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Scanning Electron Microscopic Analyses of the Effects of Different Disinfectant Methods on Dentinal Structure

Year 2013, Volume: 6 Issue: 2, 65 - 68, 01.06.2013

Abstract

The purpose of this study was to evaluate the prepared dentin surface and to determine whether or not it is possible to seal the dentinal tubules, after different cavity disinfectant applications. Thirty mandibular molars were sectioned parallel to the occlusal plane to expose the midcoronal dentin. All of the teeth were divided into five groups (n = 6 per group): (1) in group OZ, the dentin surfaces were exposed to ozone gas from the Ozonytron XP delivery system, (2) in group ED, the dentin surfaces were irradiated with an Er:YAG laser, (3) in group ND, the dentin surfaces were irradiated with an Nd:YAG laser, (4) in group CHX, the dentin surfaces were treated with a 2% chlorhexidine solution, and (5) in the control group, no treatment was applied. Significant differences among the test groups were also observed in the scanning electron microscopy evaluation (P < .05). Scanning electron microscopy showed that the effect of laser energy on dentin varied from cratering, poring, fissuring, fracturing and cracking up to melting; also, the dentinal tubules were not sealed, in contrast with the control group. No formation of smear layer and open dentinal tubules were obtained by using Er:YAG laser irradiation. Clinical article(J Int Dent Med Res 2013; 6: (2), pp. 65-68)

References

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  • Ersin NK, Candan U, Aykut A, Eronat C, Belli S. No adverse effect to bonding following caries disinfection with chlorhexidine. J Dent Child (Chic) 2009;76(1):20-7.
  • Brannstr.M, Johnson G. Effects of Various Conditioners and Cleaning Agents on Prepared Dentin Surfaces - Scanning Electron-Microscopic Investigation. Journal of Prosthetic Dentistry 1974;31(4):422-30.
  • Schultz RJ, Harvey GP, Fernandezberos ME, et al. Bactericidal Effects of the Neodymium Yag Laser - Invitro Study. Lasers in Surgery and Medicine 1986;6(5):445-48.
  • Yamayoshi T, Tatsumi N. Microbicidal effects of ozone solution on methicillin-resistant Staphylococcus aureus. Drugs Exp Clin Res 1993;19(2):59-64.
  • Bocci V, Luzzi E, Corradeschi F, Paulesu L, Distefano A. Studies on the Biological Effects of Ozone .3. An Attempt to Define Conditions for Optimal Induction of Cytokines. Lymphokine and Cytokine Research 1993;12(2):121-26.
  • Gurgan S, Bolay S, Kiremitci A. Effect of disinfectant application methods on the bond strength of composite to dentin. Journal of Oral Rehabilitation 1999;26(10):836-40.
  • Hulsmann M, Rummelin C, Schafers F. Root canal cleanliness after preparation with different endodontic handpieces and hand instruments: a comparative SEM investigation. J Endod 1997;23(5):301-6.
  • Sharma V, Rampal P, Kumar S. Shear bond strength of composite resin to dentin after application of cavity disinfectants - SEM study. Contemp Clin Dent 2011;2(3):155-9.
  • Martin FE, Nadkarni MA, Jacques NA, Hunter N. Quantitative microbiological study of human carious dentine by culture and real-time PCR: association of anaerobes with histopathological changes in chronic pulpitis. J Clin Microbiol 2002;40(5):1698- 704.
  • Arita M, Nagayoshi M, Fukuizumi T, et al. Microbicidal efficacy of ozonated water against Candida albicans adhering to acrylic denture plates. Oral Microbiol Immunol 2005;20(4):206-10.
  • Baysan A, Whiley RA, Lynch E. Antimicrobial effect of a novel ozone- generating device on micro-organisms associated with primary 2000;34(6):498-501. in vitro. Caries Res
  • Knight GM, McIntyre JM, Craig GG, Mulyani, Zilm PS. The inability of Streptococcus mutans and Lactobacillus acidophilus to form a biofilm in vitro on dentine pretreated with ozone. Aust Dent J 2008;53(4):349-53.
  • Gursoy H, Cakar G, Ipci SD, Kuru B, Yilmaz S. In vitro evaluation of the effects of different treatment procedures on dentine tubules. Photomed Laser Surg 2012;30(12):695-8.
  • Renton-Harper P, Midda M. NdYAG laser treatment of dentinal hypersensitivity. Br Dent J 1992;172(1):13-6.
  • Zhang S, Chen T, Ge LH. Scanning electron microscopy study of cavity preparation in deciduous teeth using the Er:YAG laser with different powers. Lasers Med Sci 2012;27(1):141-4.
  • Kalyoncuoglu E, Demiryurek EO. A Comparative Scanning Electron Microscopy Evaluation of Smear Layer Removal from Teeth with Different Irrigation Solutions and Lasers. Microsc Microanal 2013:1-5.
  • Zhang S, Chen T, Ge LH. [Scanning electron microscopy was used to observe dentin morphology in primary and permanent teeth treated by erbium: yttrium-aluminum-garnet laser]. Beijing Da Xue Xue Bao 2011;43(5):766-9.
  • Soares LE, Martin OC, Moriyama LT, Kurachi C, Martin AA. Relationship between the chemical and morphological characteristics of human dentin after Er:YAG laser irradiation. J Biomed Opt 2013;18(6):068001.
  • Stabholz A, Sahar-Helft S, Moshonov J. Lasers in endodontics. Dent Clin North Am 2004;48(4):809-32, vi.
  • Dalkilic EE, Arisu HD, Kivanc BH, Uctasli MB, Omurlu H. Effect of different disinfectant methods on the initial microtensile bond strength of a self-etch adhesive to dentin. Lasers Med Sci 2012;27(4):819-25.

