The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells

Year 2018, Volume: 77 Issue: 2, 81 - 88, 02.12.2018

Ozlem Dagdeviren Ozsoylemez Gul Ozcan

Abstract

DOI: 10.26650/EurJBiol.2018.0016

Objective: This study was conducted to
determine the antiproliferative effects of paclitaxel (PAC) and Colchicum
umbrosum plant extract on C-4 I and Vero cells. In addition, the apoptotic
effects of plant extract on these cells were compared.

Materials and Methods: C-4 I and Vero cells
were treated with PAC and corm extract of C. umbrosum for 24 h. The
antiproliferative effects of 0.01, 0.1, and 1 mg/mL of plant extract and 7.5,
15, and 30 nM of PAC and the half maximal inhibitory concentration (IC50)
values were determined by the MTT method. Morphological changes occurring in
the C-4 I and Vero cells were observed under phase-contrast and fluorescence
microscopes using DAPI staining.

Results: The IC50 values were found to be
0.01 mg/mL in C-4 I cells and 1 mg/mL in Vero cells for the plant extract and
15 nM in C-4 I and Vero cells for PAC. The apoptotic index (AI) values of the
experimental groups of C-4 I and Vero cells were significantly increased
compared to those of the control group (p<0.01) after treatment with IC50
concentration of the plant extract. The AI values of the plant extract
treatment in C-4 I cells were higher than those in Vero cells at 24 and 48 h.

Conclusion: Treatment with the IC50
concentration of the plant extract induced apoptotic cell death in C-4 I cells.
It can worth be considered as a novel promising candidate for the treatment of
cancer.

Keywords

C-4 I, Vero, Colchicum umbrosum, paclitaxel, cancer

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