DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE

Göknur Terzi; Ufuk Kamber; Cevat Nisbet; Sena Çenesiz

doi:10.16988/iuvfd.46753

DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE

Abstract

ABSTRACT

Vitamin B₁₂ is a member of the vitamin B complex. It contains cobalt, and is also known as cobalamin. It is exclusively synthesised by bacteria and is found primarily in fish, meat, poultry, eggs, milk and milk products. Vitamin B₁₂deficiency have an underlying stomach or intestinal disorder that limits the absorption of vitamin B₁₂ and might be caused to megaloblastic anemia. In this study cow milk samples were collected from in two different region in Turkey. In milk samples the amount of vitamin B₁₂ was detected by using ELISA. The vitamin B₁₂ concentrations of milk samples obtained from the cows of Holstein, Jersey, Zavot and East Anatolian Red species were determined as 0.98µg/L, 1.15µg/L, 2.93µg/L and 1.56µg/L, respectively. The concentrations of vitamin B₁₂ in Zavot cows milk were higher than the other species.

Key words: Vitamin B₁₂, cow milk, ELISA.

FARKLI SIĞIR IRKLARINA AİT SÜT ÖRNEKLERİNDE B 12 VİTAMİN DÜZEYİNİN ELİSA TEKNİĞİ İLE TESPİTİ

ÖZET

B₁₂ vitamini, B kompleks vitaminlerin bir üyesidir. Bu vitamin kobalt içerdiğinden dolayı kobalamin olarak adlandırılmaktadır. B₁₂ vitamini balık, et, tavuk, yumurta ve süt ürünlerinde bulunur ve bakteriler tarafından sentezlenir. B₁₂ vitamini yetersizliğine bağlı olarak mide ve bağırsak bozukluklar, kol ve bacaklarda uyuşukluk ve megolablastik anemi şekillenir. Bu çalışmada farklı sığır ırklarına ait süt örneklerinde B₁₂ vitamin düzeyleri araştırıldı. Bu amaçla Samsun ve Kars bölgesinden Holştayn, Jersey, Zavot and Doğu Anadolu Kırmızısı sığır ırklarına ait süt örnekleri toplandı. Süt örneklerinde B₁₂ vitamini miktarı ELISA yöntemi ile tespit edildi. B₁₂ vitamini Holştayn, Jersey, Zavot and Doğu Anadolu Kırmızısı sığır ırklarına ait süt örneklerinde sırasıyla 0.98µg/L, 1.15µg/L, 2.93µg/L ve 1.56µg/L olarak bulundu. Yapılan istatistik çalışması sonucunda vitamin B₁₂ miktarı Zavot ırkına ait süt örneklerinde diğer ırklardan daha yüksek bulundu (p<0.05, ANOVA).

Anahtar Kelimeler: B₁₂ vitamini, inek sütü, ELISA.

Keywords

B1 2 vitamini, inek sütü, ELISA

References

Alais, C.: Science du lait. Principes des techniques laitières (Ed. Sepaic) Paris, 1984.
Alpan, O.: The breeding and production of cattle. Ankara University Veterinary Faculty Zootechny Dept, Ankara, 1992.
Anonymous: http://www.tarim.gov.tr, 2007.
Bernard, M.A., Nakonezny, P.A., Kashner, T.M.: The effect of vitamin B1 2 deficiency on older veterans and its relationship to health. J. Am Geriatr Soc, 1998; 46: 1199- 1206.
Bestuzheva, K.T.: Chemical composition of camel milk and colostrum. Konevodstvoi Konnyi Sport, 1964; 34: 19.
Bottiglieri, T.: Folate, vitamin B12, and neuropsychiatric disorders. Nutr Rev, 1996; 54: 382- 390.
Combs, G.: Vitamin B12 in The Vitamins. Academic Press, New York, 1992.
Dagnelie, P.C., Van Staveren, W.A., Roos, A.H., Tuinstra, L.G. Burema, J:. Nutrients and contaminants in Human milk from mothers on macrobiotic and omnivorous diets. Eur J Clin Nutr, 1992; 46:355- 366.

