doi.org/10.38058/ijsl.701095

Abstract

Project Number

NIL

References

  • 1- DR. MOHAMMED JIBRIN NDEJIKO, DEPARTMENT OF MICROBIOLOGY, IBB-UNIVERSITY, LAPAI. NIGERI. ndejiko@gmail.com
  • 2- DR. ABDULKARIM ALI DEBA, ATBU, BAUCHI. NIGERIA. abdulkarimalideba@gmail.com
There are 2 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Research Articles
Authors

Sulaıman Mohammed 0000-0003-3094-4769

Project Number NIL
Publication Date August 26, 2020
Published in Issue Year 2020

Cite

APA Mohammed, S. (2020). Isolation and molecular phylogeny of RFT1 gene from upland rice leaves cultivar. International Journal of Science Letters, 2(2), 52-61. https://doi.org/10.38058/ijsl.701095
AMA Mohammed S. Isolation and molecular phylogeny of RFT1 gene from upland rice leaves cultivar. IJSL. August 2020;2(2):52-61. doi:10.38058/ijsl.701095
Chicago Mohammed, Sulaıman. “Isolation and Molecular Phylogeny of RFT1 Gene from Upland Rice Leaves Cultivar”. International Journal of Science Letters 2, no. 2 (August 2020): 52-61. https://doi.org/10.38058/ijsl.701095.
EndNote Mohammed S (August 1, 2020) Isolation and molecular phylogeny of RFT1 gene from upland rice leaves cultivar. International Journal of Science Letters 2 2 52–61.
IEEE S. Mohammed, “Isolation and molecular phylogeny of RFT1 gene from upland rice leaves cultivar”, IJSL, vol. 2, no. 2, pp. 52–61, 2020, doi: 10.38058/ijsl.701095.
ISNAD Mohammed, Sulaıman. “Isolation and Molecular Phylogeny of RFT1 Gene from Upland Rice Leaves Cultivar”. International Journal of Science Letters 2/2 (August 2020), 52-61. https://doi.org/10.38058/ijsl.701095.
JAMA Mohammed S. Isolation and molecular phylogeny of RFT1 gene from upland rice leaves cultivar. IJSL. 2020;2:52–61.
MLA Mohammed, Sulaıman. “Isolation and Molecular Phylogeny of RFT1 Gene from Upland Rice Leaves Cultivar”. International Journal of Science Letters, vol. 2, no. 2, 2020, pp. 52-61, doi:10.38058/ijsl.701095.
Vancouver Mohammed S. Isolation and molecular phylogeny of RFT1 gene from upland rice leaves cultivar. IJSL. 2020;2(2):52-61.