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Chemical Analysis and Antimicrobial Effect of Propolis from Hakkari Province of Turkey against Some Pathogenic Microorganisms

Year 2017, Volume 76, Issue 2, 74 - 78, 27.12.2017
https://doi.org/10.5152/EurJBiol.2017.1713

Abstract

This study was performed to investigate the antimicrobial effect of propolis from Hakkari province of Turkey and its chemical content by Gas Chromatography–Mass Spectroscopy. In vitro inhibitory activity of propolis was studied by the disc diffusion method against six gram positive, three gram negative bacteria and one yeast like fungi. As a result of chemical analysis, the total flavonoid ratio of the propolis sample was found to be higher than the other compound groups. Pinostrobin chalcone, pinocembrin and chrysin were identified as major flavonoids. Also, all microorganisms tested were susceptible to the propolis extract except for Klebsiella pneumoniae. Minimum inhibition concentration (MIC) values were determined by microbroth dilution assay. MIC values against microorganisms ranged from 25 to 200 µg/mL. Antimicrobial susceptibility test results showed that inhibitory effect our propolis sample was somewhat weaker than ampicillin, but it had a broader spectrum.

References

  • 1. Kourkoutas Y, Chorianopoulos N, Karatzas KAG, Banat IM. Bioactive Natural Products 2016. Biomed Res Int 2016; 1-2. 2. Possamai MM, Honorio-Franca AC, Reinaque APB, França EL, Souto PC. Brazilian propolis: a natural product that improved the fungicidal activity by blood phagocytes. Biomed Res Int 2013; 2013: 541018. 3. Falcão SL, Vale N, Cos P, Gomes P, Freire C, Maes L, Vilas-Boas M. In vitro evaluation of portuguese propolis and floral sources for antiprotozoal, antibacterial and antifungal activity. Phytother Res 2014; 28(3): 437-43. 4. de Groot AC. Propolis: a review of properties, applications, chemical composition, contact allergy, and other adverse effects. Dermatitis 2013; 24(6): 263-82. 5. Madrigal-Santillán E, Madrigal-Bujaidar E, Álvarez-González I, Sumaya-Martínez MT, Gutiérrez-Salinas J, Bautista M, et al. Review of natural products with hepatoprotective effects. World J Gastroenterol 2014; 20(40): 14787-804. 6. Keskin N, Hazir S, Baser KHC, Kurkcuoglu M. Antibacterial activity and chemical composition of Turkish propolis. Z. Naturforsch C 2001; 56 (11-12): 1112-5. 7. Koru O, Toksoy F, Acikel CH, Tunca YM, Baysallar M, Guclu AU, et al. In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. Anaerobe 2007; 13(3): 140-5. 8. Kurek-Górecka A, Rzepecka-Stojko A, Górecki M, Stojko J, Sosada M, Świerczek-Zięba G. Structure and antioxidant activity of polyphenols derived from propolis. Molecules 2013; 19(1): 78-101. 9. Bayram S, Ecem Bayram N, Gerçek YC, Öz GC, Sorkun K. Anticytotoxic and antimutagenic effects of propolis on human lymphocytes in vitro. Mellifera 2016; 16 (2): 38-46. 10. Woo CG, Kang JS, Kim HJ, Kim YJ, Han B. Treatment of air filters using the antimicrobial natural products propolis and grapefruit seed extract for deactivation of bioaerosols. Aerosol Sci Technol 2015; 49(8): 611-9. 11. Türkez H, Geyikoğlu F, Yousef MI, Toğar B, Vançelik S. Propolis alleviates 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin-induced histological changes, oxidative stress and DNA damage in rat liver. Toxicol Ind Health 2013; 29(8): 677-85. 12. Ecem Bayram N, Karadayı M, Güllüce M, Bayram S, Sorkun K, Cevahir Öz G, et al. Genotoxic and antigenotoxic evaluation of propolis by using in vitro bacterial assay systems. Mellifera 2015; 15(1): 29-36. 