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Beyin Hasarı ile Restoratif Materyaller Arasındaki Bağlantının İncelenmesi: Bir İn-vivo Çalışma

Year 2020, Volume: 8 Issue: 3, 664 - 675, 30.11.2020
https://doi.org/10.33715/inonusaglik.718551

Abstract

Bu çalışmanın amacı, rezin kompozit, amalgam ve cam iyonomer gibi restoratif materyallerin beyin dokusu üzerindeki olası etkilerini araştırmaktır. Otuziki adet Wistar albino sıçan alındı ve restoratif malzemeler boyun bölgesinin arkasına yerleştirildi. 8 hafta sonra beyin dokuları çıkarıldı ve nükleer faktör kappa B (NF-Κb)/p65 ve hematoksilin & eosin boyama metodları ile analiz yapıldı. Ayrıca, beyin dokusunda; süperoksit dismutaz, katalaz, lipid peroksidaz ve glutatyon düzeyleri tespit edildi. Deney gruplarında kontrol gruplarına göre immünpozitif hücre boyanması daha yoğun olarak gözlendi, ayrıca bu gruplarda nöronal dejenerasyon saptandı. Test sonuçları, tüm restoratif materyal gruplarında kontrol grubuna kıyasla artmış oksidatif stres gösterdi. Kullanılan restoratif materyallerin üçü de beyin dokusu üzerinde ciddi sitotoksik etkilere neden oldu. Ek olarak, beyin dokusunda oksidatif stres oluşmuş olabilir. Restoratif maddelerinin içerdikleri ağır metal bileşikler, restoratif maddelerin sitotoksik etkilerini gösterecek şekilde nöronal dejenerasyona neden oldu ve ayrıca oksidatif strese yol açmış olabilirler.

