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Isolation and Identification of Bacteria Causes Diseases on the Tomato (Solanum Lycopersicum L.) in Igdır

Year 2021, , 108 - 129, 31.12.2021
https://doi.org/10.46876/ja.1015781

Abstract

In this study, bacterial disease agents that cause yield and quality loss in tomatoes in Iğdır province in 2017 were investigated. For this purpose, diseased plant samples were collected by conducting surveys in the Aralık, Karakoyun, Tuzluca, Kasımcan, Oba and Melekli, where production is intense. 98 bacterial strains were obtained in the isolation study. Fluorescent pigment production in King B medium, levan colony formation, gram reaction, pectolytic activity, oxidase, amylase, arginine dehydrolase, catalase and hypersensitivity reaction properties in tobacco plant were tested in King B medium of strains. Pathogenicity test was found positive for 11 strains on super 5656 tomato cultivar. Strain were identified by using fatty acid methil ester analysis (FAME) and Biolog Gen III system. Acording to identfication results 11 strain were determined, of which 8 were Xanthomonas axonopodis pv. vesicatoria, 2 were Pseudomonas viridiflava and 1 was Pseudomonas syringae pv. tomato. The presence of pathogens in Iğdır province was proved for the first time by this study.

Project Number

2017-FBE-A21

References

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  • Bathily, H., Babana, A.H., Samake, F., 2010. Bacillus Pumilus, A New Pathogen on Potato Tubers in Storage in Mali. African Journal of Microbiology Research, 4(20), 2067-2071.
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  • Black, R., Seal, S., Abubakar, Z., Nono-Womdim, R., Swai, I., 2001. Bacterial Spot (Xanthomonas campestris pv. vesicatoria) of Tomato and Sweet Pepper in Tanzania. Plant Pathology, 50,810.
  • Bouzar, H., Jones, J.B., Stall, R.E., Hodge, N.C., Minsavage, G.V., Benedict, A.A. Alvarez, A.M., 1994. Physiological, Chemical, Serological Nad Pathogenic Analyses of A Worldwide Collection of Xanthomonas campestris pv. vesicatoria. Phytopathology, 84,663-671.
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Iğdır’da Domates (Solanum Lycopersicon L.)’te Hastalığa Neden Olan Bakterilerin İzolasyonu ve Tanısı

Year 2021, , 108 - 129, 31.12.2021
https://doi.org/10.46876/ja.1015781

Abstract

Bu çalışmada, 2017 yılında Iğdır ilinde domateste verim ve kalite kaybına neden olan bakteriyel hastalık etmenleri araştırılmıştır. Bu amaçla üretiminin yoğun olarak yapıldığı Aralık, Karakoyun, Tuzluca, Kasımcan, Oba ve Melekli ilçelerinde sürvey yapılarak hastalıklı bitki örnekleri toplanmıştır. İzolasyon çalışması sonucunda 98 bakteri straini elde edilmiştir. Strainlerin King B besiyerinde fluoresant pigment üretimi, levan koloni oluşumu, gram reaksiyon, pektolitik aktivite, oksidaz, amilaz, arginine dehidrolaz, katalaz ve tütün bitkisinde hipersensetif reaksiyon özellikleri test edilmiştir. Süper 5656 domates çeşitinde 11 strainin patojenisite testi pozitif bulunmuştur. Yağ asit metil ester analizi (FAME) ve BIOLOG Gen III ile 8 strain Xanthomonas axonopodis pv. vesicatoria, 2 strain Pseudomonas viridiflava ve 1 strain Pseudomonas syringae pv. tomato olarak tanılanmıştır. Iğdır ilinde belirtilen patojenlerin varlığı ilk defa bu çalışma ile ortaya konulmuştur.

Supporting Institution

Iğdır Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

2017-FBE-A21

Thanks

2017-FBE-A21 numaralı projenin desteklenmesinde verdikleri katkıdan dolayı Iğdır Üniversitesi Bilimsel Araştırma Projeleri Birimine teşekkür ederiz.

