DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE

Göknur Terzi; Ufuk Kamber; Cevat Nisbet; Sena Çenesiz

doi:10.16988/iuvfd.46753

DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE

Öz

ABSTRACT

Vitamin B₁₂ is a member of the vitamin B complex. It contains cobalt, and is also known as cobalamin. It is exclusively synthesised by bacteria and is found primarily in fish, meat, poultry, eggs, milk and milk products. Vitamin B₁₂deficiency have an underlying stomach or intestinal disorder that limits the absorption of vitamin B₁₂ and might be caused to megaloblastic anemia. In this study cow milk samples were collected from in two different region in Turkey. In milk samples the amount of vitamin B₁₂ was detected by using ELISA. The vitamin B₁₂ concentrations of milk samples obtained from the cows of Holstein, Jersey, Zavot and East Anatolian Red species were determined as 0.98µg/L, 1.15µg/L, 2.93µg/L and 1.56µg/L, respectively. The concentrations of vitamin B₁₂ in Zavot cows milk were higher than the other species.

Key words: Vitamin B₁₂, cow milk, ELISA.

FARKLI SIĞIR IRKLARINA AİT SÜT ÖRNEKLERİNDE B 12 VİTAMİN DÜZEYİNİN ELİSA TEKNİĞİ İLE TESPİTİ

ÖZET

B₁₂ vitamini, B kompleks vitaminlerin bir üyesidir. Bu vitamin kobalt içerdiğinden dolayı kobalamin olarak adlandırılmaktadır. B₁₂ vitamini balık, et, tavuk, yumurta ve süt ürünlerinde bulunur ve bakteriler tarafından sentezlenir. B₁₂ vitamini yetersizliğine bağlı olarak mide ve bağırsak bozukluklar, kol ve bacaklarda uyuşukluk ve megolablastik anemi şekillenir. Bu çalışmada farklı sığır ırklarına ait süt örneklerinde B₁₂ vitamin düzeyleri araştırıldı. Bu amaçla Samsun ve Kars bölgesinden Holştayn, Jersey, Zavot and Doğu Anadolu Kırmızısı sığır ırklarına ait süt örnekleri toplandı. Süt örneklerinde B₁₂ vitamini miktarı ELISA yöntemi ile tespit edildi. B₁₂ vitamini Holştayn, Jersey, Zavot and Doğu Anadolu Kırmızısı sığır ırklarına ait süt örneklerinde sırasıyla 0.98µg/L, 1.15µg/L, 2.93µg/L ve 1.56µg/L olarak bulundu. Yapılan istatistik çalışması sonucunda vitamin B₁₂ miktarı Zavot ırkına ait süt örneklerinde diğer ırklardan daha yüksek bulundu (p<0.05, ANOVA).

Anahtar Kelimeler: B₁₂ vitamini, inek sütü, ELISA.

Anahtar Kelimeler

B1 2 vitamini, inek sütü, ELISA

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

-

Yazarlar

Göknur Terzi Bu kişi benim

Ufuk Kamber Bu kişi benim

Cevat Nisbet Bu kişi benim

Sena Çenesiz Bu kişi benim

Yayımlanma Tarihi

21 Kasım 2012

Gönderilme Tarihi

21 Kasım 2012

Kabul Tarihi

-

Yayımlandığı Sayı

Yıl 2008 Cilt: 34 Sayı: 2

DOI

https://doi.org/10.16988/iuvfd.46753

IZ

https://izlik.org/JA83DK72CM

Kaynak Göster

RIS / Bibtex

APA

Terzi, G., Kamber, U., Nisbet, C., & Çenesiz, S. (2012). DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 34(2), 7-13. https://doi.org/10.16988/iuvfd.46753

AMA

1.Terzi G, Kamber U, Nisbet C, Çenesiz S. DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE. iuvfd. 2012;34(2):7-13. doi:10.16988/iuvfd.46753

Chicago

Terzi, Göknur, Ufuk Kamber, Cevat Nisbet, ve Sena Çenesiz. 2012. “DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 34 (2): 7-13. https://doi.org/10.16988/iuvfd.46753.

EndNote

Terzi G, Kamber U, Nisbet C, Çenesiz S (01 Kasım 2012) DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 34 2 7–13.

IEEE

[1]G. Terzi, U. Kamber, C. Nisbet, ve S. Çenesiz, “DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE”, iuvfd, c. 34, sy 2, ss. 7–13, Kas. 2012, doi: 10.16988/iuvfd.46753.

ISNAD

Terzi, Göknur - Kamber, Ufuk - Nisbet, Cevat - Çenesiz, Sena. “DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 34/2 (01 Kasım 2012): 7-13. https://doi.org/10.16988/iuvfd.46753.

JAMA

1.Terzi G, Kamber U, Nisbet C, Çenesiz S. DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE. iuvfd. 2012;34:7–13.

MLA

Terzi, Göknur, vd. “DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, c. 34, sy 2, Kasım 2012, ss. 7-13, doi:10.16988/iuvfd.46753.

Vancouver

1.Göknur Terzi, Ufuk Kamber, Cevat Nisbet, Sena Çenesiz. DETECTION OF VITAMIN B12 IN DIFFERENT COW MILK USING ELISA TECHNIQUE. iuvfd. 01 Kasım 2012;34(2):7-13. doi:10.16988/iuvfd.46753