Synthesis, Characterization of Biogenic Copper Nanoparticles and Their Therapeutic Activity

Year 2023, , 26 - 35, 30.06.2023

Yılmaz Koçak , Hamdullah Seçkin

https://doi.org/10.46876/ja.1287833

Cited By: 1

Abstract

Green synthesis of copper nanoparticles (Cu NPs) is an economical, environmentally friendly and non-toxic approach that has been the subject of research in health and industry. Therefore, in this study, Cu NPs were synthesized using Pimpinella anisum (P.anisum) seed extract and their pharmacological activities were evaluated. Characterization of Cu NPs was performed by UV-vis, FT-IR and SEM-EDX analyses. Copper metal was reduced by reacting with the seed extract and reached the maximum peak at 385 nm in the UV-vis spectra, confirming the surface plasmon resonance. FT-IR spectroscopy showed the participation of phytochemical components in P. anisum in the synthesis. SEM analysis determined that the size of the biosynthesized nanoparticles is 10-20 nm in diameter and has a spherical structure. Strong signals of copper metal were confirmed by EDX analysis. The therapeutic effect of Cu NPs was evaluated by antioxidant and antibacterial assays. The DPPH radical scavenging activity IC50 inhibition values of Cu NPs were better than the seed extract and exhibited strong antioxidant activity. Antibacterial activity was performed by the disk diffusion method and Cu NPs were more effective against gram-positive bacteria. It had the highest zone diameter (18.0±2.8 mm), especially on Bacillus subtilis bacteria. These results showed that Cu NPs may have a selective effect against drug-resistant bacteria as an alternative agent to pharmaceutical applications. This study showed that P. anisum seed extract-mediated bioconjugation of Cu NPs can be done simply, quickly and cost-effectively. As a result, Cu NPs should be supported by more detailed in vivo studies to create antioxidant and antibacterial agents.

Keywords

Antibacterial, Antioxidant, Copper nanoparticles, Pimpinella anisum, Green synthesis

Supporting Institution

YOK

Project Number

YOK

Thanks

The authors would like to thank the Science Application and Research Center, Van Yuzuncu Yil University.