Supporting Institution

TÜBİTAK

Project Number

114S881

References

  • Al-Khafaji, T. G. H., Al-Timimi, A. H. O., Abbas, A. S., Alanz, M. A. A., Murshedi, S. J. K., & Alam, M. K. (2020). Mercury loss from dental amalgam fillings. Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 20, 5267.
  • Aranha, A. M., Giro, E. M., Hebling, J., Lessa, F. C., & Costa, C. A. (2010). Effects of light-curing time on the cytotoxicity of a restorative composite resin on odontoblast-like cells. J Appl Oral Sci, 18(5), 461-466.
  • Campbell, A., Kumar, A., La Rosa, F. G., Prasad, K. N., & Bondy, S. C. (2000). Aluminum Increases levels of β‐amyloid and ubiquitin in neuroblastoma but not in glioma cells. Proceedings of the Society for Experimental Biology and Medicine, 223(4), 397-402.
  • Campbell, A., Prasad, K. N., & Bondy, S. C. (1999). Aluminum-induced oxidative events in cell lines: glioma are more responsive than neuroblastoma. Free Radical Biology and Medicine, 26(9), 1166-1171.
  • Christensen, G. J. (1990). Glass ionomer as a luting material, The Journal of the American Dental Association, 120(1), 59-62.
  • Crapper, D. R., Quittkat, S., Krishnan, S. S., Dalton, A. J., & De Boni, U. (1980). Intranuclear aluminum content in Alzheimer's disease, dialysis encephalopathy, and experimental aluminum encephalopathy, Acta Neuropathol, 50(1), 19-24.
  • Donly, K. J., & Henson, T. (2005). Glass ionomer cement restorations for contemporary pediatric dentistry. Alpha Omegan, 98(4), 21-25.
  • Garcia, J. J., Reiter, R. J., Guerrero, J. M., Escames, G., Yu, B. P., Oh, C. S., & Munoz-Hoyos, A. (1997). Melatonin prevents changes in microsomal membrane fluidity during induced lipid peroxidation. FEBS Lett, 408(3), 297-300.
  • Glick, M., Monteiro da Silva, O., Seeberger, G. K., Xu, T., Pucca, G., Williams, D. M., . . . Séverin, T. (2012). FDI Vision 2020: shaping the future of oral health. International dental journal, 62(6), 278-291.
  • Guest, J. A., & Grant, R. S. (2012). Effects of dietary derived antioxidants on the central nervous system. International Journal of Nutrition, Pharmacology, Neurological Diseases, 2(3), 185.
  • Kamendulis, L. M., Jiang, J., Xu, Y., & Klaunig, J. E. (1999). Induction of oxidative stress and oxidative damage in rat glial cells by acrylonitrile. Carcinogenesis, 20(8), 1555-1560.
  • Lammeier, C., Li, Y., Lunos, S., Fok, A., Rudney, J., & Jones, R. S. (2012). Influence of dental resin material composition on cross-polarization-optical coherence tomography imaging. J Biomed Opt, 17(10), 106002. doi:10.1117/1.JBO.17.10.106002
  • Miyatake, M., Miyagawa, K., Mizuo, K., Narita, M., & Suzuki, T. (2006). Dynamic changes in dopaminergic neurotransmission induced by a low concentration of bisphenol‐a in neurones and astrocytes. Journal of neuroendocrinology, 18(6), 434-444.
  • Nakajima, H., Komatsu, H., & Okabe, T. (1997). Aluminum ions in analysis of released fluoride from glass ionomers. J Dent, 25(2), 137-144.
  • Needleman, H. L. (2006). Mercury in dental amalgam—a neurotoxic risk? Jama, 295(15), 1835-1836.
  • Omar, B. A., Flores, S. C., & McCord, J. M. (1992). Superoxide dismutase: pharmacological developments and applications. Adv Pharmacol, 23, 109-161.
  • Park, J. D., & Zheng, W. (2012). Human exposure and health effects of inorganic and elemental mercury. J Prev Med Public Health, 45(6), 344-352. doi:10.3961/jpmph.2012.45.6.344
  • Petersen, P. E., Baez, R., Kwan, S., & Ogawa, H. (2009). Future use of materials for dental restoration. World Health Organization: Geneva, Switzerland.
  • Pizzichini, M., Fonzi, M., Sugherini, L., Fonzi, L., Gasparoni, A., Comporti, M., & Pompella, A. (2002). Release of mercury from dental amalgam and its influence on salivary antioxidant activity. Sci Total Environ, 284(1-3), 19-25.
  • Polat, B., Albayrak, Y., Suleyman, B., Dursun, H., Odabasoglu, F., Yigiter, M., . . . Suleyman, H. (2011). Antiulcerative effect of dexmedetomidine on indomethacin-induced gastric ulcer in rats. Pharmacol Rep, 63(2), 518-526.
  • Ratcliffe, H. E., Swanson, G. M., & Fischer, L. J. (1996). Human exposure to mercury: a critical assessment of the evidence of adverse health effects. J Toxicol Environ Health, 49(3), 221-270. doi:10.1080/713851079
  • Rossi, A. D., Viviani, B., Zhivotovsky, B., Manzo, L., Orrenius, S., Vahter, M., & Nicotera, P. (1997). Inorganic mercury modifies Ca2+ signals, triggers apoptosis and potentiates NMDA toxicity in cerebellar granule neurons. Cell Death Differ, 4(4), 317-324. doi:10.1038/sj.cdd.4400244
  • Thompson, C. M., Markesbery, W. R., Ehmann, W. D., Mao, Y. X., & Vance, D. E. (1988). Regional brain trace-element studies in Alzheimer's disease. Neurotoxicology, 9(1), 1-7.
  • Tillberg, A., Jarvholm, B., & Berglund, A. (2008). Risks with dental materials. Dent Mater, 24(7), 940-943. doi:10.1016/j.dental.2007.11.009
  • Tong, J., Fitzmaurice, P. S., Moszczynska, A., Rathitharan, G., Ang, L. C., Meyer, J. H.,… Kish, S. J. (2018). Normal glutathione levels in autopsied brain of chronic users of heroin and of cocaine. Drug and alcohol dependence, 190, 20–28. https://doi.org/10.1016/j.drugalcdep.2018.05.021
  • Uikey, A., Hazarey, V., & Vaidhya, S. (2003). Estimation of serum antioxidant enzymes superoxide dismutase and glutathione peroxidase in oral submucous fibrosis: A biochemical study. Journal of Oral and Maxillofacial Pathology, 7, 44.
  • Uzer Celik E., C. B., Tunac AT. (2016). Dental caries and caries associated factors of six and seven year-old children living in a high fluoride area. Cumhuriyet Dent J, 19(2), 135-144.
  • Uzkeser, H., Cadirci, E., Halici, Z., Odabasoglu, F., Polat, B., Yuksel, T. N., . . . Atalay, F. (2012). Anti-inflammatory and antinociceptive effects of salbutamol on acute and chronic models of inflammation in rats: involvement of an antioxidant mechanism. Mediators Inflamm, 2012, 438912. doi:10.1155/2012/438912

THE EXAMINATION OF THE RELATIONSHIP BETWEEN BRAIN DAMAGE AND DENTAL RESTORATIVE FILLING MATERIALS: AN IN-VIVO STUDY

Year 2020, Volume: 8 Issue: 3, 664 - 675, 30.11.2020
https://doi.org/10.33715/inonusaglik.718551