13. Seda Vatansever H, Sorkun K, Ismet Deliloglu Gurhan S, Ozdal-Kurt F, Turkoz E, Gencay O, et al. Propolis from Turkey induces apoptosis through activating caspases in human breast carcinoma cell lines. Acta Histochem 2010; 112(6): 546-56. 14. Uzel A, Sorkun K, Oncag O, Çogulu D, Gencay O, Salih B. Chemical compositions and antimicrobial activities of four different Anatolian propolis samples. Microbiol Res 2005; 160(2): 189-95. 15. Kartal M, Yildiz S, Kaya S, Kurucu S, Topcu G. Antimicrobial activity of propolis samples from two different regions of Anatolia. J Ethnopharmacol 2003; 86(1): 69-73. 16. Silici S, Kutluca S. Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region. J Ethnopharmacol 2005; 99(1): 69-73. 17. Adıgüzel A, Hakan Ö, Münevver S, Medine G, Atalay S, Hamdullah K, et al. Antimicrobial and antioxidant activity of the essential oil and methanol extract of Nepeta cataria. PJM 2009; 58(1): 69-76. 18. Kalogeropoulos N, Konteles SJ, Troullidou E, Mourtzinos I, Karathanos VT. Chemical composition, antioxidant activity and antimicrobial properties of propolis extracts from Greece and Cyprus. Food Chem 2009; 116(2): 452-61. 19. Burdock GA. Review of the biological properties and toxicity of bee propolis (propolis). Food Chem Toxicol 1998; 36: 347-63. 20. Benhanifia M, Mohamed WM, Bellik Y, Benbarek H. Antimicrobial and antioxidant activities of different propolis samples from north-western Algeria. Int J Food Sci Technol 2013; 48(12): 2521-7. 21. Cushnie TT, Lamb AJ. Recent advances in understanding the antibacterial properties of flavonoids. Int J Antimicrob Agents 2011; 38(2): 99-107. 22. Procházková D, Boušová I, Wilhelmová N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia 2011; 82(4): 513-23. 23. Daglia M. Polyphenols as antimicrobial agents. Curr Opin Biotechnol 2012; 23(2): 174-81. 24. Gencay-Celemli O. Chemical classification of propolis samples collected from different regions of Turkey in geographical region base. Hacettepe J of Bio and Chem 2015; 45(1): 49-57. 25. Velikova M, Bankova V, Sorkun K, Popov S. Kujumgiev A. Turkiye ve Bulgaristan kokenli propolis orneklerinin kimyasal bilesimi ve biyolojik aktiviteleri. Mellifera 2001; 1(1): 24-6. 26. Sahinler N, Kaftanoglu O. Natural product propolis: chemical composition. Nat Prod Res 2005; 19(2): 183-8. 27. Stepanović S, Antić N, Dakić I, Švabić-Vlahović M. In vitro antimicrobial activity of propolis and synergism between propolis and antimicrobial drugs. Microbiol Res 2003; 158(4): 353-7. 28. Mirzoeva OK, Grishanin RN, Calder PC. Antimicrobial action of propolis and some of its components: the effects on growth, membrane potential and motility of bacteria. Microbiol Res 1997; 152: 239-46. 29. Drago L, Mombelli B, De Vecchi E, Fassina MC, Tocalli L, Gismondo MR. In vitro antimicrobial activity of propolis dry extract. J Chemother 2000; 12: 390-5. 30. Daugsch A, Moraes CS, Fort P, Park YK. Brazilian red propolis-chemical composition and botanical origin. J Evid Based Complementary Alternat Med 2008; 5(4): 435-41. 31. Runyoro DK, Ngassapa OD, Kamugisha A. Antimicrobial activity of propolis from Tabora and Iringa Regions, Tanzania and synergism with gentamicin. J Appl Pharm Sci 2017; 7(1): 171-6. 32. Gao M, Zhang W, Liu Q, Hu J, Liu G, Du G. Pinocembrin prevents glutamate-induced apoptosis in SH-SY5Y neuronal cells via decrease of bax/bcl-2 ratio. Eur J Pharmacol 2008; 591(1-3): 73-9.

