In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker

Abstract

Muscari aucheri (Boiss.) Baker bears beautiful and attractive purplish blue flowers bloom between May and June; it grows stony slopes, mountain pastures an altitude of 1000-3000 meters. The natural populations of Muscari aucheri, is seriously affected by increased environmental pollution, urbanization and grazing. Therefore, this study aimed to accelerate multiplication by devising a strategy for an efficient in vitro bulblet regeneration system of Muscari aucheri using stem explants on MS medium containing 1, 3, 5 mg/l TDZ plus 0, 0.1, 0.2, 0.4 mg/l NAA (12 combinations). The stem explants induced direct bulblet regeneration on explants. Maximum mean number of bulblets per explant was noted on MS medium containing 3 mg/l TDZ + 0.4 mg/l NAA and maximum mean number of bulb diameter was noted on MS medium containing 5 mg/l TDZ + 0.2 mg/l NAA. The regenerated bulblets were isolated from stem explants and cultured on MS medium containing 40 g/l sucrose; where they gained diameter and rooted

Keywords

• Muscari aucheri
• in vitro
• bulb regeneration

In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker

Abstract

Muscari aucheri (Boiss.) Baker bears beautiful and attractive purplish blue flowers bloom between May and June; it grows stony slopes, mountain pastures an altitude of 1000-3000 meters. The natural populations of Muscari aucheri, is seriously affected by increased environmental pollution, urbanization and grazing. Therefore, this study aimed to accelerate multiplication by devising a strategy for an efficient in vitro bulblet regeneration system of Muscari aucheri using stem explants on MS medium containing 1, 3, 5 mg/l TDZ plus 0, 0.1, 0.2, 0.4 mg/l NAA (12 combinations). The stem explants induced direct bulblet regeneration on explants. Maximum mean number of bulblets per explant was noted on MS medium containing 3 mg/l TDZ + 0.4 mg/l NAA and maximum mean number of bulb diameter was noted on MS medium containing 5 mg/l TDZ + 0.2 mg/l NAA. The regenerated bulblets were isolated from stem explants and cultured on MS medium containing 40 g/l sucrose; where they gained diameter and rooted

Keywords

• Muscari aucheri
• in vitro
• bulb regeneration

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