Cerebrospinal Fluid Analysis of Pericytic Mediators in Clinically Isolated Syndrome and Multiple Sclerosis: A Preliminary Study

Year 2018, Volume: 8 Issue: 1, 18 - 22, 30.04.2018

Tuncay Gündüz , Tuba Tanyel Kiremitçi Canan Ulusoy Murat Kürtüncü Recai Türkoğlu

Abstract

DOI: 10.26650/experimed.2018.434227

Objectives: In many studies, blood brain
barrier has been shown to be compromised in multiple sclerosis patients.
Pericytes play an active role in ensuring the continuity of the blood brain
barrier along with a series of cells. In this study, the effect of pericytic
dysfunction on the development of demyelinating plaques in patients with
multiple sclerosis was investigated.

Material and Method: Concentrations of
pericyte dysfunction mediators (PDGFbb, MMP9, TIMP3 and ADAM17) in
cerebrospinal fluid of patients with clinically isolated syndrome (CIS),
relapsing remitting multiple sclerosis (RRMS) and healthy control group were
measured by ELISA and oligoclonal bands (OCB) were investigated. We aimed to
determine whether the concentration of these mediators differed between groups
and whether they correlated with lesion load, number of attacks, and EDSS
scores.

Results: Concentrations of all four
mediators were similar in patients with CIS and RRMS. However, both groups were
found to be higher than the healthy group. In the CIS and RRMS groups, the
levels of the mediators were not correlated with any parameters examined.
However, the levels of PDGFbb (p=0.045), MMP9 (p=0.037), and TIMP3 (p=0.033)
were higher in OCB positive patients than in those without OCB, whereas ADAM17
levels remained unchanged.

Conclusion: This study shows that pericytes
may play a role in the pathogenesis of MS from early stages of the disease. The
presence of higher levels in patients with OCB suggests that pericyte
dysfunction may be associated with OCB formation.

Keywords

clinically isolated syndrome, pericyte, cerebrospinal fluid, neuroinflammation, Multiple sclerosis

Klinik İzole Sendrom ve Multipl Skleroz Hastalarının Beyin Omurilik Sıvılarında Perisitik Mediatörlerin Analizi: Pilot Çalışma

Abstract

DOI: 10.26650/experimed.2018.434227

Amaç: Multipl skleroz’da kan beyin
bariyerinin bozulduğu pek çok çalışmada gösterilmiştir. Perisitler bir dizi
hücre yanında kan beyin bariyerinin devamlılığının sağlanmasında aktif rol
üstlenir. Bu çalışmada multipl skleroz hastalarında perisit disfonksiyonunun
demyelinizan plakların gelişimindeki etkisi araştırılmıştır.

Gereç ve Yöntem: Klinik izole sendrom
(KİS), relapsing remitting multipl skleroz (RRMS) ve sağlıklı kontrol grubundan
alınan beyin omurilik sıvılarında perisit disfonksiyon mediatörlerinin (PDGFbb,
MMP9, TIMP3 ve ADAM17) konsantrasyonları ELISA ile ölçüldü ve oligoklonal
bantlar (OKB) araştırıldı. Bu mediyatörlerin, gruplar arasındaki farklılıkları
lezyon yükü, atak sayısı ve EDSS skorları ile korelasyon gösterip göstermediği
belirlendi.

Bulgular: Her dört mediyatörün
konsantrasyonları KİS ve RRMS hastalarında benzer bulundu. Bununla beraber her
iki grupta sağlıklı kontollerden yüksek bulundu. KİS ve RRMS grubunda,
mediyatörlerin seviyelerinin bakılan hiçbir parametre ile korelasyon göstermediği
saptandı. Buna karşın, beyin omurilik sıvılarında OKB bulunan hastalarda PDGFbb
(p=0,045), MMP9 (p=0,037) ve TIMP3 (p=0,033) düzeylerinin OKB bulunmayanlara
göre daha yüksek olduğu, ADAM17 seviyelerinin ise değişmediği görüldü.

Sonuç: Bu çalışma perisit disfonksiyon
mediyatörlerinin MS’in patogenezinde hastalığın erken dönemlerinden itibaren
rol alabileceğini göstermektedir. Oligoklonal band bulunan hastalarda daha
yüksek seviyelerdeki mevcudiyeti, perisit disfonksiyonunun OKB oluşumu ile bir
ilgisi olabileceğini akla getirmektedir.

Keywords

klinik izole sendrom, perisit, beyin omurilik sıvısı, nöroinflamasyon, Multipl skleroz, nöroinflamasyon

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