ANALYSIS OF IL-1Ra AND IL-4 GENE VNTRS POLYMORPHISMS AMONG DENTAL LABORATORY TECHNICIANS: A GENOTYPE-PHENOTYPE STUDY

Year 2021, , 831 - 845, 29.11.2021

Burcu Yüksel , Serap Yıldırım

https://doi.org/10.33715/inonusaglik.849794

Cited By: 1

Abstract

For people working in dental prosthesis laboratories (DPL), metal alloys and methylmethacrylate-based monomers and polymers used in the laboratory can pose occupational risks. These risks can cause occupational health problems and toxic effects on human health. This study purposes of researching the effects of occupational exposure because of dental prosthesis manufacturing operations on variable number tandem repeat (VNTR) polymorphisms in IL-1Ra and IL-4 genes. 29 DPL workers, the experimental group, were given a questionnaire, including the symptoms that might arise due to occupational exposure, and their responses were compared with 30 participants as a control group. Genotype distributions and allele frequencies for IL-1Ra intron 2 (rs2234663) and IL-4 intron 3 (rs79071878) VNTR polymorphisms were analyzed using the PCR technique. Both IL-1Ra intron 2 and IL-4 intron 3 polymorphisms identified from the experimental and control groups were not statistically significantly different (p >.05). However, runny nose, burning throat, dyspnea and dryness and rash on the skin were significantly different when DPL workers were compared with the control group according to logistic regression analysis (p <.05). No statistical difference was found in other phenotypic characters. However, due to the limited sample size, our results need to be confirmed in large-scale studies.

Keywords

Dental Laboratory Technicians, interleukin-1 receptor antagonist, interleukin-4, Occupational health and safety, polymorphism

Diş Laboratuvarı Teknisyenlerinde IL-1Ra ve IL-4 Geni VNTR Polimorfizmlerinin Analizi: Genotip-Fenotip Çalışması

Abstract

Diş protez laboratuvarlarında (DPL) çalışan kişiler için laboratuvarda kullanılan metal alaşımları ve metilmetakrilat bazlı monomerler ve polimerler mesleki risk oluşturabilmektedir. Bu riskler mesleğe bağlı sağlık problemlerine ve insan sağlığında toksik etkilere sebep olabilir. Bu çalışmanın amacı dental protez üretim operasyonlarına bağlı mesleki maruziyetin IL-1Ra ve IL-4 genlerindeki değişken numaralı tandem tekrar (VNTR) polimorfizmleri üzerindeki etkilerini araştırmaktır. 29 DPL çalışanı deney grubu; 30 kontrol grubu ile karşılaştırılarak mesleki maruziyetlerine bağlı ortaya çıkacak semptomları da içine alan anket formları uygulanmıştır. IL-1Ra intron 2 (rs2234663) ve IL-4 intron 3 (rs79071878) VNTR polimorfizmleri için genotip dağılımları ve allel frekansları PCR tekniği kullanılarak analiz edilmiştir. Deney grubu ve kontrol grubundan belirlenen hem IL-1Ra intron 2 hem de IL-4 intron 3 polimorfizmleri istatistiksel olarak anlamlı bulunmamıştır (p >.05). Ancak DPL çalışanları lojistik regresyon analizine göre kontrol grubu ile karşılaştırıldığında burun akıntısı, boğaz yanması, nefes darlığı ve ciltte kuruluk ve kızarıklık gibi alanlarda anlamlı farklılıklar bulundu (p <.05). Bununla birlikte, sınırlı örneklem büyüklüğü nedeniyle, sonuçlarımızın büyük ölçekli çalışmalarda doğrulanması gerekmektedir.

Keywords

Diş Laboratuvarı Teknisyenleri, interlökin-1 reseptör antagonisti, interlökin-4, iş sağlığı, polimorfizm

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