The Effects of Neurotensin Agonist PD149163 on Scopolamine-Induced Learning and Memory Impairment in Mice

Year 2021, Volume: 9 Issue: 2, 720 - 731, 25.06.2021

Ruhan Deniz Topuz , Oktay Kaya

https://doi.org/10.33715/inonusaglik.829609

Abstract

Neurotensin (NT) is a tridecapeptide that acts as a neuromodulator, neurotransmitter and neurohormone in the central nervous system. NT plays a role in physiological processes such as regulation of body temperature, pain, motor activity, learning and memory formation. In this study, it has been planned to investigate the effects of acute and chronic usage of NT agonist PD149163 on scopolamine induced learning and memory impairment This study has been approved by Trakya University Animal Experiments Local Ethics Committee with the decision number 2019.02.01. In the study, a total of 32 male Balb/c mice were divided into 4 groups. Learning and memory functions were evaluated in the Morris water maze test. After 7 days of scopolamine (1 mg / kg i.p.) administration, it was examined whether acute and chronic (4mg/kg, i.p, 7 day) PD149163 improved learning and memory impairment. In the study, the data from swimming trainings were analyzed with repeated measures two-way variance analysis (ANOVA). Probe data were evaluated by using one-way variance analysis. Chronic PD149163 had no effect in either the learning phase or the retention phase in the Morris water maze test. Acute PD149163 had no effect on scopolamine-induced memory impairment. In our study, no curative effect of NT agonist in scopolamine-induced memory impairment has been observed. Although NT receptors are considered as new drug targets, we think that a large number of studies are needed in this field.

Keywords

Hippocampus, Memory, Learning, Neurotensin

FARELERDE SKOPOLAMİNLE BOZULMUŞ ÖĞRENME VE BELLEK ÜZERİNE NÖROTENSİN AGONİSTİ PD149163’ÜN ETKİSİ

Abstract

Nörotensin (NT), santral sinir sisteminde nöromodülatör, nörotransmitter ve nörohormon olarak görev yapan bir tridekapeptittir. NT; vücut sıcaklığının düzenlenmesi, ağrı, motor aktivite, öğrenme ve bellek yapılanması gibi fizyolojik süreçlerde rol oynar. Bu çalışmada NT agonisti PD149163’ün akut ve kronik kullanımının skopolaminle oluşturulmuş bellek bozukluğu üzerine etkilerinin incelenmesi amaçlandı. Bu çalışma Trakya Üniversitesi Hayvan Deneyleri Yerel Etik kurulundan 2019.02.01 karar no ile onaylanmıştır. Çalışmada toplam 32 adet Balb/c türü erişkin erkek fare 4 gruba ayrıldı. Öğrenme ve bellek fonksiyonları Morris su labirenti testinde değerlendirildi. 7 gün boyunca skopolamin (1 mg/kg i.p.) uygulandıktan sonra akut ve kronik (7 gün, 4 mg/kg, i.p) PD149163 tedavisinin öğrenme ve bellek bozukluğunu geri döndürüp döndürmediği incelendi. Çalışmada yüzme eğitimlerinden elde edilen veriler, tekrarlayan ölçümler iki yönlü varyans analizi (ANOVA ) ile analiz edildi. Probe verileri tek yönlü varyans analizi ile değerlendirildi. Kronik PD149163 uygulamasının Morris su labirenti testinde ne öğrenme fazında ne de bellek fazında iyileştirici bir etkisi olmadı. Akut PD149163 uygulamasının skopolaminle oluşturulmuş bellek bozukluğu üzerine herhangi bir etkisi olmadı. Çalışmamızda NT agonistinin skopolaminle oluşturulan öğrenme ve bellek bozukluğunda düzeltici etkisi görülmemiştir. NT reseptörleri yeni ilaç hedefleri olarak değerlendirilmelerine karşın bu alanda yapılacak çok fazla sayıda çalışmaya ihtiyaç olduğu düşüncesindeyiz.

Keywords

Bellek, Hipokampüs, Öğrenme, Nörotensin

Thanks

Araştırmamızda davranış deneylerinin gerçekleşmesinde büyük emeği olan Öğr.Gör. Kübra Duvan AYDEMİR ve Araş. Gör Dilşat ERÜMİT; deneyimlerinden faydalandığımız Prof. Dr. Ahmet ULUGÖL ve Prof. Dr. Ç. Hakan KARADAĞ’a teşekkür ederiz.

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