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TELEPHİUM İMPERATİ'NİN LC-MS/MS İLE POLİFENOLLERİN TARANMASI VE ANTİOKSİDAN ÖZELLİKLERİ

Year 2024, , 305 - 316, 19.02.2024
https://doi.org/10.33715/inonusaglik.1417799

Abstract

Caryophyllaceae familyasına ait olan Telephium imperati (L.) geleneksel Anadolu halk hekimliğinde kullanılan bitkiler arasında yer alır. Bu çalışmada T. imperati’nin fenolik bileşiklerin profili ve antioksidan aktiviteleri ilk defa araştırıldı. Fenolik bileşimlerini belirlemek için sıvı kromatografi-tandem kütle spektrometresi (LC-MS/MS) sistemi uygulandı. Potansiyel antioksidan aktivitelerini değerlendirmek için spektrofotometre kullanıldı. ABTS (2,2'-azino-di-(3-etilbenzotiyazolin)-6-sülfonik asit)), CUPRAC (Kuprik (Cu2+) iyonlarını indirgeme gücü), DPPH(1,1-difenil-2-pikril-hidrazil) ve FRAP (Ferrik iyonları (Fe3+) indirgeme gücü) testleri uygulanarak T. imperati’nin metanol ekstraktının antioksidan kapasitesi belirlendi. Standart olarak kullanılan BHA, BHT ve Troloks göre T. imperati orta düzeyde antioksidan aktivite göstermiştir. LC-MS/MS ile 25 fenolik bileşik analizinde asetohidroksamik asit, kateşin hidrat, siringik asit, kafeik asit, kuersetin, luteolin, kaempferol belirlenmiştir.

