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In vitro Propagation Techniques for Some Geophyte Ornamental Plants with High Economic Value

Year 2015, Volume 2, Issue 1, 27 - 49, 14.01.2015

Abstract

Propagation of some ornamental plants has increased tremendously due to the demand for them as cut flowers, in addition to their usage for interior and exterior landscaping purposes. Geophytes (bulbous-tuberous) are the most preferred group among the ornamental plants due to their aesthetic features, suitability to be cut flowers and their fragrance. These plants are highly propagated and consumed. The geophyte species which are highly profitable, globally traded and constituting 90% of the flower bulb market are Tulipa (tulip), Lilium (lily), Narcissus (daffodil), Gladiolus (gladioli), Iris (iris) and Hyacinthus (hyacinth). In vitro propagation techniques, which provide disease-free mass production options, have started to be used increasingly to fulfil the demand for these species in the market. In this study, the results of in vitro propagation studies for some economically valuable tulip, lily, daffodil, gladiolus, iris and hyacinth species are provided.

References

  • Zhen-guang, C., 1982.
  • Nutrient medium+active charcoal
  • active carbon in basic medium
  • Pifang, Z. et al., 1985b. N. chinensis
  • The white compact callus was initiated from scale segments with
  • the basal plate on MS+1 mg l-1 BA and 0.1 mg l-1 2,4-D.
  • Adventitious shoots were produced from callus cultures by
  • transferring them on MS or with 1 mg l-1 BA or containing 0.1-0.5
  • NAA. Most of bulblets developed leaves and roots ½ MS+0.01-0.1 mg l NAA or without any growth substance, particularly with 0.03 mg l-1 NAA.
  • Hengsen, G. and Cuihua, G., 1987. N. chinensis
  • MS+different doses of PGR
  • increased adventitious shoots on MS+0.1 mg l-1 NAA.
  • Hengsen, G. et al., 1987. N. chinensis MS+0-5 mg l-1 BA, 0-1 mg l induced bulbils. The percentage of induction reached 70%.
  • Yimin, H. and Guoning, Q., 1991. N. chinensis MS+NAA and 6-BA
  • The differentiation rate of puff callus induced by low concentration
  • of NAA (0. 5 mg l)+6 BA (1-8 mg l-1) is low.
  • Weilian, H. et al., 1993. Narcissus c.v.s
  • “St. Keverne” and “hawera” MS+different doses of PGR
  • BA in single leaf cultures than in shoot clump cultures. NAA
  • stimulated bulbil formation on MS+176 mM sucrose for both
  • cultivars. “St Keverne” showed good bulbil development with 0.54
  • μM NAA, 5.4 μM IAA and 5.4 μM lBA and “Hawera” responded only to 27 μM IAA.
  • Staikidou, I. et al., 1994.
  • N. bulbocodium Twin scales PGR
  • mg l-1) resulted in shoot initiation and leaf development. Tiny bulbs
  • were obtained with MS+BAP+IBA for a long period (70 days). The
  • final size of the bulbs was not increased by the presence of NAA
  • butlincrease but a better root system was developed by it.
  • Santos, J. et al., 1998.
  • N. pseudonarcissus Leaves, bulbs and c.v.s
  • harvest” and “St. Keverne” MS+different doses of A range of 2,4-D and BAP concentrations started embryogenesis. 5 μM 2,4-D and 0.5 μM or 5 μM BAP was more efficieny on somatic
  • embryogenesis (SEs) than other combinations. SEs were produced
  • on scape explants earlier. SEs converted to plantlets with 4.9 μM IBA.
  • Sage, D.O. et al., 2000. N. chinensis PGR
  • mg l-1 2,4-D. The calli could differentiate on the media added BA
  • and NAA. But the root differentiation rate could be increased, and
  • the differentiation rate of buds decreased with increasing of NAA
  • concentration in the media. The buds could grow up to small plants
  • on the MS+BA and NAA. Yu, W., 2001.
  • Narcissus c.v. “pink Young leaves charm”
  • MS+different doses of PGR
