Effects of Nitrate toxicity on free Proline accumulation, chlorophyll degradation and photosynthetic efficiency in Chlorella vulgaris Beyerinck [Beijerinck]

Year 2019, Volume: 6 Issue: 1, 10 - 19, 16.03.2019

İnci Tüney Kızılkaya , Dilek Unal

https://doi.org/10.21448/ijsm.471036

Cited By: 7

Abstract

The effects of high nitrate concentrations on alterations in maximal photochemical efficiency of PSII ratio (Fv/Fm), chlorophyll content, chlorophyll degradation, growth rate and proline accumulation in Chlorella vulgaris Beyerinck [Beijerinck] were investigated in this study. The Fv/Fm ratio was observed in response to the increased nitrate concentration. The Fv/Fm ratio decreased in C. vulgaris following 44 h in 12.99 mM NaNO3-enriched media. Experimental results showed that, growth rates and chlorophyll content declined by increasing nitrate concentration. The decrease in the ratio of chlorophyll a/b with enrichment of high nitrate concentration (6.5 mM and 12.99 mM NaNO3) was also caused by a decrease in chlorophyll a and an increase in chlorophyll b concentration in C. vulgaris cultures. The results showed that 6.5 and 12.99 mM nitrate concentration increased proline content in treated cells, which suggests that nitrate stress lead to proline accumulation in C. vulgaris.

Keywords

Chlorella vulgaris, Chlorophyll degradation, Nitrate stress, Proline, Fv/Fm

Effects of Nitrate toxicity on free Proline accumulation, chlorophyll degradation and photosynthetic efficiency in Chlorella vulgaris Beyerinck [Beijerinck]

Abstract

The
effects of high nitrate concentrations on alterations in maximal photochemical
efficiency of PSII ratio (Fv/Fm), chlorophyll content, chlorophyll degradation,
growth rate and proline accumulation in Chlorella vulgaris Beyerinck
[Beijerinck] were investigated in this study. The Fv/Fm ratio was
observed in response to the increased nitrate concentration. The Fv/Fm ratio
decreased in C. vulgaris following 44 h in 12.99 mM NaNO₃-enriched
media. Experimental results showed that, growth rates and chlorophyll content
declined by increasing nitrate concentration. The decrease in the ratio of
chlorophyll a/b with enrichment of high nitrate concentration (6.5 mM and 12.99
mM NaNO₃) was also caused by a decrease in chlorophyll a and an
increase in chlorophyll b concentration in C. vulgaris cultures. The
results showed that 6.5 and 12.99 mM nitrate concentration increased proline
content in treated cells, which suggests that nitrate stress lead to proline
accumulation in C. vulgaris.

Keywords

C. vulgaris, Chlorophyll degradation, nitrate stress, proline, Fv/Fm

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