THE PRESENCE OF STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCAL ENTEROTOXINS IN READY-TO-COOK MEATBALLS AND WHITE PICKLED CHEESE

Kamil Bostan; Ömer Çetin; Serkan Büyükünal; Özer Ergün

doi:10.16988/iuvfd.72724

THE PRESENCE OF STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCAL ENTEROTOXINS IN READY-TO-COOK MEATBALLS AND WHITE PICKLED CHEESE

Abstract

ABSTRACT

In this study 30 ready-to-cook meatball and 30 white pickled cheese samples, obtained from different sales outlets in Istanbul, were analysed for the presence of staphylococcal enterotoxins (SE) with the aid of the ELISA technique. Additionally, total Staphylococcus aureus count, DNase positive Staphylococcus aureus count and thermonuclease activity were determined. The meatball samples were found to contain between 3.0x10¹and 5.2x10⁴ cfu/g of Staphylococcus aureus while in the white cheese samples, the count varied between <10 - 9.2x10³ cfu/g. In 9 meatball samples and 11 white cheese samples, no DNase positive bacteria growth could be detected. Despite these results, SE could not be identified in any of the samples of both food groups, and all samples gave negative results in the thermonuclease test. With respect to Staphylococcus aureus count, 63% of the white cheese samples and 43% of the meatball samples did not comply with Turkish Food Codex. The findings of this study indicate a low microbiological quality of the analysed meatballs and white cheese samples. However, none of the samples conteined staphylococcal enterotoxins.

Keywords: Staphylococcus aureus, enterotoxin, cheese, meatball

HAZIR KÖFTE VE BEYAZ PEYNİRLERDE STAPHYLOCOCCUS AUREUS VE STAFİLOKOKKAL ENTEROTOKSİNLERİN VARLIĞI

ÖZET

Bu çalışmada Staphylococcus aureus yönünden riskli gıdalar arasında yer alan hazır köftelerde (30 adet) ve beyaz peynirlerde (30 adet) ELISA tekniği ile stafilokokkal enterotoksinlerin (SE) varlığı araştırılmıştır. Çalışma materyali İstanbul'daki çeşitli satış noktalarından sağlanmıştır. Örneklerde aynı zamanda toplam Staphylococcus aureus sayısı, DNase pozitif Staphylococcus aureus sayısı ve termonukleaz aktivitesi de belirlenmiştir. Yapılan analizler sonucunda hazır köfte örneklerinde 3.0X10¹ - 5.2x10⁴ kob/g; beyaz peynir örneklerinde <10 - 9.2x10³ kob/g arasinda Staphylococcus aureus saptanmıştır. Hazır köftelerin 9'unda, beyaz peynirlerin 11'inde DNase pozitif bakteri üremesi kaydedilmemiştir. Buna rağmen, her iki gıda grubuna ait örneklerin hiçbirinde SE saptanmamış ve tamamında termonukleaz testi negatif sonuç vermiştir. Staphylococcus aureus sayısı bakımından peynir örneklerinin % 63'u, hazır köfte örneklerinin %43'ü Türk Gıda Kodeksi Yönetmeliği'ne uygunluk göstermemektedir. Bu çalışmada elde edilen bulgular; incelenen köfte ve peynir örneklerinin mikrobiyolojik kalitesi düşük olmakla birlikte stafilokokkal enterotoksinlerin bulundurmadığını göstermektedir.

Anahtar Kelimeler: Staphylococcus aureus, enterotoksin, peynir, köfte

Keywords

Staphylococcus aureus, enterotoksin, peynir, köfte

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Details

Primary Language

Turkish

Subjects

-

Journal Section

-

Authors

Kamil Bostan

Ömer Çetin

Serkan Büyükünal This is me

Özer Ergün This is me

Publication Date

December 21, 2012

Submission Date

December 21, 2012

Acceptance Date

-

Published in Issue

Year 2006 Volume: 32 Number: 3

DOI

https://doi.org/10.16988/iuvfd.72724

IZ

https://izlik.org/JA45CB87PR

Cite

RIS / Bibtex

APA

Bostan, K., Çetin, Ö., Büyükünal, S., & Ergün, Ö. (2012). THE PRESENCE OF STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCAL ENTEROTOXINS IN READY-TO-COOK MEATBALLS AND WHITE PICKLED CHEESE. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 32(3), 31-39. https://doi.org/10.16988/iuvfd.72724

AMA

1.Bostan K, Çetin Ö, Büyükünal S, Ergün Ö. THE PRESENCE OF STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCAL ENTEROTOXINS IN READY-TO-COOK MEATBALLS AND WHITE PICKLED CHEESE. iuvfd. 2012;32(3):31-39. doi:10.16988/iuvfd.72724

Chicago

Bostan, Kamil, Ömer Çetin, Serkan Büyükünal, and Özer Ergün. 2012. “THE PRESENCE OF STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCAL ENTEROTOXINS IN READY-TO-COOK MEATBALLS AND WHITE PICKLED CHEESE”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 32 (3): 31-39. https://doi.org/10.16988/iuvfd.72724.

EndNote

Bostan K, Çetin Ö, Büyükünal S, Ergün Ö (December 1, 2012) THE PRESENCE OF STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCAL ENTEROTOXINS IN READY-TO-COOK MEATBALLS AND WHITE PICKLED CHEESE. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 32 3 31–39.

IEEE

[1]K. Bostan, Ö. Çetin, S. Büyükünal, and Ö. Ergün, “THE PRESENCE OF STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCAL ENTEROTOXINS IN READY-TO-COOK MEATBALLS AND WHITE PICKLED CHEESE”, iuvfd, vol. 32, no. 3, pp. 31–39, Dec. 2012, doi: 10.16988/iuvfd.72724.

ISNAD

Bostan, Kamil - Çetin, Ömer - Büyükünal, Serkan - Ergün, Özer. “THE PRESENCE OF STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCAL ENTEROTOXINS IN READY-TO-COOK MEATBALLS AND WHITE PICKLED CHEESE”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi 32/3 (December 1, 2012): 31-39. https://doi.org/10.16988/iuvfd.72724.

JAMA

1.Bostan K, Çetin Ö, Büyükünal S, Ergün Ö. THE PRESENCE OF STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCAL ENTEROTOXINS IN READY-TO-COOK MEATBALLS AND WHITE PICKLED CHEESE. iuvfd. 2012;32:31–39.

MLA

Bostan, Kamil, et al. “THE PRESENCE OF STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCAL ENTEROTOXINS IN READY-TO-COOK MEATBALLS AND WHITE PICKLED CHEESE”. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, vol. 32, no. 3, Dec. 2012, pp. 31-39, doi:10.16988/iuvfd.72724.

Vancouver

1.Kamil Bostan, Ömer Çetin, Serkan Büyükünal, Özer Ergün. THE PRESENCE OF STAPHYLOCOCCUS AUREUS AND STAPHYLOCOCCAL ENTEROTOXINS IN READY-TO-COOK MEATBALLS AND WHITE PICKLED CHEESE. iuvfd. 2012 Dec. 1;32(3):31-9. doi:10.16988/iuvfd.72724