Astımda Sitokin Düzeyi ve Oksidan yükün prognoza etkisi

Year 2023, Volume: 2 Issue: 4, 182 - 188, 25.12.2023

Nihal Akdeniz Ünal Fatma Evyapan

https://doi.org/10.57221/izmirtip.1284847

Abstract

GİRİŞ ve AMAÇ: Bu çalışmanın amacı astım ve atak sırasındaki sitokin düzeylerini ve inflamatuar parametreleri değerlendirmektir.
YÖNTEM ve GEREÇLER: 30 astım ataklı, 41 stabil astımlı ve 42 sağlıklı kontrol çalışmaya alındı. Tüm olgularda hemogram, C Reaktif protein(CRP), eritrosit sedimentasyon hızı(ESH), eritrositlerde indirgenmiş GSH, plazmada MDA ve ELISA yöntemi ile serum sitokin (IL-1β, IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, TNF-α) düzeyleri Enzyme-Linked ImmunoSorbent Assay (ELİSA) yöntemi ile çalışıldı.
BULGULAR: Gruplar arasında beyaz küre, nötrofil sayısı ortalamaları karşılaştırıldığında anlamlı fark saptandı (p<0,001, p=0,004). Atak sırasında ölçülen beyaz küre ve nötrofil sayısı ortalamasının, atak sonrasında azaldığı saptandı (p=0,045, p>0,05). Gruplar arasında ve atak sırasındaki ve sonrasındaki lenfosit sayısı ortalamaları karşılaştırıldığında anlamlı fark saptanmadı (p>0,05). Gruplar arasında ESH ve CRP ortalamaları karşılaştırıldığında anlamlı fark saptandı (p=0,001, p=0,005). Atak sırasındaki ESH ve CRP ortalamasının atak sonrasında azaldığı saptandı (p=0,610, p=0,306).Gruplar arasında ortalama MDA ve GSH düzeyleri karşılaştırıldığında anlamlı fark saptanmadı (p=0,107, p=0,386). Atak sırasındaki ortalama MDA düzeyi atak sonrasında azalırken, GSH ortalaması atak sonrasında artmış saptandı (p>0,05). Gruplar arasında ortalama IL-4, IL-6, IL-10, TNF-α düzeyleri açısından anlamlı fark saptanırken (p<0,001), ortalama IL-1β, IL-5, IL-8, IL-13 düzeyleri açısından anlamlı fark saptanmadı (p>0,05).
TARTIŞMA ve SONUÇ: Çalışma sonuçlarımıza göre, astım atak sırasındaki inflamasyon belirteçleri (beyazküre, nötrofil, CRP, ESH), oksidan yük göstergesi (MDA) ve sitokinler (IL-1β, IL-4, IL-5, IL-10) atak tedavisi sonrasında azalmıştır. Bu sonuçlar astım atağında oksidan yükün ve sitokinlerin arttığını teyit etmektedir.

Keywords

sitokinler, prognoz, astım

Supporting Institution

Pamukkale üniversitesi

Project Number

2007TPF010

Thanks

Pamukkale üniversitesi'ne desteklediği için teşekkür ederiz

Effect of Cytokine Level and Oxidant Load on Prognosis in Asthma

Abstract

INTRODUCTION and PURPOSE: The aim of this study is to evaluate cytokine levels and inflammatory parameters during asthma and attacks.
METHODS and MATERIALS: 30 asthmatic patients, 41 stable asthmatic patients and 42 healthy controls were included in the study. In all cases, hemogram, C Reactive protein (CRP), erythrocyte sedimentation rate (ESH), reduced GSH in erythrocytes, MDA in plasma and serum cytokines (IL-1β, IL-4, IL-5, IL-6, IL-8) by ELISA method. , IL-10, IL-13, TNF-α) levels were studied with the Enzyme-Linked ImmunoSorbent Assay (ELISA) method.
RESULTS: A significant difference was found when the white blood cell and neutrophil count averages were compared between the groups (p<0.001, p=0.004). It was determined that the average white blood cell and neutrophil count measured during the attack decreased after the attack (p=0.045, p>0.05). When the average lymphocyte count was compared between the groups and during and after the attack, no significant difference was detected (p>0.05). When the ESR and CRP averages were compared between the groups, a significant difference was detected (p = 0.001, p = 0.005). It was determined that the mean ESH and CRP levels during the attack decreased after the attack (p=0.610, p=0.306). When the mean MDA and GSH levels were compared between the groups, no significant difference was detected (p=0.107, p=0.386). While the average MDA level during the attack decreased after the attack, the average GSH level was found to increase after the attack (p>0.05). While there was a significant difference between the groups in terms of mean IL-4, IL-6, IL-10, TNF-α levels (p<0.001), no significant difference was found in mean IL-1β, IL-5, IL-8, IL-13 levels. (p>0.05).
DISCUSSION AND CONCLUSION: According to our study results, inflammation markers (white blood cell, neutrophil, CRP, ESH), oxidant load indicator (MDA) and cytokines (IL-1β, IL-4, IL-5, IL-10) during an asthma attack were affected by the attack treatment. decreased afterwards. These results confirm that oxidant load and cytokines increase during an asthma attack.

Keywords

cytokines, prognosis, asthma

Project Number

2007TPF010

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