PTZ kindling modeli: Ramelteon'un etkisi altında EEG faktörü ve biyokimya parametrelerinin değerlendirilmesi

Year 2024, , 843 - 859, 21.10.2024

Mukaddes Pala , Ramazan Kozan , Hayrullah Köse , Şenay Görücü Yılmaz

https://doi.org/10.33715/inonusaglik.1495324

Abstract

Pek çok seçici sentetik melatonin reseptörü agonisti, antikonvülsan/anti-epileptojenik özelliklere sahiptir. Bu agonistler melatonin reseptörü 1 (MT1) ve melatonin reseptörü 2'ye (MT2) bağlanarak onların aktivasyonlarına neden olmaktadır. Bu nedenle, bir melatonin agonisti olarak Ramelteon'un (RMLT) antikonvülsan etkisini PTZ (Pentilenetetrazol)-kindling modelinde değerlendirdik. Çalışmada 36 adet erkek Wistar Albino sıçan grupta (Sham, PTZ, dimetilsülfoksit (DMSO), Valproik asit (VPA) (150 mg/kg) + PTZ, RMLT (30 mg/kg)+PTZ, VPA+RMLT+PTZ) değerlendirildi. Tüm gruplar için kortikal elektroensefalografi (EEG) verileri kaydedildi. Nöbetler Racine skalasına göre skorlandı. EEG traselerinde nöbet skorları ve ilk miyoklonik hareketlerin başlangıç zamanları karşılaştırıldı. Serum örneklerinde toplam antioksidan durum (TAS), toplam oksidan durum (TOS), katalaz, miyeloperoksidaz (MPO) ve Tiyol seviyeleri ölçüldü. Ayrıca beyin dokusu örneklerinde Kalsinörin (CaN), Nöropeptid-Y (NPY), Nöron Spesifik Enolaz (NSE) ve S100B seviyeleri ölçüldü. PTZ ve PTZ+Valproik asit+RMLT grupları arasında Racine'in konvülsiyon evrelerinde ilk miyoklonik hareketlerin başlangıcı ve EEG traselerindeki ani artış oranları açısından anlamlı bir fark vardı (P<0.001). Biyokimyasal parametreler, gruplar arasında anlamlı değildi (P > 0.05). RMLT antikonvülsan özelliklere sahiptir. Ek olarak RMLT'nin reseptör tercihi de araştırılabilir.

Keywords

Antikonvülsan etki, Elektroensefalografi, Epilepsi, Pentilentetrazol, Ramelteon

Project Number

12.2011/15

PTZ KINDLING MODEL: EVALUATION OF EEG FACTOR AND BIOCHEMISTRY PARAMETERS UNDER THE INFLUENCE OF RAMELTEON

Abstract

Many selective synthetic melatonin receptor agonists have anticonvulsant/anti-epileptogenic properties. These agonists bind to melatonin receptor 1 (MT1) and receptor 2 (MT2), causing their activation. Therefore, we evaluated the anticonvulsant effect of Ramelteon (RMLT) as a melatonin agonist in the PTZ (Pentylenetetrazol)-kindling model. In the study, 36 male Wistar Albino rats were assessed in 6 groups (Sham, PTZ, dimethylsulphoxide (DMSO), Valproic acid (VPA) (150 mg/kg) + PTZ, RMLT (30 mg/kg)+PTZ, VPA+RMLT+PTZ). Cortical electroencephalography (EEG) data were recorded for all groups. Seizures were scored according to the Racine scale. Seizure scores and onset times of the first myoclonic movements were compared in EEG traces. Total antioxidant status (TAS), total oxidant status (TOS), catalase, myeloperoxidase (MPO), and Thiol levels were measured in serum samples. Also, Calcineurin (CaN), Neuropeptide-Y (NPY), Neuron Specific Enolase (NSE), and S100B levels were measured in brain tissue samples. There was a significant difference between the PTZ and PTZ+Valproic acid+RMLT groups for the onset of the first myoclonic movements and the rate of spikes in the EEG traces in Racine's convulsion stages (P<0.001). Biochemical parameters were not significant between the groups (P > 0.05). RMLT has anticonvulsant properties. Additionally, the receptor preference of RMLT can be investigated.

Keywords

Anticonvulsant effect, Electroencephalography, Epilepsy, Pentylenetetrazole, Ramelteon

Ethical Statement

Study permission was obtained from Bezmialem Vakif University Experimental Animals Local Ethics Committee (2011/51).

Supporting Institution

Bezmialem Vakıf Üniversitesi

Project Number

12.2011/15

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