Chemical Analysis and Antimicrobial Effect of Propolis from Hakkari Province of Turkey against Some Pathogenic Microorganisms

Year 2017, Volume: 76 Issue: 2, 74 - 78, 27.12.2017

Sinan Bayram Nesrin Ecem Bayram , Yusuf Can Gercek Mehmet Nuri Aydogan Gul Cevahir Oz

https://doi.org/10.5152/EurJBiol.2017.1713

Abstract

This
study was performed to investigate the antimicrobial effect of propolis from
Hakkari province of Turkey and its chemical content by Gas Chromatography–Mass
Spectroscopy. In vitro inhibitory activity of propolis was studied by the disc
diffusion method against six gram positive, three gram negative bacteria and
one yeast like fungi. As a result of chemical analysis, the total flavonoid
ratio of the propolis sample was found to be higher than the other compound
groups. Pinostrobin chalcone, pinocembrin and chrysin were identified as major
flavonoids. Also, all microorganisms tested were susceptible to the propolis
extract except for Klebsiella pneumoniae. Minimum inhibition concentration
(MIC) values were determined by microbroth dilution assay. MIC values against
microorganisms ranged from 25 to 200 µg/mL. Antimicrobial susceptibility test
results showed that inhibitory effect our propolis sample was somewhat weaker
than ampicillin, but it had a broader spectrum.

Keywords

Ampicillin, antimicrobial effect, minimum inhibition concentration (MIC), propolis content