References

  • Aksoy, H. M., 2002.Samsun ilinde Domates Bakteriyel Hastalkları ve Yaygınlıkları. Ankara Üniversitesi/ Fen Bilimleri Enstitüsü/Bitki Koruma Ana Bilim Dalı.. Doktora Tezi, 118s.
  • Aysan, Y., Yıldız, N., Yucel, F., 2004. Identification of Pseudomonas viridiflava on Tomato by Traditional Methods and Enzyme-Linked Immunosrbent Assay. Phytopathology/Mycology, 32 (2), 146-153.
  • Aysan, Y., Mırık. M., Çetınkaya, Yıldız, R., Küsek, M., 2005. Pseudomonas syringae pv. tomato’nun Yayılmasında Tohum Kökenli İnokulumun Rolü. Türkiye II. Tohumculuk Kongresi. 353 (9-11) ,Adana.
  • Basım, H., 2004. Bacterial Spot of Tomato and Pepper Caused by Xanthomonas axonopodis pv. vesicatoria in the Western Mediterranean Region of Turkey. Plant Disease, (1), 85.
  • Bathily, H., Babana, A.H., Samake, F., 2010. Bacillus Pumilus, A New Pathogen on Potato Tubers in Storage in Mali. African Journal of Microbiology Research, 4(20), 2067-2071.
  • Bergey, D.H., Harrison, F.C., Breed, R.S., Hammer, B.W., Huntoon, F. M., 1923. Bergey’s Manual of Determinative Bacteriology, The Williams & Wilkins Co., Baltimore, 116 P.
  • Black, R., Seal, S., Abubakar, Z., Nono-Womdim, R., Swai, I., 2001. Bacterial Spot (Xanthomonas campestris pv. vesicatoria) of Tomato and Sweet Pepper in Tanzania. Plant Pathology, 50,810.
  • Bouzar, H., Jones, J.B., Stall, R.E., Hodge, N.C., Minsavage, G.V., Benedict, A.A. Alvarez, A.M., 1994. Physiological, Chemical, Serological Nad Pathogenic Analyses of A Worldwide Collection of Xanthomonas campestris pv. vesicatoria. Phytopathology, 84,663-671.
  • Cruz, L., Cruz, J., Eloy, M., Oliveira, H., Vaz, H., Tenreiro, R., 2010. First Report of Bacterial Speck of Tomato Caused by Pseudomonas syringae pv. tomato Race 1 in Portugal. Journal Article. Plant Disease. 94(12), 1504-1504
  • Çetinkaya Yıldız R. ve Aysan Y., 2008. Domates Bakteriyel solgunluk Hastalığı etmeni (C. michiganensis subsp. Michiganensi)’nin İzolasyonu, G eleneksel, Serolojik ve Moleküler Yöntemlerle Tanısı. Çukurova Üniversitesi Fen Bilimleri Enstitüsü. 173s.
  • Dadaşoğlu, F., 2013. Artvin Erzincan Erzurum Iğdır İllerinde Bazı Meyve ve Sebzelerde Yumuşak Çürüklüğe Sebep Olan Bakterilerin İzolasyonu Klasik ve Moleküler Yöntemler ile Karakterizasyonu. Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Doktora Tezi, s143.
  • Davis, M.J., Gillaspie, A. G., Jr. Vidaver, A. K., Harris, R. W., 1984. Clavibacter: A New Genus Containing Some Phytopathogenic Coryneform Bacteria, İncluding Clavibacterxyli subsp. xyli sp. Nov., subsp. Nov. and Clavibacter xyli subsp. cynodontis subsp. Nov., Pathogens That Cause Ratoon Stunting Disease of Sugarcane and Bermudagrass Stunting Disease, International Journal of Systemic Bacteriology, 34, 107–17.