  • medium for differentiation of rosette bud. For proliferation: MS+1.5
  • BA +1.0 mg lNAA and for induction of roots: ½ MS+0.2 mg l-1 6-BA+0.5 mg lNAA.
  • Zhu, H. et al., 2007. Narcissus “fortissimo” ½ MS+different doses of
  • The root of bulblets grew strongly on ½ MS+0.1 mg l-1 NAA or 0.1
  • mg l-1 IBA. The root was shorter and slimmer on ½ MS+0.05 mg l
  • IBA. The bulblets grew faster on ½ MS+1.0 mg l-1 NAA or 0.5 mg
  • l-1 IBA. ½ MS+0.1 mg l-1 NAA or 0.1 mg l-1 IBA had the significant
  • promotion on the rooting induction of tissue culture seedlings in narcissus.
  • Cui, W., 2008. N. suzhou Double scales
  • The best medium was MS+3.2 mg l-1 6-BA+.02 mg l-1 NAA for
  • inducement. The average of inducing small bulbs was 4-5 and the
  • increment rate was up to 322.22%.
  • Jiang, L. et al., 2010. Narcissus “delibes”
  • MS+different doses of PGR
  • 0 g·l-1sucrose; for bulbil formation: MS+4.0 mg l-1 6-BA, 0.2 mg
  • NAA, 2.0 mg lactivated charcoal and sucrose 60.0 g·l or MS+
  • 0 mg l-16-BA, 1.0 mg l-12,4-D, 2.0 mg l-1 activated charcoal and
  • 0 g·l-1sucrose; for roots: MS +1.0 mg l-1 6-BA, 0.5 mg l-1 2,4-D,
  • 5 mg l-1 NAA, 2.0 mg l-1 activated charcoal and 30.0 g·l-1 sucrose.
  • LV., X. et. al., 2010. Narcissus “arkle”
  • different parts of bulbs
  • Twin-scale with basal plate was more suitable explant was
  • described. The more appropriate medium for primary culture was
  • MS+3.0 mg l-1 6-BA+0.5 mg l-1 NAA+0.2 mg l-1 IBA. The
  • proliferation medium was MS+1.5 mg l-1 6-BA+0.3 mg l-1 NAA, its
  • induction rate was 668%.The rooting rate of the bulblets was 80%
  • on ½ MS+0.1 mg l-1 NAA+1 g·l-1 activated carbon. The
  • combination of 6-BA and NAA had favourable effects on induction
  • and multiplication of bulblets. ½ MS+ NAA and AC was beneficial to rooting.
  • Sun, X. et. al., 2010a.
  • MS+different doses of PGR
  • the capacity to induce colorless embryogenic calli. Production of
  • shoot buds was stimulated with the moderate concentration of 2,4
  • D (0.5-1 mg l-1). It was found that different calli induction and
  • organogenesis were dependent on the auxin type and their
  • concentrations in the medium.
  • Fang, Q. et al., 2013. Plant Explant Types
  • Culture Medium Results
  • Hyacinthus sp. H. orientalis Bulb segments
  • 300 bulblets which are 3-4 cm long and 0.5 cm wide were
  • obtained by basal scale segments. References
  • Pierik, R.L.M. and Post, A.J.M., 1974. H. orientalis “lady derby” NAA+BA bulblet initiation It was found that NAA was the only necessary hormone for callus formation and root differentiation.
  • Saniewski, M. et al., 1974. Hyacinthus subsp. Bulb scales, basal
  • plates, leaf, ovary and stem of PGR Hussey, G., 1975.
  • Hyacinthus subsp. Peduncle,
  • pedicel and flower bud of PGR
  • NAA+BA added NAA dose is less than that of BA, the bulblets are
  • differentiated from explants.
  • Jinyu, D. and Hong, H., 1983. Hyacinthus subsp. Bulb scales and leaflet explants
  • medium+different doses of IBA and BA
  • formed, and the fresh weight of their bulblets were affected.
  • Van Aartrijk, J. et al., 1986. H. orientalis Bulb scales NAA or BA
  • and 0.54–5.4 μM NAA, but their growth was slow. Based on the
  • results, a mass propagation scheme for H. orientalis using shake
  • culture had been established.
  • Takayama, S. et al., 1991. H. orientalis Leaves Nutrient
  • medium+different doses
  • of PGR+different types of sugars shoots and tubers.
  • Bach, A. et al., 1992. Hyacinthus orientalis
  • MS+different doses of NAA and BA
  • callus and shoot bud. MS+2 mg l-1 BA+2 mg l-1 NAA was found to
  • be suitable for callus and shoot bud. But without hormones medium
  • was suitable root induction.
  • Yanbo, L. et al., 1998. H. “white pearl” Nutrient medium