Abstract

The aim of this study is to investigate the possible effects of restorative materials such as resin composite, amalgam and glass ionomer on brain tissue. Thirty-two Wistar albino rats were taken and restorative materials were put in the neck region at the back. After 8 weeks, the brain tissues were removed and analyzed using nuclear factor kappa B (NF-κB)/p65 and hematoxylin & eosin staining methods. Further, the levels of superoxide dismutase, catalase, lipid peroxidase, and glutathione were determined in the brain tissues. More intense staining of immunopositive cells was observed in the restorative material groups than the control group, also neuronal degeneration was detected in these groups. Results of the tests indicated increased oxidative stress in all the restorative material groups compared with the control group. All three dental restorative materials exhibited cytotoxic effects on the brain tissue. Additionally, oxidative stress may have occurred in the brain tissue. The heavy metal compounds in the restorative materials caused neuronal degeneration and may also have caused oxidative stress in the brain tissue, indicating the cytotoxic effects of dental restorative materials.

Project Number

114S881

References

  • Al-Khafaji, T. G. H., Al-Timimi, A. H. O., Abbas, A. S., Alanz, M. A. A., Murshedi, S. J. K., & Alam, M. K. (2020). Mercury loss from dental amalgam fillings. Pesquisa Brasileira em Odontopediatria e Clínica Integrada, 20, 5267.
  • Aranha, A. M., Giro, E. M., Hebling, J., Lessa, F. C., & Costa, C. A. (2010). Effects of light-curing time on the cytotoxicity of a restorative composite resin on odontoblast-like cells. J Appl Oral Sci, 18(5), 461-466.
  • Campbell, A., Kumar, A., La Rosa, F. G., Prasad, K. N., & Bondy, S. C. (2000). Aluminum Increases levels of β‐amyloid and ubiquitin in neuroblastoma but not in glioma cells. Proceedings of the Society for Experimental Biology and Medicine, 223(4), 397-402.
  • Campbell, A., Prasad, K. N., & Bondy, S. C. (1999). Aluminum-induced oxidative events in cell lines: glioma are more responsive than neuroblastoma. Free Radical Biology and Medicine, 26(9), 1166-1171.
  • Christensen, G. J. (1990). Glass ionomer as a luting material, The Journal of the American Dental Association, 120(1), 59-62.
  • Crapper, D. R., Quittkat, S., Krishnan, S. S., Dalton, A. J., & De Boni, U. (1980). Intranuclear aluminum content in Alzheimer's disease, dialysis encephalopathy, and experimental aluminum encephalopathy, Acta Neuropathol, 50(1), 19-24.
  • Donly, K. J., & Henson, T. (2005). Glass ionomer cement restorations for contemporary pediatric dentistry. Alpha Omegan, 98(4), 21-25.
  • Garcia, J. J., Reiter, R. J., Guerrero, J. M., Escames, G., Yu, B. P., Oh, C. S., & Munoz-Hoyos, A. (1997). Melatonin prevents changes in microsomal membrane fluidity during induced lipid peroxidation. FEBS Lett, 408(3), 297-300.
  • Glick, M., Monteiro da Silva, O., Seeberger, G. K., Xu, T., Pucca, G., Williams, D. M., . . . Séverin, T. (2012). FDI Vision 2020: shaping the future of oral health. International dental journal, 62(6), 278-291.
  • Guest, J. A., & Grant, R. S. (2012). Effects of dietary derived antioxidants on the central nervous system. International Journal of Nutrition, Pharmacology, Neurological Diseases, 2(3), 185.
  • Kamendulis, L. M., Jiang, J., Xu, Y., & Klaunig, J. E. (1999). Induction of oxidative stress and oxidative damage in rat glial cells by acrylonitrile. Carcinogenesis, 20(8), 1555-1560.
  • Lammeier, C., Li, Y., Lunos, S., Fok, A., Rudney, J., & Jones, R. S. (2012). Influence of dental resin material composition on cross-polarization-optical coherence tomography imaging. J Biomed Opt, 17(10), 106002. doi:10.1117/1.JBO.17.10.106002
  • Miyatake, M., Miyagawa, K., Mizuo, K., Narita, M., & Suzuki, T. (2006). Dynamic changes in dopaminergic neurotransmission induced by a low concentration of bisphenol‐a in neurones and astrocytes. Journal of neuroendocrinology, 18(6), 434-444.
