TELEPHİUM İMPERATİ'NİN LC-MS/MS İLE POLİFENOLLERİN TARANMASI VE ANTİOKSİDAN ÖZELLİKLERİ

Year 2024, Volume: 12 Issue: 1, 305 - 316, 19.02.2024

Abdussamat Güzel

https://doi.org/10.33715/inonusaglik.1417799

Abstract

Caryophyllaceae familyasına ait olan Telephium imperati (L.) geleneksel Anadolu halk hekimliğinde kullanılan bitkiler arasında yer alır. Bu çalışmada T. imperati’nin fenolik bileşiklerin profili ve antioksidan aktiviteleri ilk defa araştırıldı. Fenolik bileşimlerini belirlemek için sıvı kromatografi-tandem kütle spektrometresi (LC-MS/MS) sistemi uygulandı. Potansiyel antioksidan aktivitelerini değerlendirmek için spektrofotometre kullanıldı. ABTS (2,2'-azino-di-(3-etilbenzotiyazolin)-6-sülfonik asit)), CUPRAC (Kuprik (Cu2+) iyonlarını indirgeme gücü), DPPH(1,1-difenil-2-pikril-hidrazil) ve FRAP (Ferrik iyonları (Fe3+) indirgeme gücü) testleri uygulanarak T. imperati’nin metanol ekstraktının antioksidan kapasitesi belirlendi. Standart olarak kullanılan BHA, BHT ve Troloks göre T. imperati orta düzeyde antioksidan aktivite göstermiştir. LC-MS/MS ile 25 fenolik bileşik analizinde asetohidroksamik asit, kateşin hidrat, siringik asit, kafeik asit, kuersetin, luteolin, kaempferol belirlenmiştir.

Keywords

Antioksidan, LC-MS/MS, Polifenol, Telephium imperati

Polyphenol Screening by LC-MS/MS and Antioxidant Properties of Telephium imperati

Abstract

Telephium imperati (L.), belonging to the Caryophyllaceae family, is among the plants used in traditional Anatolian folk medicine. In this study, the profile of phenolic compounds and antioxidant activities of T. imperati were investigated for the first time. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) system was applied to determine phenolic compositions. Spectrophotometry was used to evaluate potential antioxidant activities. The antioxidant capacity of the methanol extract of T. imperati was determined by applying ABTS (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid)), CUPRAC (reducing power of Cupric (Cu2+) ions), DPPH(1,1-diphenyl-2-picryl-hydrazyl), and FRAP (Ferric ions (Fe3+) reducing power) tests. Compared to BHA, BHT and Trolox used as standards, T. imperati showed moderate antioxidant activity. In the analysis of 25 phenolic compounds by LC-MS/MS, acetohydroxamic acid, catechin hydrate, syringic acid, caffeic acid, quercetin, luteolin, and kaempferol were determined.

Keywords

Antioxidant, LC-MS/MS, Polyphenol, Telephium imperati

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