References

  • Apak, R., Güclü, K., Özyürek, M. ve Celik, S. E. (2008). Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay. Microchimica Acta, 160(4), 413–419.
  • Apak, R., Güçlü, K., Özyürek, M. ve Karademir, S. E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of agricultural and food chemistry, 52(26), 7970–7981.
  • Benzie, I. F. F. ve Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry, 239(1), 70–76.
  • Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199–1200. doi:10.1038/1811199a0
  • Büyüktuncel, E. (2013). Toplam fenolik içerik ve antioksidan kapasite tayininde kullanılan başlıca spektrofotometrik yöntemler. Marmara Pharmaceutical Journal, 17(2), 93–103.
  • Davis, P. (1967). Flora Of Turkey And The East Aegean Islands, Vol. 2, Edinburgh Univ. Pres, Edinburgh.
  • Dube, A., Nicolazzo, J. A. ve Larson, I. (2010). Chitosan nanoparticles enhance the intestinal absorption of the green tea catechins (+)-catechin and (−)-epigallocatechin gallate. European Journal of Pharmaceutical Sciences, 41(2), 219–225.
  • Flouchi, R., El Far, M., El Atrache, N. E., El Kassmi, S., Ezzarouali, Y., Benkhaira, N., … Fikri-Benbrahim, K. (2023). Ethnobotanical survey on plants used during the COVID-19 pandemic in Taza (Morocco) and population satisfaction according to the" Rules of Association" approach. Journal of Pharmacy & Pharmacognosy Research, 11(3), 455–472.
  • Gülçin, İ. (2006). Antioxidant activity of caffeic acid (3, 4-dihydroxycinnamic acid). Toxicology, 217(2–3), 213–220.
  • Gülçin, İ. ve Beydemir, Ş. (2013). Phenolic compounds as antioxidants: carbonic anhydrase isoenzymes inhibitors. Mini reviews in medicinal chemistry, 13(3), 408–430.
  • Güzel, A. (2023). Relationship Between Phenolic Content Determined by LC‐MS/MS and Antioxidant Capacity and Enzyme Inhibition of Cyclotrichium Niveum L. Chemistry & Biodiversity, e202300027.
  • Güzel, A. (2024). Assessment of antioxidant, acetylcholinesterase, paraoxonase inhibition activities and phenolic content of Alchemilla lithophila. Pakistan Journal of Pharmaceutical Sciences, 37(1), 025-023.
  • Kantar, C., Baltas, N., Karaoglu, S. A. ve Sasmaz, S. (2018). Some azo dyes containing eugenol and guaiacol, synthesis, antioxidant capacity, urease inhibitory properties and anti-helicobacter pylori activity. Revue Roumaine De Chimie, 63(3), 189–197.
  • Meir, S., Kanner, J., Akiri, B. ve Philosoph-Hadas, S. (1995). Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. Journal of agricultural and food chemistry, 43(7), 1813–1819.
  • Michaelidou, A., Hadjipavlou-Litina, D., Matsini, I. ve Tsitsogianni, E. (2007). Heterocyclic aryl (phenyl) acetic acid and aryl acetohydroxamic acids as antiinflammatory-antioxidant agents and inhibitors of lipoxygenase and serine proteases. Medicinal Chemistry, 3(5), 439–445.
  • Munin, A. ve Edwards-Lévy, F. (2011). Encapsulation of natural polyphenolic compounds; a review. Pharmaceutics, 3(4), 793–829.
  • Nejjari, R., Benabbes, M., Amrani, M., Meddah, B., Bouatia, M. ve Taoufik, J. (2019). Phytochemical screening and wound healing activity of Telephium imperati (L.) in rats. South African Journal of Botany, 123, 147–151.
  • Oh, W. Y. ve Shahidi, F. (2017). Lipophilization of Resveratrol and Effects on Antioxidant Activities. Journal of Agricultural and Food Chemistry, 65(39), 8617–8625. doi:10.1021/acs.jafc.7b03129
  • Oyaizu, M. (1986). Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese Journal of Nutrition and Dietetics, 44(6), 307–315. doi:10.5264/eiyogakuzashi.44.307
  • Özgökçe, F. ve Özçelik, H. (2004). Ethnobotanical aspects of some taxa in East Anatolia, Turkey. Economic Botany, 58(4), 697–704.
  • Rajasekaran, A., Sivakumar, V. ve Darlinquine, S. (2012). Evaluation of wound healing activity of Ammannia baccifera and Blepharis maderaspatensis leaf extracts on rats. Revista Brasileira de Farmacognosia, 22, 418–427.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. ve Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9–10), 1231–1237. doi:10.1016/S0891-5849(98)00315-3
  • Shui, G. ve Leong, L. P. (2006). Residue from star fruit as valuable source for functional food ingredients and antioxidant nutraceuticals. Food chemistry, 97(2), 277–284.
  • Tetik, F., Civelek, S. ve Cakilcioglu, U. (2013). Traditional uses of some medicinal plants in Malatya (Turkey). Journal of ethnopharmacology, 146(1), 331–346.
  • Uğur, Y. ve Güzel, A. (2023). Determination of phytochemical content by LC-MS/MS, investigation of antioxidant capacity, and enzyme inhibition effects of nettle (Urtica dioica). European Review for Medical and Pharmacological Sciences, 27(5), 1793–1800.
  • Ugur, Y., Karaaslan-Ayhan, N., Icen, M. S., Bicim, T., Erdogan, S. ve Yaman, M. (2021). Determination of fatty acids in Allium tuncelianum (Tunceli garlic) by gas chromatography with flame ionization detection (GC–FID). Instrumentation Science & Technology, 50(3), 233–239.
  • Yılmaz, U., Şalva, E., Karaat, F. ve Erdoğan, S. (2023). Antiproliferative properties and evaluation of antioxidant of different cornelian cherry genotypes and analysis of phenolic and sugar compounds by HPLC. Hacettepe Journal of Biology and Chemistry, 51(1), 57–70.