Year 2017, Volume 76, Issue 2, 74 - 78, 27.12.2017
https://doi.org/10.5152/EurJBiol.2017.1713

Abstract

References

  • 1. Kourkoutas Y, Chorianopoulos N, Karatzas KAG, Banat IM. Bioactive Natural Products 2016. Biomed Res Int 2016; 1-2. 2. Possamai MM, Honorio-Franca AC, Reinaque APB, França EL, Souto PC. Brazilian propolis: a natural product that improved the fungicidal activity by blood phagocytes. Biomed Res Int 2013; 2013: 541018. 3. Falcão SL, Vale N, Cos P, Gomes P, Freire C, Maes L, Vilas-Boas M. In vitro evaluation of portuguese propolis and floral sources for antiprotozoal, antibacterial and antifungal activity. Phytother Res 2014; 28(3): 437-43. 4. de Groot AC. Propolis: a review of properties, applications, chemical composition, contact allergy, and other adverse effects. Dermatitis 2013; 24(6): 263-82. 5. Madrigal-Santillán E, Madrigal-Bujaidar E, Álvarez-González I, Sumaya-Martínez MT, Gutiérrez-Salinas J, Bautista M, et al. Review of natural products with hepatoprotective effects. World J Gastroenterol 2014; 20(40): 14787-804. 6. Keskin N, Hazir S, Baser KHC, Kurkcuoglu M. Antibacterial activity and chemical composition of Turkish propolis. Z. Naturforsch C 2001; 56 (11-12): 1112-5. 7. Koru O, Toksoy F, Acikel CH, Tunca YM, Baysallar M, Guclu AU, et al. In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. Anaerobe 2007; 13(3): 140-5. 8. Kurek-Górecka A, Rzepecka-Stojko A, Górecki M, Stojko J, Sosada M, Świerczek-Zięba G. Structure and antioxidant activity of polyphenols derived from propolis. Molecules 2013; 19(1): 78-101. 9. Bayram S, Ecem Bayram N, Gerçek YC, Öz GC, Sorkun K. Anticytotoxic and antimutagenic effects of propolis on human lymphocytes in vitro. Mellifera 2016; 16 (2): 38-46. 10. Woo CG, Kang JS, Kim HJ, Kim YJ, Han B. Treatment of air filters using the antimicrobial natural products propolis and grapefruit seed extract for deactivation of bioaerosols. Aerosol Sci Technol 2015; 49(8): 611-9. 11. Türkez H, Geyikoğlu F, Yousef MI, Toğar B, Vançelik S. Propolis alleviates 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin-induced histological changes, oxidative stress and DNA damage in rat liver. Toxicol Ind Health 2013; 29(8): 677-85. 12. Ecem Bayram N, Karadayı M, Güllüce M, Bayram S, Sorkun K, Cevahir Öz G, et al. Genotoxic and antigenotoxic evaluation of propolis by using in vitro bacterial assay systems. Mellifera 2015; 15(1): 29-36. 13. Seda Vatansever H, Sorkun K, Ismet Deliloglu Gurhan S, Ozdal-Kurt F, Turkoz E, Gencay O, et al. Propolis from Turkey induces apoptosis through activating caspases in human breast carcinoma cell lines. Acta Histochem 2010; 112(6): 546-56. 14. Uzel A, Sorkun K, Oncag O, Çogulu D, Gencay O, Salih B. Chemical compositions and antimicrobial activities of four different Anatolian propolis samples. Microbiol Res 2005; 160(2): 189-95. 