References

• 1. Kourkoutas Y, Chorianopoulos N, Karatzas KAG, Banat IM. Bioactive Natural Products 2016. Biomed Res Int 2016; 1-2. 2. Possamai MM, Honorio-Franca AC, Reinaque APB, França EL, Souto PC. Brazilian propolis: a natural product that improved the fungicidal activity by blood phagocytes. Biomed Res Int 2013; 2013: 541018. 3. Falcão SL, Vale N, Cos P, Gomes P, Freire C, Maes L, Vilas-Boas M. In vitro evaluation of portuguese propolis and floral sources for antiprotozoal, antibacterial and antifungal activity. Phytother Res 2014; 28(3): 437-43. 4. de Groot AC. Propolis: a review of properties, applications, chemical composition, contact allergy, and other adverse effects. Dermatitis 2013; 24(6): 263-82. 5. Madrigal-Santillán E, Madrigal-Bujaidar E, Álvarez-González I, Sumaya-Martínez MT, Gutiérrez-Salinas J, Bautista M, et al. Review of natural products with hepatoprotective effects. World J Gastroenterol 2014; 20(40): 14787-804. 6. Keskin N, Hazir S, Baser KHC, Kurkcuoglu M. Antibacterial activity and chemical composition of Turkish propolis. Z. Naturforsch C 2001; 56 (11-12): 1112-5. 7. Koru O, Toksoy F, Acikel CH, Tunca YM, Baysallar M, Guclu AU, et al. In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. Anaerobe 2007; 13(3): 140-5. 8. Kurek-Górecka A, Rzepecka-Stojko A, Górecki M, Stojko J, Sosada M, Świerczek-Zięba G. Structure and antioxidant activity of polyphenols derived from propolis. Molecules 2013; 19(1): 78-101. 9. Bayram S, Ecem Bayram N, Gerçek YC, Öz GC, Sorkun K. Anticytotoxic and antimutagenic effects of propolis on human lymphocytes in vitro. Mellifera 2016; 16 (2): 38-46. 10. Woo CG, Kang JS, Kim HJ, Kim YJ, Han B. Treatment of air filters using the antimicrobial natural products propolis and grapefruit seed extract for deactivation of bioaerosols. Aerosol Sci Technol 2015; 49(8): 611-9. 11. Türkez H, Geyikoğlu F, Yousef MI, Toğar B, Vançelik S. Propolis alleviates 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin-induced histological changes, oxidative stress and DNA damage in rat liver. Toxicol Ind Health 2013; 29(8): 677-85. 12. Ecem Bayram N, Karadayı M, Güllüce M, Bayram S, Sorkun K, Cevahir Öz G, et al. Genotoxic and antigenotoxic evaluation of propolis by using in vitro bacterial assay systems. Mellifera 2015; 15(1): 29-36. 13. Seda Vatansever H, Sorkun K, Ismet Deliloglu Gurhan S, Ozdal-Kurt F, Turkoz E, Gencay O, et al. Propolis from Turkey induces apoptosis through activating caspases in human breast carcinoma cell lines. Acta Histochem 2010; 112(6): 546-56. 14. Uzel A, Sorkun K, Oncag O, Çogulu D, Gencay O, Salih B. Chemical compositions and antimicrobial activities of four different Anatolian propolis samples. Microbiol Res 2005; 160(2): 189-95. 15. Kartal M, Yildiz S, Kaya S, Kurucu S, Topcu G. Antimicrobial activity of propolis samples from two different regions of Anatolia. J Ethnopharmacol 2003; 86(1): 69-73. 16. Silici S, Kutluca S. Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region. J Ethnopharmacol 2005; 99(1): 69-73. 17. Adıgüzel A, Hakan Ö, Münevver S, Medine G, Atalay S, Hamdullah K, et al. Antimicrobial and antioxidant activity of the essential oil and methanol extract of Nepeta cataria. PJM 2009; 58(1): 69-76. 18. Kalogeropoulos N, Konteles SJ, Troullidou E, Mourtzinos I, Karathanos VT. Chemical composition, antioxidant activity and antimicrobial properties of propolis extracts from Greece and Cyprus. Food Chem 2009; 116(2): 452-61. 19. Burdock GA. Review of the biological properties and toxicity of bee propolis (propolis). Food Chem Toxicol 1998; 36: 347-63. 20. Benhanifia M, Mohamed WM, Bellik Y, Benbarek H. Antimicrobial and antioxidant activities of different propolis samples from north-western Algeria. Int J Food Sci Technol 2013; 48(12): 2521-7. 21. Cushnie TT, Lamb AJ. Recent advances in understanding the antibacterial properties of flavonoids. Int J Antimicrob Agents 2011; 38(2): 99-107. 22. Procházková D, Boušová I, Wilhelmová N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia 2011; 82(4): 513-23. 23. Daglia M. Polyphenols as antimicrobial agents. Curr Opin Biotechnol 2012; 23(2): 174-81. 24. Gencay-Celemli O. Chemical classification of propolis samples collected from different regions of Turkey in geographical region base. Hacettepe J of Bio and Chem 2015; 45(1): 49-57. 25. Velikova M, Bankova V, Sorkun K, Popov S. Kujumgiev A. Turkiye ve Bulgaristan kokenli propolis orneklerinin kimyasal bilesimi ve biyolojik aktiviteleri. Mellifera 2001; 1(1): 24-6. 26. Sahinler N, Kaftanoglu O. Natural product propolis: chemical composition. Nat Prod Res 2005; 19(2): 183-8. 27. Stepanović S, Antić N, Dakić I, Švabić-Vlahović M. In vitro antimicrobial activity of propolis and synergism between propolis and antimicrobial drugs. Microbiol Res 2003; 158(4): 353-7. 28. Mirzoeva OK, Grishanin RN, Calder PC. Antimicrobial action of propolis and some of its components: the effects on growth, membrane potential and motility of bacteria. Microbiol Res 1997; 152: 239-46. 29. Drago L, Mombelli B, De Vecchi E, Fassina MC, Tocalli L, Gismondo MR. In vitro antimicrobial activity of propolis dry extract. J Chemother 2000; 12: 390-5. 30. Daugsch A, Moraes CS, Fort P, Park YK. Brazilian red propolis-chemical composition and botanical origin. J Evid Based Complementary Alternat Med 2008; 5(4): 435-41. 31. Runyoro DK, Ngassapa OD, Kamugisha A. Antimicrobial activity of propolis from Tabora and Iringa Regions, Tanzania and synergism with gentamicin. J Appl Pharm Sci 2017; 7(1): 171-6. 32. Gao M, Zhang W, Liu Q, Hu J, Liu G, Du G. Pinocembrin prevents glutamate-induced apoptosis in SH-SY5Y neuronal cells via decrease of bax/bcl-2 ratio. Eur J Pharmacol 2008; 591(1-3): 73-9.