The Role of p16 Expression in Recurrence and Survival of Laryngeal Squamous Cell Carcinomas

Abstract

Objective: The most common histological type in laryngeal carcinomas is squamous cell carcinoma. In recent years, it has been reported that human papilloma virus may also be effective among the main causative factors. Immunohistochemically, p16 protein is evaluated in identifying HPV infection. The aim of this study was to evaluate the prognostic role of p16 expression on long-term overall survival, disease-free survival, and disease-specific ‘progression-free’ survival in patients with laryngeal squamous cell carcinomas. Material and Method: In this study, patients diagnosed with laryngeal squamous cell carcinoma between 2013 and 2015 were recorded from electronic media data and archive preparations were re-evaluated. Patients with consisting of sufficient tumor tissue and reached paraffin blocks were included in the study. Demographic data, histopathological findings, recurrence and survival follow-up were evaluated. p16 immunohistochemical staining was performed on tissue samples containing introductory tumor areas. The p16 staining intensity was evaluated as negative (0), weak (1), moderate (2), or strong (3). The relationship between p16 and all clinicopathological parameters was evaluated. Results: The proportion of patients with stage T4 cancer was found to be p16 negative significantly more frequently than stage T3. Disease-free survival and overall survival were not affected by p16 status (4 groups). However, when p16 status was considered as positive and negative, the p value determined for the effect of p16 status on disease-free survival was found at the limit of significance. Conclusion: There are very different results in the literature on p16 expression. We found an association between complete survival and p16 expression. Upcoming studies with new large series are needed.

Keywords

• Larynx squamous cell carcinoma
• p16 expression
• prognosis

Larinks Skuamöz Hücreli Karsinomlarının Nüks ve Sağkalımında p16 Ekspresyonunun Rolü

Abstract

Amaç: Larinks karsinomlarında en sık görülen histolojik tip skuamöz hücreli karsinomdur. Son yıllarda temel nedensel faktörler arasında insan papilloma virüsü (Human Papilloma Virüs, HPV)’nün de etkili olabileceği bildirilmektedir. HPV enfeksiyonunu tanımlamada immunohistokimyasal olarak p16 proteini değerlendirilmektedir. Bu çalışmanın amacı, laringeal skuamöz hücreli karsinom hastalarında uzun süreli genel sağkalım, hastalıksız sağkalım ve hastalığa özgü hastalıksız sağkalım üzerindeki p16 ekspresyonunun prognostik rolünü değerlendirmektir. Gereç ve Yöntem: Bu çalışmada 2013-2015 yılları arasında larinks skuamöz hücreli karsinom tanısı alan hastalar elektronik ortam verilerinden kayıt edildi ve arşiv preparatları yeniden değerlendirildi. Yeterli tümör dokusu içeren ve parafin bloklarına ulaşılan hastalar çalışmaya dahil edildi. Demografik veriler, histopatolojik bulgular, tümör nüksü, sağkalım takipleri değerlendirildi. Tanıtıcı tümör alanlarını içeren doku örneklerinde p16 immunohistokimyasal boyaması yapıldı. p16 boyama yoğunluğu negatif (0), zayıf (1), orta (2) veya güçlü (3) olarak değerlendirildi. Tüm klinikopatolojik parametreler ile p16 arasındaki ilişki değerlendirildi. Bulgular: Evre T4 kanseri olan hastaların oranı T3 evresine göre anlamlı olarak daha sık p16 negatif olarak saptandı. Hastalıksız sağkalım ve toplam sağkalım p16 durumundan (4 grup) etkilenmedi. Ancak p16 durumu pozitif ve negatif olarak ele alındığında, p16 durumunun hastalıksız sağkalıma etkisi için saptanan p değeri anlamlılık sınırında saptandı. Sonuç: p16 ekspresyonu ile ilgili olarak literatürde çok farklı sonuçlar vardır. Biz tam sağkalım ile p16 ekspresyonu arasında bir ilişki bulduk. Yeni büyük seriler ile yapılacak çalışmalara ihtiyaç vardır.

Keywords

• Larinks skuamöz hücreli karsinomu
• p16 ekspresyonu
• prognoz

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