Scanning Electron Microscopic Analyses of the Effects of Different Disinfectant Methods on Dentinal Structure

Year 2013, Volume: 6 Issue: 2, 65 - 68, 01.06.2013

Abstract

References

  • Meiers JC, Kresin JC. Cavity disinfectants and dentin bonding. Oper Dent 1996;21(4):153-9.
  • Ersin NK, Candan U, Aykut A, Eronat C, Belli S. No adverse effect to bonding following caries disinfection with chlorhexidine. J Dent Child (Chic) 2009;76(1):20-7.
  • Brannstr.M, Johnson G. Effects of Various Conditioners and Cleaning Agents on Prepared Dentin Surfaces - Scanning Electron-Microscopic Investigation. Journal of Prosthetic Dentistry 1974;31(4):422-30.
  • Schultz RJ, Harvey GP, Fernandezberos ME, et al. Bactericidal Effects of the Neodymium Yag Laser - Invitro Study. Lasers in Surgery and Medicine 1986;6(5):445-48.
  • Yamayoshi T, Tatsumi N. Microbicidal effects of ozone solution on methicillin-resistant Staphylococcus aureus. Drugs Exp Clin Res 1993;19(2):59-64.
  • Bocci V, Luzzi E, Corradeschi F, Paulesu L, Distefano A. Studies on the Biological Effects of Ozone .3. An Attempt to Define Conditions for Optimal Induction of Cytokines. Lymphokine and Cytokine Research 1993;12(2):121-26.
  • Gurgan S, Bolay S, Kiremitci A. Effect of disinfectant application methods on the bond strength of composite to dentin. Journal of Oral Rehabilitation 1999;26(10):836-40.
  • Hulsmann M, Rummelin C, Schafers F. Root canal cleanliness after preparation with different endodontic handpieces and hand instruments: a comparative SEM investigation. J Endod 1997;23(5):301-6.
  • Sharma V, Rampal P, Kumar S. Shear bond strength of composite resin to dentin after application of cavity disinfectants - SEM study. Contemp Clin Dent 2011;2(3):155-9.
  • Martin FE, Nadkarni MA, Jacques NA, Hunter N. Quantitative microbiological study of human carious dentine by culture and real-time PCR: association of anaerobes with histopathological changes in chronic pulpitis. J Clin Microbiol 2002;40(5):1698- 704.
  • Arita M, Nagayoshi M, Fukuizumi T, et al. Microbicidal efficacy of ozonated water against Candida albicans adhering to acrylic denture plates. Oral Microbiol Immunol 2005;20(4):206-10.
  • Baysan A, Whiley RA, Lynch E. Antimicrobial effect of a novel ozone- generating device on micro-organisms associated with primary 2000;34(6):498-501. in vitro. Caries Res
  • Knight GM, McIntyre JM, Craig GG, Mulyani, Zilm PS. The inability of Streptococcus mutans and Lactobacillus acidophilus to form a biofilm in vitro on dentine pretreated with ozone. Aust Dent J 2008;53(4):349-53.
  • Gursoy H, Cakar G, Ipci SD, Kuru B, Yilmaz S. In vitro evaluation of the effects of different treatment procedures on dentine tubules. Photomed Laser Surg 2012;30(12):695-8.
  • Renton-Harper P, Midda M. NdYAG laser treatment of dentinal hypersensitivity. Br Dent J 1992;172(1):13-6.
  • Zhang S, Chen T, Ge LH. Scanning electron microscopy study of cavity preparation in deciduous teeth using the Er:YAG laser with different powers. Lasers Med Sci 2012;27(1):141-4.
  • Kalyoncuoglu E, Demiryurek EO. A Comparative Scanning Electron Microscopy Evaluation of Smear Layer Removal from Teeth with Different Irrigation Solutions and Lasers. Microsc Microanal 2013:1-5.
  • Zhang S, Chen T, Ge LH. [Scanning electron microscopy was used to observe dentin morphology in primary and permanent teeth treated by erbium: yttrium-aluminum-garnet laser]. Beijing Da Xue Xue Bao 2011;43(5):766-9.
  • Soares LE, Martin OC, Moriyama LT, Kurachi C, Martin AA. Relationship between the chemical and morphological characteristics of human dentin after Er:YAG laser irradiation. J Biomed Opt 2013;18(6):068001.
  • Stabholz A, Sahar-Helft S, Moshonov J. Lasers in endodontics. Dent Clin North Am 2004;48(4):809-32, vi.
  • Dalkilic EE, Arisu HD, Kivanc BH, Uctasli MB, Omurlu H. Effect of different disinfectant methods on the initial microtensile bond strength of a self-etch adhesive to dentin. Lasers Med Sci 2012;27(4):819-25.
There are 21 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Taskin Gurbuz This is me