  • Continuous differentiation of tepals was successively induced. In
  • days, each flower bud differentiated an average of more than 70
  • tepals. It was found that the first whorled organ of the flower bud
  • was perianth which consisted of perianth tube and tepals grown at
  • the top of the perianth tube, which is the same as the flower bud of
  • the wild type in H. orentalis.
  • Wenliang, L. et al.,1999. H. orientalis “delft blue”
  • MS+ different types sand doses of sugars
  • medium doses sugars. The highest proliferation rate of adventitious
  • buds were obtained by 360 mM glucose.
  • Bach, A. and Swiderski, A., 2000. Hyacinthus orientalis pedicel peduncle μM per inflorescence explant.
  • Ziv, M. and Lilien-Kipnis, H., 2000. H. orientalis “delft blue” from immature leaves
  • Bulb formation was promoted by adding of ABA to the medium
  • although adding of fluridone inhibited it.
  • Li, H. et. al., 2002. H. orientalis “carnegie”
  • Modified Heller (1953) of IAA+IBA
  • IBA. The highest bulblet height was 0.9 cm on medium+1.5 mg l-1
  • IAA while bulblet diameter was 0.8 cm in medium+1.5 mg l-1 IAA
  • or medium+1.5 mg l-1 IBA.
  • Y.B. et al., 2002. H. orientalis “blue jacket”
  • MS+2 ppm BAP+0.25 ppm NAA were obtained.
  • Çığ, A. et al., 2006. H. orientalis
  • Bulb scale, leaf primordia immature inflorescence
  • The scale explant was found to be appropriate for callus. Calli were
  • obtained MS+0.5 mg l-1 IBA or MS+1 mg l-1 IBA. In indirect
  • organogenesis and MS+3 mg l-1 BAP+0.3 mg l-1 IBA, the best
  • results of producing bulblet (3.06 bulblets) were achieved.
  • Salehzadeh, S. et al., 2008. H. orientalis “gipsy queen” tender leaves of PGR
  • NAA. The appropriate medium for tender leafs was: MS+3.0 mg l-1
  • BA+0.2 mg l-1 NAA, and its effect was better than that of scale
  • segments. The best medium on subculture multiplication was
  • MS+2.0 mg l-1 6-BA+0.5 mg l-1 NAA+0.2 mg l-1 Kinetin. Root
  • induction for the bulblets was beter on ½ MS+0.2 mg lNAA.
  • Sun, X. et al., 2010b.
  • Bo J., Jifang W., Chunlang J., Zhengxiu D., (1984). A preliminary research on the propagation
  • of gladiola tissue culture. Acta Horticulturae Sinica, 1984-02. Boltenkov E.V., Labetskaya N.V., Lauve L.S., Zhuravlev Y.N., (2000). Remove from marked
  • records introduction of Iris oxypetala Bunge into in vitro culture. Rastitel'nye Resursy, 36 (1): 67-70. Buschman J.C.M., (2005). Globalisation flower-flower bulbs-bulb flowers. Acta Hort. 673: 27- 33. Cai-hua L., Jin-ping F., Shu-fang G., Dai-di C., (2012). Study on mutant induction
  • of Gladiolus by in vitro culture of petals. Journal of Northeast Agricultural University,19 (3): 38-42. Chu C.C., Wang C.C., Sun C.S., (1975). Establishment of an efficient medium for anther culture
  • of rice through comparative experiments on the nitrogen sources. Sci. Sin.18: 659-668. Cui W., (2008). Effects of hormone on the rooting of tissue culture seedlings
  • in Narcissus. Journal of Anhui Agricultural Sciences, 2008-18. Custers J.B.M., Eikelboom, W., Bergervoet J.H.W., Eijk J.P., (1992). In ovulo embryo culture
  • of tulip (Tulipa L.); effects of culture conditions on seedling and bulblet
  • formation. Scientia Horticulturae, 51 (1–2): 111-122. Çığ A., Yaşar F., Üzal Ö., Türkoğlu N., Yılmaz H., (2006). Sümbül soğanın doku kültüründe
  • çoğaltılması. III. Ulusal Süs Bitkileri Kongresi. 8-10 Kasım. 2006. İzmir. Dantu P.K., Bhojwani S.S., (1995). In vitro corm formation and field evaluation of corm
  • derived plants of Gladiolus. Scientia Horticulturae, 61: 115-129. De Hertogh A., Schepeen J.M., Kamenetsky R., Le Nard M., Okubo H., (2012). The
  • Micropropagation of Ixia viridifolia and
