In vitro micropropagation of the endemic plant Nepeta baytopii Hedge & Lamond

Abstract

In this study, we sought to develop a working micropropagation protocol for the endemic plant Nepeta baytopii using biotechnological methods. After seed sterilization and germination, shoot proliferations were performed. These explants were cultured on MS medium (Murashige & Skoog, 1962) supplemented with nine different concentrations of 6-benzylaminopurine (BAP) and kinetin (KIN) applied separately (0.00, 0.0625, 0.125, 0.25, 0.5, 1, 1.5, 2, and 2.5 mg/L). The highest number of shoots was obtained on medium supplemented with 0.125 mg/L BAP. In addition, the media containing 0.125 mg/L BAP and different concentrations of α-Naphthaleneacetic acid (NAA), indole acetic acid (IAA), indole-3-butyric acid (IBA), and 2,4-dichlorophenoxyacetic acid (2,4 D) (0.00, 0.25, 0.125 and 0.0625 mg/L) were prepared separately, and the combined effects of auxin and cytokinin on shoot proliferation were investigated. The highest root formation percentage was obtained on MS medium supplemented with 0.125 mg/L BAP and 0.25 mg/L IBA. Acclimation studies have been conducted for the adaptation of rooted shoots to soil. This is the first report on the study of Nepeta baytopii’s for direct plant regeneration under conditions in vitro. This study presents an effective alternative approach for the propagation and conservation of this important endemic plant.

Keywords

• Nepeta baytopii
• Micropropagation
• Plant growth regulators

In vitro micropropagation of the endemic plant Nepeta baytopii Hedge & Lamond

Abstract

In this study, we sought to develop a working micropropagation protocol for the endemic plant Nepeta baytopii Hedge & Lamond using biotechnological methods. After seed sterilization and germination, shoot proliferations were performed. These explants were cultured on MS medium (Murashige & Skoog, 1962) supplemented with nine different concentrations of 6-benzylaminopurine (BAP) and kinetin (KIN) applied separately (0.00, 0.0625, 0.125, 0.25, 0.5, 1, 1.5, 2, and 2.5 mg/L). The highest number of shoots was obtained on medium supplemented with 0.125 mg/L BAP. In addition, the media containing 0.125 mg/L BAP and different concentrations of α-Naphthaleneacetic acid (NAA), indole acetic acid (IAA), indole-3-butyric acid (IBA), and 2,4-dichlorophenoxyacetic acid (2,4 D) (0.00, 0.25, 0.125 and 0.0625 mg/L) were prepared separately, and the combined effects of auxin and cytokinin on shoot proliferation were investigated. The highest root formation percentage was obtained on MS medium supplemented with 0.125 mg/L BAP and 0.25 mg/L IBA. Acclimation studies have been conducted for the adaptation of rooted shoots to soil. This is the first report on the study of Nepeta baytopii Hedge & Lamond’s for direct plant regeneration under conditions in vitro. This study presents an effective alternative approach for the propagation and conservation of this important endemic plant.

Keywords

• Nepeta baytopii Hedge & Lamond
• Micropropagation
• Plant growth regulators

References

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