Fatih Sengul This is me

Tevfik Demirci This is me

Munevver Coruh This is me

Publication Date June 1, 2013
Published in Issue Year 2013 Volume: 6 Issue: 2

Cite

APA Gurbuz, T., Sengul, F., Demirci, T., Coruh, M. (2013). Scanning Electron Microscopic Analyses of the Effects of Different Disinfectant Methods on Dentinal Structure. Journal Of International Dental And Medical Research, 6(2), 65-68.
AMA Gurbuz T, Sengul F, Demirci T, Coruh M. Scanning Electron Microscopic Analyses of the Effects of Different Disinfectant Methods on Dentinal Structure. JIDMR. June 2013;6(2):65-68.
Chicago Gurbuz, Taskin, Fatih Sengul, Tevfik Demirci, and Munevver Coruh. “Scanning Electron Microscopic Analyses of the Effects of Different Disinfectant Methods on Dentinal Structure”. Journal Of International Dental And Medical Research 6, no. 2 (June 2013): 65-68.
EndNote Gurbuz T, Sengul F, Demirci T, Coruh M (June 1, 2013) Scanning Electron Microscopic Analyses of the Effects of Different Disinfectant Methods on Dentinal Structure. Journal Of International Dental And Medical Research 6 2 65–68.
IEEE T. Gurbuz, F. Sengul, T. Demirci, and M. Coruh, “Scanning Electron Microscopic Analyses of the Effects of Different Disinfectant Methods on Dentinal Structure”, JIDMR, vol. 6, no. 2, pp. 65–68, 2013.
ISNAD Gurbuz, Taskin et al. “Scanning Electron Microscopic Analyses of the Effects of Different Disinfectant Methods on Dentinal Structure”. Journal Of International Dental And Medical Research 6/2 (June 2013), 65-68.
JAMA Gurbuz T, Sengul F, Demirci T, Coruh M. Scanning Electron Microscopic Analyses of the Effects of Different Disinfectant Methods on Dentinal Structure. JIDMR. 2013;6:65–68.
MLA Gurbuz, Taskin et al. “Scanning Electron Microscopic Analyses of the Effects of Different Disinfectant Methods on Dentinal Structure”. Journal Of International Dental And Medical Research, vol. 6, no. 2, 2013, pp. 65-68.
Vancouver Gurbuz T, Sengul F, Demirci T, Coruh M. Scanning Electron Microscopic Analyses of the Effects of Different Disinfectant Methods on Dentinal Structure. JIDMR. 2013;6(2):65-8.