  • a Gladiolus × Homoglossum hybrid. Scientia Horticulturae, 29 (1-2): 181-189. Takayama S., Amo T., Fukano M., (1991). Rapid clonal propagation of Hyacinthus
  • orientalis bulbs by shake culture. Scientia Horticulturae, 45 (3-4): 315-321. Tang D., Wang Y., Xu J., Li W., Tian G., Tang K., (2009). Adventitious shoot induction and
  • plant regeneration from leaf explants of Lilium longiflorum Thunb. Propagation of
  • Ornamental Plants, 9 (2): 84-89. Tıpırdamaz R., (2003). Rooting and acclimatization of in vitro micropropagated snowdrop
  • Symposium on Flower Bulbs. Van Der Linde P.C.G., Blom-Barnhoorn G.J., Van Aartrijk J., (1986). Towards "in vitro"
  • propagation of bulbous iris. ISHS Acta Horticulturae 177: IV International Symposium
  • on Flower Bulbs. Wang Y., Tang R., Wang J., Wei M., (2003). The influence of the growth regulators on the
  • organogenesis in tissue culture of the Gladiolus stem sections. Journal of Qinghai
  • University, 2003-03. Weilian H., Mingshan S., Jie G., Muchuan C., (1993). Eleetron microscopic observation on the
  • tissue culture and the dedifferentiation of Narcissus tazetta var. Chinensis flower
  • peduncle. Journal of Xiamen University (Natural Science), 1993-S1. Weiyan Z., Zhiran S., (1986). Flower bud and dormant but culture and piants redifferentiation
  • of Gladiolus hybridus in vitro. Journal of China Agricultural University, 1986-02. Wenliang L., Shunong B., Xiansheng Z., (1999). Induction of continuous tepal differentiation
  • from in vitro regenerated flower buds of Hyacinthus orientalis. Acta Botanica Sinica, 41 (9): 921-926. Yanbo L., Shanna C., Yan L., Zhihao H., Tianxin, L., (1998). Tissue culture from ovary
  • of Hyacinthus orientalis L. Journal of Yunnan University (Natural Sciences), 1998-05. Yang W., Zhang O., Pan H., Sun M., (2010). In vitro regeneration of Lilium tsingtauense Gilg. and analysis of genetic variability in
  • micropropagated plants
  • using RAPD and ISSR techniques. Propagation of Ornamental Plants, 10 (2): 59-66. Yi Y.B., Lee K.S, Chung C.H., (2002). Protein variation and efficient in vitro culture of scale
  • segments from Hyacinthus orientalis. Scientia Horticulturae, 92: 367-374. Yimin H., Guoning Q., (1991). Study on the propagation technique in vitro for new varieties
  • Huazhong Agricultural University, 2008-01. Zhang M., Jia G., (2014). The effects of sucrose concentration and light condition on lily’s
  • bulblet-in-tube production and inclusion content. Pak. J. Bot., 46 (1): 307-315. Zhao C., (2012). Study on the rooting of tissue culture seedlings fromt new varieties
  • of Iridaceae. Journal of Anhui Agricultural Sciences, 2012-27. Zhao Y., (2005). Effect of SA on formation and growth of micro-bulb of Tulipa
  • gesneriana. Journal of Anhui Agricultural Sciences, 2005-09. Zheng Y., Zou C., Cao Y., (2007). Effect of PP-(333) on growth proliferation and rooting
  • of Gladiolus hybridus in tissue culture. Liaoning Agricultural Sciences, 2007-02. Zhen-guang C., (1982). A preliminary investigation on the propagation of Narcissus
  • tazetta var. Chinensis Roem by tissue culture. Journal of Fujian Agriculture and Forestry
  • University (Natural Science Edition), 1982-01. Zhou
  • Y., Zhang J., Chen M., Gu J., (2013). Development of plant regeneration system via
  • somatic embryogenesis from roots of Lilium hybrid cultivars. Propagation of Ornamental
  • Plants, 13 (3): 130-137. Zhu H., Zheng S., Li W., Lu X., (2007). Study on the tissue culture and rapid propagation
  • of Narcissus L. cv. Pink Charm. Journal of Jiangsu Forestry Science &
  • Technology, 2007-01. Ziv M., Lilien-Kipnis H., (2000). Bud regeneration from inflorescence explants for rapid
  • propagation of geophytes in vitro. Plant Cell Reports 19: 845-850.

