Eryngium Billardieri F. Dolaroche'den (Boğa Dikeni) Hazırlanan Ekstraktlarin Fenolik İçerikleri Ve Biyolojik Özellikleri

Year 2021, Volume: 9 Issue: 3, 785 - 795, 29.11.2021

Ahmet Savcı

https://doi.org/10.33715/inonusaglik.904506

Cited By: 2

Abstract

Bu çalışmada, Eryngium billardieri (Boğa Dikeni) Ağrı iline bağlı Patnos ilçesinden vejetasyon döneminde toplandı. Toplanan bitkinin bir kısmı -86oC dondurucuda taze olarak depolanırken, diğer bir kısmı ise herbaryum materyali halinde saklandı. Tür tayini yapıldıktan sonra bitkinin taze ve herbaryum numunelerinin toprak üstü kısımlarından metanol ve saf su ekstraktları hazırlandı. Ekstraktların fenolik içeriği HPLC kullanılarak belirlendi. Ayrıca ilgili özütlerin çeşitli in vitro antioksidan özellikleri ve DNA koruyucu özellikleri test edildi. Nihayetinde, elde edilen sonuçlar biribiri ile ilişkilendirilmiştir. Araştırma sonuçları taze bitki ekstraktlarının herbaryum ekstraktlarına göre daha zengin fenolik madde içerdiğini gösterdi. Her iki ekstre iyi antioksidan aktivite gösterse de aktiviteleri standart antioksidanlardan daha düşüktü. Ayrıca herbaryum ekstraktlarının ortamdaki H2O2 varlığından bağımsız olarak, pUC18 DNA stabilizasyonu üzerinde nispeten daha olumlu bir etkiye sahip olduğu görüldü. Literatürde E.billardieri üzerinde yapılan çalışma sayısının sınırlı olduğu göz önüne alınırsa, bu çalışma bu konuda yapılacak çalışmalarda yol gösterici olacaktır.

Keywords

Antioksidan, Apiaceae, DNA, Eryngium billardieri, HPLC

Supporting Institution

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Project Number

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Thanks

Doç. Dr. Murat Kurşat'a teşekkür ederim.

BIOLOGICAL PROPERTIES AND PHENOLIC CONTENTS OF EXTRACTS PREPARED FROM ERYNGIUM BILLARDIERI F. DOLAROCHE

Abstract

In this study, Eryngium billardieri was collected from Patnos district of Ağrı province during the vegetation period. While some of the collected plant was stored fresh in a -86oC freezer, the other part was preserved as herbarium material. After the species of the plant were identified, methanol and pure water extracts were prepared from the aerial parts of the fresh and herbarium samples. The phenolic contents of the extracts were determined by using HPLC. Moreover, various in vitro antioxidant properties and DNA protective properties of the relevant extracts were tested. The comparison of the test results showed that the phenolic compounds of the fresh plant extracts were more than that of herbarium extracts. Although both extracts showed good antioxidant activities, their activity was lower than the standart antioxidants. Moreover, herbarium extracts were seen to have a relatively better effect on the pUC18 DNA stabilization, irrespective of the presence of H2O2 in the environment. Having considered that there is limited number of studies on E.billardieri in the literature, this study will offer important insights into the studies in this area.

Keywords

Antioxidant, Apiaceae, DNA, Eryngium billardieri, HPLC

Project Number

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