References

• Abbasi, A., Khojasteh, H., Hamadanian, M., & Salavati-Niasari, M. (2016). Synthesis of CoFe2O4 nanoparticles and investigation of the temperature, surfactant, capping agent and time effects on the size and magnetic properties. Journal of Materials Science: Materials in Electronics, 27(5), 4972–4980. https://doi.org/10.1007/S10854-016-4383-Y
• Adewale Akintelu, S., Kolawole Oyebamiji, A., Charles Olugbeko, S., & Felix Latona, D. (2021). Green chemistry approach towards the synthesis of copper nanoparticles and its potential applications as therapeutic agents and environmental control. Current Research in Green and Sustainable Chemistry, 4(September), 100176. https://doi.org/10.1016/j.crgsc.2021.100176
• Alkhulaifi, M. M., Alshehri, J. H., Alwehaibi, M. A., Awad, M. A., Al-Enazi, N. M., Aldosari, N. S., Hatamleh, A. A., & Abdel-Raouf, N. (2020). Green synthesis of silver nanoparticles using Citrus limon peels and evaluation of their antibacterial and cytotoxic properties. Saudi Journal of Biological Sciences, 27(12), 3434–3441. https://doi.org/10.1016/J.SJBS.2020.09.031
• Amaliyah, S., Pangesti, D. P., Masruri, M., Sabarudin, A., & Sumitro, S. B. (2020). Green synthesis and characterization of copper nanoparticles using Piper retrofractum Vahl extract as bioreductor and capping agent. Heliyon, 6(8), e04636. https://doi.org/10.1016/j.heliyon.2020.e04636
• Asghar, M. A., & Asghar, M. A. (2020). Green synthesized and characterized copper nanoparticles using various new plants extracts aggravate microbial cell membrane damage after interaction with lipopolysaccharide. International Journal of Biological Macromolecules, 160, 1168–1176. https://doi.org/10.1016/J.IJBIOMAC.2020.05.198
• Bazancir, N., & Meydan, I. (2022). Characterization of Zn nanoparticles of Platonus orientalis plant, investigation of DPPH radical extinquishing and antimicrobial activity. Eastern Journal of Medicine, 27(4). https://doi.org/10.5505/ejm.2022.34392
• Cho, K. H., Park, J. E., Osaka, T., & Park, S. G. (2005). The study of antimicrobial activity and preservative effects of nanosilver ingredient. Electrochimica Acta, 51(5), 956–960. https://doi.org/10.1016/J.ELECTACTA.2005.04.071
• Das, P. E., Abu‐Yousef, I. A., Majdalawieh, A. F., Narasimhan, S., & Poltronieri, P. (2020). Green Synthesis of Encapsulated Copper Nanoparticles Using a Hydroalcoholic Extract of Moringa oleifera Leaves and Assessment of Their Antioxidant and Antimicrobial Activities. Molecules, 25(3), 555. https://doi.org/10.3390/molecules25030555
• Ginting, B., Maulana, I., & Karnila, I. (2020). Biosynthesis Copper Nanoparticles using Blumea balsamifera Leaf Extracts: Characterization of its Antioxidant and Cytotoxicity Activities. Surfaces and Interfaces, 21, 100799. https://doi.org/10.1016/J.SURFIN.2020.100799
• Gopalakrishnan, V. & Muniraj, S. (2021). Neem flower extract assisted green synthesis of copper nanoparticles – Optimisation, characterisation and anti-bacterial study. Materials Today: Proceedings, 36, 832–836. https://doi.org/10.1016/J.MATPR.2020.07.013
• Gunalan, S., Sivaraj, R., & Venckatesh, R. (2012). Aloe barbadensis Miller mediated green synthesis of mono-disperse copper oxide nanoparticles: Optical properties. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 97, 1140–1144. https://doi.org/10.1016/J.SAA.2012.07.096 Is saabadi, Z., Nasrollahzadeh, M., & Sajadi, S. M. (2017). Green synthesis of the copper nanoparticles supported on bentonite and investigation of its catalytic activity. Journal of Cleaner Production, 142, 3584–3591. https://doi.org/10.1016/J.JCLEPRO.2016.10.109
• Keerthika, E., Ishwarya, K., Jayashree, L., Maripandian, S., Nivetha, C., & Sai, I. (2021). Potential Antibacterial Activity of Green Synthesized Copper Nanoparticles and its Characterization. International Journal of Current Research and Review, 13(19), 27–32. https://doi.org/10.31782/ijcrr.2021.131929
• Keihan, A. H., Veisi, H., & Veasi, H. (2017). Green synthesis and characterization of spherical copper nanoparticles as organometallic antibacterial agent. Applied Organometallic Chemistry, 31(7), e3642. https://doi.org/10.1002/AOC.3642
• Kocak, Y., Meydan, I., Gur Karahan, T., & Sen, F. (2023). Investigation of mycosynthesized silver nanoparticles by the mushroom Pleurotus eryngii in biomedical applications. International Journal of Environmental Science and Technology, 1–12. https://doi.org/10.1007/S13762-023-04786-Z/FIGURES/8
• Kocak, Y., Oto, G., Meydan, I., Seckin, H., Gur, T., Aygun, A., & Sen, F. (2022). Assessment of therapeutic potential of silver nanoparticles synthesized by Ferula Pseudalliacea rech. F. plant. Inorganic Chemistry Communications, 140, 109423. https://doi.org/10.1016/J.INOCHE.2022.109417
• Liu, H., Wang, G., Liu, J., Nan, K., Zhang, J., Guo, L., & Liu, Y. (2021). Green synthesis of copper nanoparticles using Cinnamomum zelanicum extract and its applications as a highly efficient antioxidant and anti-human lung carcinoma. Journal of Experimental Nanoscience, 16(1), 411–423. https://doi.org/10.1080/17458080.2021.1991577
• Mehdizadeh, T., Zamani, A., & Abtahi Froushani, S. M. (2020). Preparation of Cu nanoparticles fixed on cellulosic walnut shell material and investigation of its antibacterial, antioxidant and anticancer effects. Heliyon, 6(3), e03528. https://doi.org/10.1016/J.HELIYON.2020.E03528