In vitro Propagation Techniques for Some Geophyte Ornamental Plants with High Economic Value

Year 2015, Volume 2, Issue 1, 27 - 49, 14.01.2015

Abstract

Propagation of some ornamental plants has increased tremendously due to the demand for them as cut flowers, in addition to their usage for interior and exterior landscaping purposes. Geophytes (bulbous-tuberous) are the most preferred group among the ornamental plants due to their aesthetic features, suitability to be cut flowers and their fragrance. These plants are highly propagated and consumed. The geophyte species which are highly profitable, globally traded and constituting 90% of the flower bulb market are Tulipa (tulip), Lilium (lily), Narcissus (daffodil), Gladiolus (gladioli), Iris (iris) and Hyacinthus (hyacinth). In vitro propagation techniques, which provide disease-free mass production options, have started to be used increasingly to fulfil the demand for these species in the market. In this study, the results of in vitro propagation studies for some economically valuable tulip, lily, daffodil, gladiolus, iris and hyacinth species are provided.

References

  • Zhen-guang, C., 1982.
  • Nutrient medium+active charcoal
  • active carbon in basic medium
  • Pifang, Z. et al., 1985b. N. chinensis
  • The white compact callus was initiated from scale segments with
  • the basal plate on MS+1 mg l-1 BA and 0.1 mg l-1 2,4-D.
  • Adventitious shoots were produced from callus cultures by
  • transferring them on MS or with 1 mg l-1 BA or containing 0.1-0.5
  • NAA. Most of bulblets developed leaves and roots ½ MS+0.01-0.1 mg l NAA or without any growth substance, particularly with 0.03 mg l-1 NAA.
  • Hengsen, G. and Cuihua, G., 1987. N. chinensis
  • MS+different doses of PGR
  • increased adventitious shoots on MS+0.1 mg l-1 NAA.
  • Hengsen, G. et al., 1987. N. chinensis MS+0-5 mg l-1 BA, 0-1 mg l induced bulbils. The percentage of induction reached 70%.
  • Yimin, H. and Guoning, Q., 1991. N. chinensis MS+NAA and 6-BA
  • The differentiation rate of puff callus induced by low concentration
  • of NAA (0. 5 mg l)+6 BA (1-8 mg l-1) is low.
  • Weilian, H. et al., 1993. Narcissus c.v.s
  • “St. Keverne” and “hawera” MS+different doses of PGR
  • BA in single leaf cultures than in shoot clump cultures. NAA
  • stimulated bulbil formation on MS+176 mM sucrose for both
  • cultivars. “St Keverne” showed good bulbil development with 0.54
  • μM NAA, 5.4 μM IAA and 5.4 μM lBA and “Hawera” responded only to 27 μM IAA.
  • Staikidou, I. et al., 1994.
  • N. bulbocodium Twin scales PGR
  • mg l-1) resulted in shoot initiation and leaf development. Tiny bulbs
  • were obtained with MS+BAP+IBA for a long period (70 days). The
  • final size of the bulbs was not increased by the presence of NAA
  • butlincrease but a better root system was developed by it.
  • Santos, J. et al., 1998.
  • N. pseudonarcissus Leaves, bulbs and c.v.s
  • harvest” and “St. Keverne” MS+different doses of A range of 2,4-D and BAP concentrations started embryogenesis. 5 μM 2,4-D and 0.5 μM or 5 μM BAP was more efficieny on somatic
  • embryogenesis (SEs) than other combinations. SEs were produced
  • on scape explants earlier. SEs converted to plantlets with 4.9 μM IBA.
  • Sage, D.O. et al., 2000. N. chinensis PGR
  • mg l-1 2,4-D. The calli could differentiate on the media added BA
