Effect of Phytohormones of Kinetin and Epibrassinolide on Content and Intracellular Localization of Glucosides and Free Amino Acids in Pea Plants Cells (Pisum sativum L.)

Year 2018, Volume: 5 Issue: 2, 156 - 162, 05.07.2018

Antonina N. Ershova Natalia Vinokurova

https://doi.org/10.21448/ijsm.422077

Abstract

During injection of kinetin and epibrassinolide in pea seedlings (Pisum sativum L.) an increase of content of specific for this compound isosuccinimide-β-glucoside (IS-glucoside) for 15-30%, ethyl-β-glucoside for 40-80%, GABA free amino acids, glutamate and alanine only under kinetin for 15-20% was observed. The field experiments showed an increase of aglycone content but the amount of IS-glucoside decreased at first and then increased 14 days after plant treatment with phytohormones. It was shown that effect of phytohormones on content of IS-glucosides and free amino acids was dependent on action time and age of plant. By using membrane trophic compound of DMSO it was discovered that in pea seedlings cells the vacuolar fund of IS-glucoside was 70% of initial and aglycone with γ-aminobutyric acid as its precursor were localized in cytoplasm. Aspartate and glutamate were almost equally distributed between cytoplasmic and vacuolar cell funds. It is suggested that reactions of pyrrolidone structure of IS-glucoside aglycone formation and synthesis of the glucoside were processed in cytoplasm. Further IS-glucoside was transferred and stored in vacuoles of pea seedlings cells by contrast with ethyl-β-glucoside mainly localized in cytoplasm.

Keywords

GABA, Ethyl-β-glucoside, Isosuccinimide-β-glucoside, Vacuole

Effect of Phytohormones of Kinetin and Epibrassinolide on Content and Intracellular Localization of Glucosides and Free Amino Acids in Pea Plants Cells (Pisum sativum L.)

Abstract

During
injection of kinetin and epibrassinolide in pea seedlings (Pisum sativum L.) an increase of content of specific for this
compound isosuccinimide-β-glucoside (IS-glucoside) for 15-30%,
ethyl-β-glucoside for 40-80%, GABA free amino acids, glutamate and alanine only
under kinetin for 15-20% was observed. The field experiments showed an increase
of aglycone content but the amount of IS-glucoside decreased at first and then
increased 14 days after plant treatment with phytohormones. It was shown that
effect of phytohormones on content of IS-glucosides and free amino acids was
dependent on action time and age of plant. By using membrane trophic compound
of DMSO it was discovered that in pea seedlings cells the vacuolar fund of
IS-glucoside was 70% of initial and aglycone with γ-aminobutyric acid as its
precursor were localized in cytoplasm. Aspartate and glutamate were almost
equally distributed between cytoplasmic and vacuolar cell funds. It is
suggested that reactions of pyrrolidone structure of IS-glucoside aglycone
formation and synthesis of the glucoside were processed in cytoplasm. Further
IS-glucoside was transferred and stored in vacuoles of pea seedlings cells by
contrast with ethyl-β-glucoside mainly localized in cytoplasm.

Keywords

GABA, Ethyl-β-glucoside, Isosuccinimide-β-glucoside, Vacuole

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