  • Nakajima, H., Komatsu, H., & Okabe, T. (1997). Aluminum ions in analysis of released fluoride from glass ionomers. J Dent, 25(2), 137-144.
  • Needleman, H. L. (2006). Mercury in dental amalgam—a neurotoxic risk? Jama, 295(15), 1835-1836.
  • Omar, B. A., Flores, S. C., & McCord, J. M. (1992). Superoxide dismutase: pharmacological developments and applications. Adv Pharmacol, 23, 109-161.
  • Park, J. D., & Zheng, W. (2012). Human exposure and health effects of inorganic and elemental mercury. J Prev Med Public Health, 45(6), 344-352. doi:10.3961/jpmph.2012.45.6.344
  • Petersen, P. E., Baez, R., Kwan, S., & Ogawa, H. (2009). Future use of materials for dental restoration. World Health Organization: Geneva, Switzerland.
  • Pizzichini, M., Fonzi, M., Sugherini, L., Fonzi, L., Gasparoni, A., Comporti, M., & Pompella, A. (2002). Release of mercury from dental amalgam and its influence on salivary antioxidant activity. Sci Total Environ, 284(1-3), 19-25.
  • Polat, B., Albayrak, Y., Suleyman, B., Dursun, H., Odabasoglu, F., Yigiter, M., . . . Suleyman, H. (2011). Antiulcerative effect of dexmedetomidine on indomethacin-induced gastric ulcer in rats. Pharmacol Rep, 63(2), 518-526.
  • Ratcliffe, H. E., Swanson, G. M., & Fischer, L. J. (1996). Human exposure to mercury: a critical assessment of the evidence of adverse health effects. J Toxicol Environ Health, 49(3), 221-270. doi:10.1080/713851079
  • Rossi, A. D., Viviani, B., Zhivotovsky, B., Manzo, L., Orrenius, S., Vahter, M., & Nicotera, P. (1997). Inorganic mercury modifies Ca2+ signals, triggers apoptosis and potentiates NMDA toxicity in cerebellar granule neurons. Cell Death Differ, 4(4), 317-324. doi:10.1038/sj.cdd.4400244
  • Thompson, C. M., Markesbery, W. R., Ehmann, W. D., Mao, Y. X., & Vance, D. E. (1988). Regional brain trace-element studies in Alzheimer's disease. Neurotoxicology, 9(1), 1-7.
  • Tillberg, A., Jarvholm, B., & Berglund, A. (2008). Risks with dental materials. Dent Mater, 24(7), 940-943. doi:10.1016/j.dental.2007.11.009
  • Tong, J., Fitzmaurice, P. S., Moszczynska, A., Rathitharan, G., Ang, L. C., Meyer, J. H.,… Kish, S. J. (2018). Normal glutathione levels in autopsied brain of chronic users of heroin and of cocaine. Drug and alcohol dependence, 190, 20–28. https://doi.org/10.1016/j.drugalcdep.2018.05.021
  • Uikey, A., Hazarey, V., & Vaidhya, S. (2003). Estimation of serum antioxidant enzymes superoxide dismutase and glutathione peroxidase in oral submucous fibrosis: A biochemical study. Journal of Oral and Maxillofacial Pathology, 7, 44.
  • Uzer Celik E., C. B., Tunac AT. (2016). Dental caries and caries associated factors of six and seven year-old children living in a high fluoride area. Cumhuriyet Dent J, 19(2), 135-144.
  • Uzkeser, H., Cadirci, E., Halici, Z., Odabasoglu, F., Polat, B., Yuksel, T. N., . . . Atalay, F. (2012). Anti-inflammatory and antinociceptive effects of salbutamol on acute and chronic models of inflammation in rats: involvement of an antioxidant mechanism. Mediators Inflamm, 2012, 438912. doi:10.1155/2012/438912
There are 28 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Araştırma Makalesi
Authors

Selina Aksak Karameşe 0000-0002-4820-2096

İsmail Can 0000-0003-2236-9709

Mustafa Duzyol 0000-0002-8438-1423

Nilgün Seven 0000-0002-0241-8334

Mevlüt Albayrak 0000-0001-8673-6577

Fadime Atalay 0000-0002-4504-4352

Esra Düzyol 0000-0002-5674-6990

Cemal Gündoğdu 0000-0003-2857-923X

Project Number 114S881
Publication Date November 30, 2020
Submission Date April 12, 2020
Acceptance Date September 7, 2020
Published in Issue Year 2020 Volume: 8 Issue: 3

Cite

APA Aksak Karameşe, S., Can, İ., Duzyol, M., Seven, N., et al. (2020). THE EXAMINATION OF THE RELATIONSHIP BETWEEN BRAIN DAMAGE AND DENTAL RESTORATIVE FILLING MATERIALS: AN IN-VIVO STUDY. İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi, 8(3), 664-675. https://doi.org/10.33715/inonusaglik.718551