An easy and reliable method for establishment and maintenance of tissue cultures of Nicotiana tabacum cv TAPM 26

Year 2020, , 62 - 71, 26.08.2020

Yilmaz Kaya , Fahrul Zaman Huyop

https://doi.org/10.38058/ijsl.764947

Cited By: 5

Abstract

In vitro regeneration of Nicotiana tabacum was efficiently achieved using growth regulators combination supplemented into semi-solid MS medium using leaves from 1-month-old plant as explants. The growth regulators used were NAA for auxin (0.0, 1.0, 2.0, and 3.0 mgL^-1) and BAP (0.0, 0.5, 1.0, 2.5, and 5.0 mgL^-1). The effects of growth regulator combination were assessed based on a number of callus formation, shoots formation and fresh callus weight. The maximum number of callus formation was 100 % at five hormone combination, observed at the fourth week after culture. Maximum number of shoots produced per explant was 21.4 shoots at 1.0 mgL^-1 BAP + 3.0 mgL^-1 NAA, after 6 weeks of culture. The maximum callus fresh weights were obtained at 0.5 mgL^-1 BAP + 1.0 mgL^-1 NAA after 6 weeks (9.92 g). The best combination for shoots regeneration of Nicotiana tabacum was 1.0 mgL^-1 BAP + 3.0 mgL^-1 NAA. And the best combination for heaviest callus production was 0.5 mgL^-1 BAP + 1.0 mgL^-1 NAA.

Keywords

Nicotiana tabacum TAPM 26, Callus,, tissue culture

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