15. Kartal M, Yildiz S, Kaya S, Kurucu S, Topcu G. Antimicrobial activity of propolis samples from two different regions of Anatolia. J Ethnopharmacol 2003; 86(1): 69-73. 16. Silici S, Kutluca S. Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region. J Ethnopharmacol 2005; 99(1): 69-73. 17. Adıgüzel A, Hakan Ö, Münevver S, Medine G, Atalay S, Hamdullah K, et al. Antimicrobial and antioxidant activity of the essential oil and methanol extract of Nepeta cataria. PJM 2009; 58(1): 69-76. 18. Kalogeropoulos N, Konteles SJ, Troullidou E, Mourtzinos I, Karathanos VT. Chemical composition, antioxidant activity and antimicrobial properties of propolis extracts from Greece and Cyprus. Food Chem 2009; 116(2): 452-61. 19. Burdock GA. Review of the biological properties and toxicity of bee propolis (propolis). Food Chem Toxicol 1998; 36: 347-63. 20. Benhanifia M, Mohamed WM, Bellik Y, Benbarek H. Antimicrobial and antioxidant activities of different propolis samples from north-western Algeria. Int J Food Sci Technol 2013; 48(12): 2521-7. 21. Cushnie TT, Lamb AJ. Recent advances in understanding the antibacterial properties of flavonoids. Int J Antimicrob Agents 2011; 38(2): 99-107. 22. Procházková D, Boušová I, Wilhelmová N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia 2011; 82(4): 513-23. 23. Daglia M. Polyphenols as antimicrobial agents. Curr Opin Biotechnol 2012; 23(2): 174-81. 24. Gencay-Celemli O. Chemical classification of propolis samples collected from different regions of Turkey in geographical region base. Hacettepe J of Bio and Chem 2015; 45(1): 49-57. 25. Velikova M, Bankova V, Sorkun K, Popov S. Kujumgiev A. Turkiye ve Bulgaristan kokenli propolis orneklerinin kimyasal bilesimi ve biyolojik aktiviteleri. Mellifera 2001; 1(1): 24-6. 26. Sahinler N, Kaftanoglu O. Natural product propolis: chemical composition. Nat Prod Res 2005; 19(2): 183-8. 27. Stepanović S, Antić N, Dakić I, Švabić-Vlahović M. In vitro antimicrobial activity of propolis and synergism between propolis and antimicrobial drugs. Microbiol Res 2003; 158(4): 353-7. 28. Mirzoeva OK, Grishanin RN, Calder PC. Antimicrobial action of propolis and some of its components: the effects on growth, membrane potential and motility of bacteria. Microbiol Res 1997; 152: 239-46. 29. Drago L, Mombelli B, De Vecchi E, Fassina MC, Tocalli L, Gismondo MR. In vitro antimicrobial activity of propolis dry extract. J Chemother 2000; 12: 390-5. 30. Daugsch A, Moraes CS, Fort P, Park YK. Brazilian red propolis-chemical composition and botanical origin. J Evid Based Complementary Alternat Med 2008; 5(4): 435-41. 31. Runyoro DK, Ngassapa OD, Kamugisha A. Antimicrobial activity of propolis from Tabora and Iringa Regions, Tanzania and synergism with gentamicin. J Appl Pharm Sci 2017; 7(1): 171-6. 32. Gao M, Zhang W, Liu Q, Hu J, Liu G, Du G. Pinocembrin prevents glutamate-induced apoptosis in SH-SY5Y neuronal cells via decrease of bax/bcl-2 ratio. Eur J Pharmacol 2008; 591(1-3): 73-9.