  • and NAA. But the root differentiation rate could be increased, and
  • the differentiation rate of buds decreased with increasing of NAA
  • concentration in the media. The buds could grow up to small plants
  • on the MS+BA and NAA. Yu, W., 2001.
  • Narcissus c.v. “pink Young leaves charm”
  • MS+different doses of PGR
  • medium for differentiation of rosette bud. For proliferation: MS+1.5
  • BA +1.0 mg lNAA and for induction of roots: ½ MS+0.2 mg l-1 6-BA+0.5 mg lNAA.
  • Zhu, H. et al., 2007. Narcissus “fortissimo” ½ MS+different doses of
  • The root of bulblets grew strongly on ½ MS+0.1 mg l-1 NAA or 0.1
  • mg l-1 IBA. The root was shorter and slimmer on ½ MS+0.05 mg l
  • IBA. The bulblets grew faster on ½ MS+1.0 mg l-1 NAA or 0.5 mg
  • l-1 IBA. ½ MS+0.1 mg l-1 NAA or 0.1 mg l-1 IBA had the significant
  • promotion on the rooting induction of tissue culture seedlings in narcissus.
  • Cui, W., 2008. N. suzhou Double scales
  • The best medium was MS+3.2 mg l-1 6-BA+.02 mg l-1 NAA for
  • inducement. The average of inducing small bulbs was 4-5 and the
  • increment rate was up to 322.22%.
  • Jiang, L. et al., 2010. Narcissus “delibes”
  • MS+different doses of PGR
  • 0 g·l-1sucrose; for bulbil formation: MS+4.0 mg l-1 6-BA, 0.2 mg
  • NAA, 2.0 mg lactivated charcoal and sucrose 60.0 g·l or MS+
  • 0 mg l-16-BA, 1.0 mg l-12,4-D, 2.0 mg l-1 activated charcoal and
  • 0 g·l-1sucrose; for roots: MS +1.0 mg l-1 6-BA, 0.5 mg l-1 2,4-D,
  • 5 mg l-1 NAA, 2.0 mg l-1 activated charcoal and 30.0 g·l-1 sucrose.
  • LV., X. et. al., 2010. Narcissus “arkle”
  • different parts of bulbs
  • Twin-scale with basal plate was more suitable explant was
  • described. The more appropriate medium for primary culture was
  • MS+3.0 mg l-1 6-BA+0.5 mg l-1 NAA+0.2 mg l-1 IBA. The
  • proliferation medium was MS+1.5 mg l-1 6-BA+0.3 mg l-1 NAA, its
  • induction rate was 668%.The rooting rate of the bulblets was 80%
  • on ½ MS+0.1 mg l-1 NAA+1 g·l-1 activated carbon. The
  • combination of 6-BA and NAA had favourable effects on induction
  • and multiplication of bulblets. ½ MS+ NAA and AC was beneficial to rooting.
  • Sun, X. et. al., 2010a.
  • MS+different doses of PGR
  • the capacity to induce colorless embryogenic calli. Production of
  • shoot buds was stimulated with the moderate concentration of 2,4
  • D (0.5-1 mg l-1). It was found that different calli induction and
  • organogenesis were dependent on the auxin type and their
  • concentrations in the medium.
  • Fang, Q. et al., 2013. Plant Explant Types
  • Culture Medium Results
  • Hyacinthus sp. H. orientalis Bulb segments
  • 300 bulblets which are 3-4 cm long and 0.5 cm wide were
  • obtained by basal scale segments. References
  • Pierik, R.L.M. and Post, A.J.M., 1974. H. orientalis “lady derby” NAA+BA bulblet initiation It was found that NAA was the only necessary hormone for callus formation and root differentiation.
  • Saniewski, M. et al., 1974. Hyacinthus subsp. Bulb scales, basal
  • plates, leaf, ovary and stem of PGR Hussey, G., 1975.
  • Hyacinthus subsp. Peduncle,
  • pedicel and flower bud of PGR
  • NAA+BA added NAA dose is less than that of BA, the bulblets are
  • differentiated from explants.
  • Jinyu, D. and Hong, H., 1983. Hyacinthus subsp. Bulb scales and leaflet explants
  • medium+different doses of IBA and BA
