USING IN VITRO-METHODS FOR PROPAGATION AND PRODUCING SECONDARY METABOLITES FROM EUROPEAN PENNYROYAL PLANTS (MENTHA PULEGIUM L.)

M.m. Moubarak; M. Y. Cherednıchenko

doi:10.21448/ijsm.240730

EN

USING IN VITRO-METHODS FOR PROPAGATION AND PRODUCING SECONDARY METABOLITES FROM EUROPEAN PENNYROYAL PLANTS (MENTHA PULEGIUM L.)

Abstract

European pennyroyal (Mentha pulegium L.) is the best source of essential oils and natural antioxidants. The main component of the essential oil is pulegone, which can be a precursor for the synthesis of menthol and menthofuran.The aim of this research is to develop technology in vitro cultivation of European pennyroyal (Mentha pulegium L.) to increase the essential oil content. In this research we used the various varieties and the primary explants obtained from plantlets for the induction of callus formation and somatic organogenesis in European pennyroyal plants varieties Pennyroyal and Sonia.Best response for seeds sterilization was got by using the sodium hypochlorite for 10-15 minutes.Murashige and Skoog basal medium was used for induction of morphogenesis. Using the lamina explants of Pennyroyal stem organogenesis was obtained in one modification of the ½ MS medium supplemented by 0.5 mg/l kinetin + 1 mg/l NAA - and amounted to 20%.In the variety Sonia stem organogenesis was not received on any medium modification. Petioles as explants gave no stem organogenesis on any medium modification. Nodes as explants showed stem organogenesis on all medium modifications, its effectiveness ranged between 60% and 100 %. In the variety Sonia stem organogenesis had frequency from 33 % to 100% on most medium modifications. When we used internodes explants of both varieties stem organogenesis was obtained only on MS medium supplemented by 0.5 mg/l BAP + 1 mg/l NAA, and amounted to 20%.For the induction of root organogenesis we can recommend a medium with half mineral content on MS medium supplemented by kinetin as cytokinin and NAA as auxin. Regenerates of the variety Pennyroyal developed visually better than ones of the variety Sonia. Thus for the variety Pennyroyal we can recommend MS as the mineral basic, and for the variety Sonia - ½ MS.

Keywords

European pennyroyal) Mentha pulegium L.), in vitro, antioxidants, essential oils, morphogenesis

Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

M.m. Moubarak This is me
Russian State Agrarian University – MTAA, Russia, 127550, Moscow, Timiryazevskaya St., 49, Agronomy Faculty, Department of Genetics and Biotechnology

M. Y. Cherednıchenko This is me
Russian State Agrarian University – MTAA, Russia, 127550, Moscow, Timiryazevskaya St., 49, Agronomy Faculty, Department of Genetics and Biotechnology

Publication Date

July 14, 2014

Submission Date

July 14, 2016

Acceptance Date

-

Published in Issue

Year 2014 Volume: 1 Number: 1

DOI

https://doi.org/10.21448/ijsm.240730

IZ

https://izlik.org/JA34WS96WR

Cite

RIS / Bibtex

APA

Moubarak, M., & Cherednıchenko, M. Y. (2014). USING IN VITRO-METHODS FOR PROPAGATION AND PRODUCING SECONDARY METABOLITES FROM EUROPEAN PENNYROYAL PLANTS (MENTHA PULEGIUM L.). International Journal of Secondary Metabolite, 1(1), 50. https://doi.org/10.21448/ijsm.240730

AMA

1.Moubarak M, Cherednıchenko MY. USING IN VITRO-METHODS FOR PROPAGATION AND PRODUCING SECONDARY METABOLITES FROM EUROPEAN PENNYROYAL PLANTS (MENTHA PULEGIUM L.). Int. J. Sec. Metabolite. 2014;1(1):50. doi:10.21448/ijsm.240730

Chicago

Moubarak, M.m., and M. Y. Cherednıchenko. 2014. “USING IN VITRO-METHODS FOR PROPAGATION AND PRODUCING SECONDARY METABOLITES FROM EUROPEAN PENNYROYAL PLANTS (MENTHA PULEGIUM L.)”. International Journal of Secondary Metabolite 1 (1): 50. https://doi.org/10.21448/ijsm.240730.

EndNote

Moubarak M, Cherednıchenko MY (July 1, 2014) USING IN VITRO-METHODS FOR PROPAGATION AND PRODUCING SECONDARY METABOLITES FROM EUROPEAN PENNYROYAL PLANTS (MENTHA PULEGIUM L.). International Journal of Secondary Metabolite 1 1 50.

IEEE

[1]M. Moubarak and M. Y. Cherednıchenko, “USING IN VITRO-METHODS FOR PROPAGATION AND PRODUCING SECONDARY METABOLITES FROM EUROPEAN PENNYROYAL PLANTS (MENTHA PULEGIUM L.)”, Int. J. Sec. Metabolite, vol. 1, no. 1, p. 50, July 2014, doi: 10.21448/ijsm.240730.

ISNAD

Moubarak, M.m. - Cherednıchenko, M. Y. “USING IN VITRO-METHODS FOR PROPAGATION AND PRODUCING SECONDARY METABOLITES FROM EUROPEAN PENNYROYAL PLANTS (MENTHA PULEGIUM L.)”. International Journal of Secondary Metabolite 1/1 (July 1, 2014): 50. https://doi.org/10.21448/ijsm.240730.

JAMA

1.Moubarak M, Cherednıchenko MY. USING IN VITRO-METHODS FOR PROPAGATION AND PRODUCING SECONDARY METABOLITES FROM EUROPEAN PENNYROYAL PLANTS (MENTHA PULEGIUM L.). Int. J. Sec. Metabolite. 2014;1:50.

MLA

Moubarak, M.m., and M. Y. Cherednıchenko. “USING IN VITRO-METHODS FOR PROPAGATION AND PRODUCING SECONDARY METABOLITES FROM EUROPEAN PENNYROYAL PLANTS (MENTHA PULEGIUM L.)”. International Journal of Secondary Metabolite, vol. 1, no. 1, July 2014, p. 50, doi:10.21448/ijsm.240730.

Vancouver

1.M.m. Moubarak, M. Y. Cherednıchenko. USING IN VITRO-METHODS FOR PROPAGATION AND PRODUCING SECONDARY METABOLITES FROM EUROPEAN PENNYROYAL PLANTS (MENTHA PULEGIUM L.). Int. J. Sec. Metabolite. 2014 Jul. 1;1(1):50. doi:10.21448/ijsm.240730