  • Dönmez, M. F., 2004. Erzurum ve Erzincan İllerinde Fasulye (Phaseolus Vulgaris L.) Bitkisinde Görülen Bakteriyel Hastalık Etmenlerinin Tanılanması ve Pseudomonas syringae pv. phaseolicola ve Xanthomonas campestris pv. phaseolf 'ye Karşı Çeşitli Fasulye Genotip ve Çeşitlerinin Duyarlılıklarının Belirlenmesi. Atatürk Üniversitesi / Fen Bilimleri Enstitüsü / Bitki Koruma Anabilim Dalı. s305..
  • FAO, 2020. Food and Agriculture Organization of The United Nations. Tomatoes Growing in The World. http://www.fao.org/faostat/en/#data/QC (Erişim Tarihi: 24.02.2020).
  • Gardner, M.W., Kendrick, J.B., 1923. Bacterial Spot of Tomato and Pepper, Phytopathology 13:307-315.
  • Gasic, K., Prokic, A., Ivanovic, M., Kuzmanovic, N., Obradovic, A., 2012. Differentation of Pseudomonas syringae Pathovars Originating From Stone Fruits. Pesticides. Phytomedicine, 27(3),201-229.
  • Gonzalez, C.F., Ackerley, D.F., Park, C.H., Matın, A., 2003. A Soluble Flavoprotein Contributes To Chromate Reduction and Tolerance by Pseudomonas putida. Acta Biotechnol. 2(3), 233-239.
  • Goumans, D.E., Chatzaki, A.K., 1998. Characterization and Host Range Evaluation of Pseudomonas viridiflava From Melon, Blite, Tomato, Chrysanthemum and Eggplant. European Journal of Plant Pathology, 104,181-188.
  • Gök, G., 2016. Iğdır İli Elma Ağaçlarında Ateş Yanıklığı Hastalığına Neden Olan Erwinia amylovora (Burr.) Wınslow et al. Etmeninin Biyokimyasal ve Moleküler (MIS) Yöntemle Tanısı. Iğdır Üniversitesi, Fen Bilimleri Enstitüsü Yüksek Lisans Tezi, s72.
  • Ibrahim, Y., Al-Saleh, M., 2012. First Report of Bacterial Spot Caused by Xanthomonas campestris pv. vesicatoria on Sweet Pepper (Capsicum annuum L.) in Saudi Arabia. Plant Disease, 96(11), 1690-1690.
  • İmriz G.ve Çınar Ö., 2015. Domates Öz Nekrozu Etmenlerinden Pseudomonas cichorii ve Pseudomonas corrugata’nın ELİSA’ya Dayalı ve Geleneksel Tanıtemleri ile Teşhisi. Vol. 3, no 7-13
  • Karagöz, K., 2013. Erzurum İli Patates Tarlalarından İzole Edilen Bitki Patojeni Streptomyces Türlerinin Tanısı ve Karakterizosyonu. Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Doktora Tezi, s129.
  • Klement, Z., Farkas, G. L., Lourekovich, L., 1966. Hypersensitive Reaction Induced by Phytopathogenic Bacteria in Tobacco Leaf, Phytopathology, 54, 474-477.
  • Klement, Z., Rudolph, K., Sands, D.C., 1990. Methods in Phytobacteriology, Akademia Kiado, Budapest, XIV+568s. Lamichhane J.R., Balestra G.M., Varvaro L., 2010. First report of bacterial spot caused by Xanthomonas campestris pv. vesicatoria race 2 on tomato in Nepal. New Diseases Reports, 22, p25.
  • Lelliott, R.A., Stead, D.E., 1987. Methods For The Diagnosis of Bacterial Diseases of Plants. Blacwell Scientific Publications. p216.
  • Lue Y. S., Deng W. L., Wu Y. F., Cheng A. S., Hsu S. T.. and Tzeng K. C., 2010. Characterization of Xanthomonas Associated with Bacterial Spot of Tomato and Pepper in Taiwan. Plant Pathology Bulletin 19: 181-190.
  • Mensi, I., Jabnoun-Khiareddine, H., Zarrougui, N.E., Zahra, H., Cesbron, S., Jacques, M. A., Daami-Remadi, M., 2018. First Report of Tomato Bacterial Speck Caused By Pseudomonas syringae pv. tomato in Tunisia. New Disease Reports, 38, 21.