  • formed, and the fresh weight of their bulblets were affected.
  • Van Aartrijk, J. et al., 1986. H. orientalis Bulb scales NAA or BA
  • and 0.54–5.4 μM NAA, but their growth was slow. Based on the
  • results, a mass propagation scheme for H. orientalis using shake
  • culture had been established.
  • Takayama, S. et al., 1991. H. orientalis Leaves Nutrient
  • medium+different doses
  • of PGR+different types of sugars shoots and tubers.
  • Bach, A. et al., 1992. Hyacinthus orientalis
  • MS+different doses of NAA and BA
  • callus and shoot bud. MS+2 mg l-1 BA+2 mg l-1 NAA was found to
  • be suitable for callus and shoot bud. But without hormones medium
  • was suitable root induction.
  • Yanbo, L. et al., 1998. H. “white pearl” Nutrient medium
  • Continuous differentiation of tepals was successively induced. In
  • days, each flower bud differentiated an average of more than 70
  • tepals. It was found that the first whorled organ of the flower bud
  • was perianth which consisted of perianth tube and tepals grown at
  • the top of the perianth tube, which is the same as the flower bud of
  • the wild type in H. orentalis.
  • Wenliang, L. et al.,1999. H. orientalis “delft blue”
  • MS+ different types sand doses of sugars
  • medium doses sugars. The highest proliferation rate of adventitious
  • buds were obtained by 360 mM glucose.
  • Bach, A. and Swiderski, A., 2000. Hyacinthus orientalis pedicel peduncle μM per inflorescence explant.
  • Ziv, M. and Lilien-Kipnis, H., 2000. H. orientalis “delft blue” from immature leaves
  • Bulb formation was promoted by adding of ABA to the medium
  • although adding of fluridone inhibited it.
  • Li, H. et. al., 2002. H. orientalis “carnegie”
  • Modified Heller (1953) of IAA+IBA
  • IBA. The highest bulblet height was 0.9 cm on medium+1.5 mg l-1
  • IAA while bulblet diameter was 0.8 cm in medium+1.5 mg l-1 IAA
  • or medium+1.5 mg l-1 IBA.
  • Y.B. et al., 2002. H. orientalis “blue jacket”
  • MS+2 ppm BAP+0.25 ppm NAA were obtained.
  • Çığ, A. et al., 2006. H. orientalis
  • Bulb scale, leaf primordia immature inflorescence
  • The scale explant was found to be appropriate for callus. Calli were
  • obtained MS+0.5 mg l-1 IBA or MS+1 mg l-1 IBA. In indirect
  • organogenesis and MS+3 mg l-1 BAP+0.3 mg l-1 IBA, the best
  • results of producing bulblet (3.06 bulblets) were achieved.
  • Salehzadeh, S. et al., 2008. H. orientalis “gipsy queen” tender leaves of PGR
  • NAA. The appropriate medium for tender leafs was: MS+3.0 mg l-1
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Details

Primary Language English
Subjects Biology
Other ID JA37RM38TB
Journal Section Articles
Authors

Arzu ÇIĞ This is me
Siirt University, Faculty of Agriculture, Department of Horticulture, 56100, Siirt-Turkey


Gülçinay BAŞDOĞAN This is me
YUZUNCU YIL UNIVERSITY, FACULTY OF AGRICULTURE, DEPARTMENT OF LANDSCAPE ARCHITECTURE

Publication Date January 14, 2015
Published in Issue Year 2015, Volume 2, Issue 1

Cite

APA Çığ, A. & Başdoğan, G. (2015). In vitro Propagation Techniques for Some Geophyte Ornamental Plants with High Economic Value . International Journal of Secondary Metabolite , 2 (1) , 27-49 . Retrieved from https://dergipark.org.tr/en/pub/ijsm/issue/22525/240708

International Journal of Secondary Metabolite (IJSM)

ISSN-e: 2148-6905