Details

Subjects Science
Journal Section Research Articles
Authors

Sinan BAYRAM This is me
Bayburt University, Vocational School of Health Services, Department of Medical Services and Techniques, Bayburt, Turkey


Nesrin Ecem BAYRAM> (Primary Author)
Bayburt University, Aydıntepe Vocational College, Department of Food Processing, Bayburt, Turkey; Bayburt University, Beekeeping Research, Development and Application Center, Bayburt, Turkey
0000-0002-5496-8194


Yusuf Can GERCEK This is me
Istanbul University, Faculty of Sciences, Department of Biology, Istanbul, Turkey


Mehmet Nuri AYDOGAN This is me
Ataturk University, Faculty of Sciences, Department of Biology, Erzurum, Turkey


Gul CEVAHİR OZ This is me
Istanbul University, Faculty of Sciences, Department of Biology, Istanbul, Turkey

Publication Date December 27, 2017
Application Date July 25, 2017
Acceptance Date
Published in Issue Year 2017, Volume 76, Issue 2

Cite

Bibtex @research article { iufsjb381705, journal = {European Journal of Biology}, issn = {2602-2575}, eissn = {2618-6144}, address = {}, publisher = {Istanbul University}, year = {2017}, volume = {76}, number = {2}, pages = {74 - 78}, doi = {10.5152/EurJBiol.2017.1713}, title = {Chemical Analysis and Antimicrobial Effect of Propolis from Hakkari Province of Turkey against Some Pathogenic Microorganisms}, key = {cite}, author = {Bayram, Sinan and Bayram, Nesrin Ecem and Gercek, Yusuf Can and Aydogan, Mehmet Nuri and Cevahir Oz, Gul} }
APA Bayram, S. , Bayram, N. E. , Gercek, Y. C. , Aydogan, M. N. & Cevahir Oz, G. (2017). Chemical Analysis and Antimicrobial Effect of Propolis from Hakkari Province of Turkey against Some Pathogenic Microorganisms . European Journal of Biology , 76 (2) , 74-78 . DOI: 10.5152/EurJBiol.2017.1713
MLA Bayram, S. , Bayram, N. E. , Gercek, Y. C. , Aydogan, M. N. , Cevahir Oz, G. "Chemical Analysis and Antimicrobial Effect of Propolis from Hakkari Province of Turkey against Some Pathogenic Microorganisms" . European Journal of Biology 76 (2017 ): 74-78 <https://dergipark.org.tr/en/pub/iufsjb/issue/34550/381705>
Chicago Bayram, S. , Bayram, N. E. , Gercek, Y. C. , Aydogan, M. N. , Cevahir Oz, G. "Chemical Analysis and Antimicrobial Effect of Propolis from Hakkari Province of Turkey against Some Pathogenic Microorganisms". European Journal of Biology 76 (2017 ): 74-78
RIS TY - JOUR T1 - Chemical Analysis and Antimicrobial Effect of Propolis from Hakkari Province of Turkey against Some Pathogenic Microorganisms AU - SinanBayram, Nesrin EcemBayram, Yusuf CanGercek, Mehmet NuriAydogan, GulCevahir Oz Y1 - 2017 PY - 2017 N1 - doi: 10.5152/EurJBiol.2017.1713 DO - 10.5152/EurJBiol.2017.1713 T2 - European Journal of Biology JF - Journal JO - JOR SP - 74 EP - 78 VL - 76 IS - 2 SN - 2602-2575-2618-6144 M3 - doi: 10.5152/EurJBiol.2017.1713 UR - https://doi.org/10.5152/EurJBiol.2017.1713 Y2 - 2022 ER -
EndNote %0 European Journal of Biology Chemical Analysis and Antimicrobial Effect of Propolis from Hakkari Province of Turkey against Some Pathogenic Microorganisms %A Sinan Bayram , Nesrin Ecem Bayram , Yusuf Can Gercek , Mehmet Nuri Aydogan , Gul Cevahir Oz %T Chemical Analysis and Antimicrobial Effect of Propolis from Hakkari Province of Turkey against Some Pathogenic Microorganisms %D 2017 %J European Journal of Biology %P 2602-2575-2618-6144 %V 76 %N 2 %R doi: 10.5152/EurJBiol.2017.1713 %U 10.5152/EurJBiol.2017.1713
ISNAD Bayram, Sinan , Bayram, Nesrin Ecem , Gercek, Yusuf Can , Aydogan, Mehmet Nuri , Cevahir Oz, Gul . "Chemical Analysis and Antimicrobial Effect of Propolis from Hakkari Province of Turkey against Some Pathogenic Microorganisms". European Journal of Biology 76 / 2 (December 2017): 74-78 . https://doi.org/10.5152/EurJBiol.2017.1713
AMA Bayram S. , Bayram N. E. , Gercek Y. C. , Aydogan M. N. , Cevahir Oz G. Chemical Analysis and Antimicrobial Effect of Propolis from Hakkari Province of Turkey against Some Pathogenic Microorganisms. Eur J Biol. 2017; 76(2): 74-78.
Vancouver Bayram S. , Bayram N. E. , Gercek Y. C. , Aydogan M. N. , Cevahir Oz G. Chemical Analysis and Antimicrobial Effect of Propolis from Hakkari Province of Turkey against Some Pathogenic Microorganisms. European Journal of Biology. 2017; 76(2): 74-78.
IEEE S. Bayram , N. E. Bayram , Y. C. Gercek , M. N. Aydogan and G. Cevahir Oz , "Chemical Analysis and Antimicrobial Effect of Propolis from Hakkari Province of Turkey against Some Pathogenic Microorganisms", European Journal of Biology, vol. 76, no. 2, pp. 74-78, Dec. 2017, doi:10.5152/EurJBiol.2017.1713