Donangelo, C.M., Trugo, N.M., Koury, J.C., Barreto Silva, M.I., Freitas, L.A., Feldheim, W., Barth, C.: Iron, zinc, folate and vitamin B1 2 nutritional status and milk composition of low income Brazilian mothers. Eur J Clin Nutr, 1989; 43: 253- 266.
Food and Nutrition Board: Vitamin B12 Dietary Reference Intakes: Thiamin, Riboflavin, Niacin, Vitamin B- 6, Vitamin B12, Pantothenic Acid, Biotin, and Choline. National Academy Pres, Washington, 1998.
Ford, C., Rendle, M., Tracy, M., Richardson, V., Ford, H.: Vitamin B12 levels in human milk during the first nine months of lactation. Int J Vitam Nutr Res, 1996; 66: 329- 331.
Healton, E.B., Savage, D.G., Brust, J.C., Garrett, T.F., Lindenbaum, J.: Neurological aspects of cobalamin deficiency. Medicine, 1991; 70: 229- 244.
Herbert, V.: Vitamin B1 2 in Present Knowledge in Nutrition. 17th ed. International Life Sciences Institute Pres, Washington, 1996.
Mills, C.F.: Recent Advances in Animal Nutrition. In: U. HARESIGN, eds. Cobalt deficiency and cobalt requirements of ruminants. Butterworth, London. 129, 1981.
Monsen, A.L.B., Ueland, P.M.: Homocysteine and methylmalonic acid in diagnosis and risk assessment from infancy to adolescent. Am J Clin Nutr, 2003; 78: 7- 21.
Sawaya, W.N., Khalil, J.K., Al-Shalhat, A.F.: Mineral and vitamin content of goat's milk. J Am Diet Assoc, 1984; 84(4): 433- 435.
Shrimpton, D.H., Duckworth, J.: The vitamin-B12 (Cobalamin) content of the milk of lowland and hill sheep. J Sci Food Agr, 2006; 4(6): 301- 304.
Smith R, Cobalt M.: Trace Elements in Human and Animal Nutrition. In: W. MERTZ, eds. Academic Pres, San Diego. 143- 184, 1987.
Specker, B.L., Black, A., Allen, L., Morrow, F.: Low milk concentrations are related to low serum concentrations in vegetarian women and to methylmalonic aciduria in their infants. Am J Clin Nutr, 1990; 52: 1073- 1076.
State Planning Organization-SPO: The specialization comission report of food industry. Sub comission report of milk and milk products industry. 8th Development Plan. DPT-OiK, 2636/644, DPT,, Ankara, 2001.
State Institute of Statistics-SIS: Statistic yearbook of Turkey. http: //www.die.gov.tr, 2003.
Tan, S., Erturk, E.: Süt ve Süt Mamulleri Durum ve Tahmin Raporu, TEAE, Ankara, 2002.
Trugo, N.M., Sardinha, F.: Cobalamin and cobalamin-binding capacity in Human milk. Nutr Res, 1994; 14: 22- 33.

Details

Primary Language

Turkish

Subjects

-

Journal Section

-

Authors

Göknur Terzi This is me

Ufuk Kamber This is me

Cevat Nisbet This is me

Sena Çenesiz This is me

Publication Date

November 21, 2012

Submission Date

November 21, 2012

Acceptance Date

-

Published in Issue

Year 2008 Volume: 34 Number: 2

DOI

https://doi.org/10.16988/iuvfd.46753

IZ

https://izlik.org/JA83DK72CM

Cite

RIS / Bibtex

APA

Terzi, G., Kamber, U., Nisbet, C., & Çenesiz, S. (2012). DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 34(2), 7-13. https://doi.org/10.16988/iuvfd.46753

AMA

1.Terzi G, Kamber U, Nisbet C, Çenesiz S. DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE. iuvfd. 2012;34(2):7-13. doi:10.16988/iuvfd.46753

Chicago

Terzi, Göknur, Ufuk Kamber, Cevat Nisbet, and Sena Çenesiz. 2012. “DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 34 (2): 7-13. https://doi.org/10.16988/iuvfd.46753.

EndNote

Terzi G, Kamber U, Nisbet C, Çenesiz S (November 1, 2012) DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 34 2 7–13.

IEEE

[1]G. Terzi, U. Kamber, C. Nisbet, and S. Çenesiz, “DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE”, iuvfd, vol. 34, no. 2, pp. 7–13, Nov. 2012, doi: 10.16988/iuvfd.46753.

ISNAD

Terzi, Göknur - Kamber, Ufuk - Nisbet, Cevat - Çenesiz, Sena. “DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 34/2 (November 1, 2012): 7-13. https://doi.org/10.16988/iuvfd.46753.

JAMA

1.Terzi G, Kamber U, Nisbet C, Çenesiz S. DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE. iuvfd. 2012;34:7–13.

MLA

Terzi, Göknur, et al. “DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, vol. 34, no. 2, Nov. 2012, pp. 7-13, doi:10.16988/iuvfd.46753.

Vancouver

1.Göknur Terzi, Ufuk Kamber, Cevat Nisbet, Sena Çenesiz. DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE. iuvfd. 2012 Nov. 1;34(2):7-13. doi:10.16988/iuvfd.46753