  • Mılıjasevic, S., Todorovic, B., Rekanovic, E., Potocnik I., Gavrilovic, V., 2009. Races and Hosts of Pseudomonas syringae pv. tomato in Serbia. Archives of Biological Sciences, 61 (1), 93-102.
  • Narayanasamy, P., 1997. Plant Pathogen Detection and Disease Diagnosis, pp. 331.
  • Roy, A., 1988. Use of Fatty Acid For The Identification of Phytopathogenic Bacteria. Plant Disease, 72, 460.
  • Popović, T., Ivanović, Z., 2015. First Report of Pseudomonas viridiflava Causing Pith Necrosis of Tomato (Solanum Lycopersicum) in Serbia. Diseases Notes, 99 (7), 1033.
  • Sarris, F.P., Trantas, E. A., Mpalantinaki, E., Ververidis, F., Goumas, D.E., 2012. Pseudomonas viridiflava, A Multı Host Plant Pathogen With Significant Genetic Variation At The Molecular Level. Plasone, 7, Issue 4,
  • Sasser, M., 1990. İdendification of bacteria by gas chomatography of celluler fatty acids. MIDI, Technical Note. 101, 1-6
  • Saygılı, H., 1995. Fitobakteriyoloji. Doğruluk Matbaası, İzmir, pp. 203.
  • Scarlett, C.A., Fletcher, J.T., Roberts P., Lelliott, R.A., 1978, Tomato Pith Necrosis Caused by Pseudomonas corrugata, Annals of Applied Biology, 88,105-114.
  • Schaad, N. W., 1994. Laboratory Guide for Identification of Plant Pathogenic Bacteria. APS Press, pp. 164.
  • Schaad, N,W., Jones, J.B., Chun, W., 2001. Laboratory Guide For Identification of Plant Pathogenic Bacteria. The American Phytopathological Society, St. Paul, MN., USA.
  • Shenge, K.C., Mabagala, R.B., 2007. First Report of Bacterial Speck of Tomato Caused by Pseudomonas syringae pv. tomato in Tanzania. Plant Diseas. (4), 462.
  • Sijam, K., Chang, C.J., Gitaitis, R.D., 1992. A Medium for Differentiating Tomato and Pepper Strains of Xanthomonas campestris pv. vesicatoria Can. Journal Plant Pathol. 14,162-164.
  • Stancu, M. and Rodi, M., 2020. Identification and Biochemical Characterization of some Strains of Clavibacter michiganensis subsp. Michiganensis. University of Craiova, Faculty of Horticulture, A.I. Cuza, Craiova. Romania. no. 13.
  • Şahın, F., Kotan, R., 1999. First Observation of Xanthomonas campestris pv. vesicatoria Race T2P7 Isolated From Pepper in The Philippines. Plant Disease, 83(6), 590.
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There are 55 citations in total.

Details

Primary Language Turkish
Subjects Agricultural Engineering
Journal Section Research Articles
Authors

Büşran Sunyar This is me 0000-0001-8524-3308

Mesude Figen Dönmez 0000-0002-7992-8252

İrfan Çoruh 0000-0002-6569-6163

Project Number 2017-FBE-A21
Publication Date December 31, 2021
Submission Date October 28, 2021
Acceptance Date December 26, 2021
Published in Issue Year 2021

Cite

APA Sunyar, B., Dönmez, M. F., & Çoruh, İ. (2021). Iğdır’da Domates (Solanum Lycopersicon L.)’te Hastalığa Neden Olan Bakterilerin İzolasyonu ve Tanısı. Journal of Agriculture, 4(2), 108-129. https://doi.org/10.46876/ja.1015781