Polyphenol Screening by LC-MS/MS and Antioxidant Properties of Telephium imperati

Year 2024, , 305 - 316, 19.02.2024
https://doi.org/10.33715/inonusaglik.1417799

Abstract

Telephium imperati (L.), belonging to the Caryophyllaceae family, is among the plants used in traditional Anatolian folk medicine. In this study, the profile of phenolic compounds and antioxidant activities of T. imperati were investigated for the first time. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) system was applied to determine phenolic compositions. Spectrophotometry was used to evaluate potential antioxidant activities. The antioxidant capacity of the methanol extract of T. imperati was determined by applying ABTS (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid)), CUPRAC (reducing power of Cupric (Cu2+) ions), DPPH(1,1-diphenyl-2-picryl-hydrazyl), and FRAP (Ferric ions (Fe3+) reducing power) tests. Compared to BHA, BHT and Trolox used as standards, T. imperati showed moderate antioxidant activity. In the analysis of 25 phenolic compounds by LC-MS/MS, acetohydroxamic acid, catechin hydrate, syringic acid, caffeic acid, quercetin, luteolin, and kaempferol were determined.

References

  • Apak, R., Güclü, K., Özyürek, M. ve Celik, S. E. (2008). Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay. Microchimica Acta, 160(4), 413–419.
  • Apak, R., Güçlü, K., Özyürek, M. ve Karademir, S. E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of agricultural and food chemistry, 52(26), 7970–7981.
  • Benzie, I. F. F. ve Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry, 239(1), 70–76.
  • Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199–1200. doi:10.1038/1811199a0
  • Büyüktuncel, E. (2013). Toplam fenolik içerik ve antioksidan kapasite tayininde kullanılan başlıca spektrofotometrik yöntemler. Marmara Pharmaceutical Journal, 17(2), 93–103.
  • Davis, P. (1967). Flora Of Turkey And The East Aegean Islands, Vol. 2, Edinburgh Univ. Pres, Edinburgh.
  • Dube, A., Nicolazzo, J. A. ve Larson, I. (2010). Chitosan nanoparticles enhance the intestinal absorption of the green tea catechins (+)-catechin and (−)-epigallocatechin gallate. European Journal of Pharmaceutical Sciences, 41(2), 219–225.
  • Flouchi, R., El Far, M., El Atrache, N. E., El Kassmi, S., Ezzarouali, Y., Benkhaira, N., … Fikri-Benbrahim, K. (2023). Ethnobotanical survey on plants used during the COVID-19 pandemic in Taza (Morocco) and population satisfaction according to the" Rules of Association" approach. Journal of Pharmacy & Pharmacognosy Research, 11(3), 455–472.
  • Gülçin, İ. (2006). Antioxidant activity of caffeic acid (3, 4-dihydroxycinnamic acid). Toxicology, 217(2–3), 213–220.
  • Gülçin, İ. ve Beydemir, Ş. (2013). Phenolic compounds as antioxidants: carbonic anhydrase isoenzymes inhibitors. Mini reviews in medicinal chemistry, 13(3), 408–430.
  • Güzel, A. (2023). Relationship Between Phenolic Content Determined by LC‐MS/MS and Antioxidant Capacity and Enzyme Inhibition of Cyclotrichium Niveum L. Chemistry & Biodiversity, e202300027.
  • Güzel, A. (2024). Assessment of antioxidant, acetylcholinesterase, paraoxonase inhibition activities and phenolic content of Alchemilla lithophila. Pakistan Journal of Pharmaceutical Sciences, 37(1), 025-023.
  • Kantar, C., Baltas, N., Karaoglu, S. A. ve Sasmaz, S. (2018). Some azo dyes containing eugenol and guaiacol, synthesis, antioxidant capacity, urease inhibitory properties and anti-helicobacter pylori activity. Revue Roumaine De Chimie, 63(3), 189–197.
  • Meir, S., Kanner, J., Akiri, B. ve Philosoph-Hadas, S. (1995). Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. Journal of agricultural and food chemistry, 43(7), 1813–1819.
  • Michaelidou, A., Hadjipavlou-Litina, D., Matsini, I. ve Tsitsogianni, E. (2007). Heterocyclic aryl (phenyl) acetic acid and aryl acetohydroxamic acids as antiinflammatory-antioxidant agents and inhibitors of lipoxygenase and serine proteases. Medicinal Chemistry, 3(5), 439–445.
  • Munin, A. ve Edwards-Lévy, F. (2011). Encapsulation of natural polyphenolic compounds; a review. Pharmaceutics, 3(4), 793–829.
  • Nejjari, R., Benabbes, M., Amrani, M., Meddah, B., Bouatia, M. ve Taoufik, J. (2019). Phytochemical screening and wound healing activity of Telephium imperati (L.) in rats. South African Journal of Botany, 123, 147–151.
  • Oh, W. Y. ve Shahidi, F. (2017). Lipophilization of Resveratrol and Effects on Antioxidant Activities. Journal of Agricultural and Food Chemistry, 65(39), 8617–8625. doi:10.1021/acs.jafc.7b03129
  • Oyaizu, M. (1986). Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. The Japanese Journal of Nutrition and Dietetics, 44(6), 307–315. doi:10.5264/eiyogakuzashi.44.307
  • Özgökçe, F. ve Özçelik, H. (2004). Ethnobotanical aspects of some taxa in East Anatolia, Turkey. Economic Botany, 58(4), 697–704.
  • Rajasekaran, A., Sivakumar, V. ve Darlinquine, S. (2012). Evaluation of wound healing activity of Ammannia baccifera and Blepharis maderaspatensis leaf extracts on rats. Revista Brasileira de Farmacognosia, 22, 418–427.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. ve Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9–10), 1231–1237. doi:10.1016/S0891-5849(98)00315-3
  • Shui, G. ve Leong, L. P. (2006). Residue from star fruit as valuable source for functional food ingredients and antioxidant nutraceuticals. Food chemistry, 97(2), 277–284.
  • Tetik, F., Civelek, S. ve Cakilcioglu, U. (2013). Traditional uses of some medicinal plants in Malatya (Turkey). Journal of ethnopharmacology, 146(1), 331–346.
  • Uğur, Y. ve Güzel, A. (2023). Determination of phytochemical content by LC-MS/MS, investigation of antioxidant capacity, and enzyme inhibition effects of nettle (Urtica dioica). European Review for Medical and Pharmacological Sciences, 27(5), 1793–1800.
  • Ugur, Y., Karaaslan-Ayhan, N., Icen, M. S., Bicim, T., Erdogan, S. ve Yaman, M. (2021). Determination of fatty acids in Allium tuncelianum (Tunceli garlic) by gas chromatography with flame ionization detection (GC–FID). Instrumentation Science & Technology, 50(3), 233–239.
  • Yılmaz, U., Şalva, E., Karaat, F. ve Erdoğan, S. (2023). Antiproliferative properties and evaluation of antioxidant of different cornelian cherry genotypes and analysis of phenolic and sugar compounds by HPLC. Hacettepe Journal of Biology and Chemistry, 51(1), 57–70.
There are 27 citations in total.

Details

Primary Language Turkish
Subjects Traditional, Complementary and Integrative Medicine (Other)
Journal Section Araştırma Makalesi
Authors

Abdussamat Güzel 0000-0001-7810-4510

Early Pub Date February 11, 2024
Publication Date February 19, 2024
Submission Date January 10, 2024
Acceptance Date February 8, 2024
Published in Issue Year 2024

Cite

APA Güzel, A. (2024). TELEPHİUM İMPERATİ’NİN LC-MS/MS İLE POLİFENOLLERİN TARANMASI VE ANTİOKSİDAN ÖZELLİKLERİ. İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi, 12(1), 305-316. https://doi.org/10.33715/inonusaglik.1417799