• Meydan, I., & Seckin, H. (2021). Green synthesis, characterization, antimicrobial and antioxidant activities of zinc oxide nanoparticles using Helichrysum arenarium extract. International Journal of Agriculture Environment and Food Sciences, 5(1), 33-41. https://doi.org/10.31015/jaefs.2021.1.5
• Morrison, L., & Zembower, T. R. (2020). Antimicrobial Resistance. Gastrointestinal Endoscopy Clinics of North America, 30(4), 619–635. https://doi.org/10.1016/j.giec.2020.06.004
• Pyo, Y. H., Lee, T. C., Logendra, L., & Rosen, R. T. (2004). Antioxidant activity and phenolic compounds of Swiss chard (Beta vulgaris subspecies cycla) extracts. Food Chemistry, 85(1), 19–26. https://doi.org/10.1016/S0308-8146(03)00294-2
• Rabiee, N., Bagherzadeh, M., Kiani, M., Ghadiri, A. M., Etessamifar, F., Jaberizadeh, A. H., & Shakeri, A. (2020). Biosynthesis of copper oxide nanoparticles with potential biomedical applications. International Journal of Nanomedicine, 15, 3983–3999. https://doi.org/10.2147/IJN.S255398
• Rajesh, K. M., Ajitha, B., Reddy, Y. A. K., Suneetha, Y., & Reddy, P. S. (2018). Assisted green synthesis of copper nanoparticles using Syzygium aromaticum bud extract: Physical, optical and antimicrobial properties. Optik, 154, 593–600. https://doi.org/10.1016/j.ijleo.2017.10.074
• Rajeshkumar, S., Menon, S., Venkat Kumar, S., Tambuwala, M. M., Bakshi, H. A., Mehta, M., Satija, S., Gupta, G., Chellappan, D. K., Thangavelu, L., & Dua, K. (2019). Antibacterial and antioxidant potential of biosynthesized copper nanoparticles mediated through Cissus arnotiana plant extract. Journal of Photochemistry and Photobiology B: Biology, 197, 111531. https://doi.org/10.1016/J.JPHOTOBIOL.2019.111531
• Rajeshkumar, S., & Rinitha, G. (2018). Nanostructural characterization of antimicrobial and antioxidant copper nanoparticles synthesized using novel Persea americana seeds. OpenNano, 3, 18–27. https://doi.org/10.1016/J.ONANO.2018.03.001
• Rastogi, L., & Arunachalam, J. (2011). Sunlight based irradiation strategy for rapid green synthesis of highly stable silver nanoparticles using aqueous garlic (Allium sativum) extract and their antibacterial potential. Materials Chemistry and Physics, 129(1–2), 558–563. https://doi.org/10.1016/J.MATCHEMPHYS.2011.04.068
• Saif, S., Tahir, A., Asim, T., & Chen, Y. (2016). Plant mediated green synthesis of CuO nanoparticles: Comparison of toxicity of engineered and plant mediated CuO nanoparticles towards Daphnia magna. Nanomaterials, 6(11), 1–15. https://doi.org/10.3390/nano6110205
• Seçkin, H. (2021). Antimicrobial, Antioxidant and DNA Damage Prevention Effect of Nano-Copper Particles Obtained from Diplotaenia turcica Plant by Green Synthesis. Polish Journal of Environmental Studies, 30(5), 4187-4194. https://doi.org/10.15244/pjoes/132313 Senthilkumar, S. R., & Sivakumar, T. (2014). Green tea (Camellia sinensis) mediated synthesis of zinc oxide (ZnO) nanoparticles and studies on their antimicrobial activities. International Journal of Pharmacy and Pharmaceutical Sciences, 6(6), 461–465.
• Sihoglu Tepe, A., & Tepe, B. (2015). Traditional use, biological activity potential and toxicity of Pimpinella species. Industrial Crops and Products, 69, 153–166. https://doi.org/10.1016/J.INDCROP.2015.01.069
• Sinha, T., Adhikari, P. P., & Bhandari, V. M. (2022). Sustainable Fabrication of Copper Nanoparticles: A Potent and Affordable Candidate for Water Treatment, Water Disinfection, Antioxidant Activity and Theranostic Agent. ChemistrySelect, 7(15), e202103552. https://doi.org/10.1002/SLCT.202103552
• Subbaiya, R., & Masilamani Selvam, M. (2015). Green synthesis of copper nanoparticles from Hibicus rosasinensis and their antimicrobial, antioxidant activities. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 6(2), 1183–1190.
• Thanh, N. T. K., & Green, L. A. W. (2010). Functionalisation of nanoparticles for biomedical applications. Nano Today, 5(3), 213–230. https://doi.org/10.1016/J.NANTOD.2010.05.003
• Thiruvengadam, M., Chung, I. M., Gomathi, T., Ansari, M. A., Gopiesh Khanna, V., Babu, V., & Rajakumar, G. (2019). Synthesis, characterization and pharmacological potential of green synthesized copper nanoparticles. Bioprocess and Biosystems Engineering, 42(11), 1769–1777. https://doi.org/10.1007/S00449-019-02173-Y/FIGURES/5
• Wu, S., Rajeshkumar, S., Madasamy, M., & Mahendran, V. (2020). Green synthesis of copper nanoparticles using Cissus vitiginea and its antioxidant and antibacterial activity against urinary tract infection pathogens. Artificial Cells, Nanomedicine and Biotechnology, 48(1), 1153–1158. https://doi.org/10.1080/21691401.2020.1817053
• Xiong, J., Wang, Y., Xue, Q., & Wu, X. (2011). Synthesis of highly stable dispersions of nanosized copper particles using L-ascorbic acid. Green Chemistry, 13(4), 900–904. https://doi.org/10.1039/C0GC00772B
• Xu, D., Li, E., Karmakar, B., Awwad, N. S., Ibrahium, H. A., Osman, H. E. H., El-kott, A. F., & Abdel-Daim, M. M. (2022). Green preparation of copper nanoparticle-loaded chitosan/alginate bio-composite: Investigation of its cytotoxicity, antioxidant and anti-human breast cancer properties. Arabian Journal of Chemistry, 15(3), 103638. https://doi.org/10.1016/J.ARABJC.2021.103638
• Zayed, M. F., Mahfoze, R. A., El-kousy, S. M., & Al-Ashkar, E. A. (2020). In-vitro antioxidant and antimicrobial activities of metal nanoparticles biosynthesized using optimized Pimpinella anisum extract. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 585(November 2019), 124167. https://doi.org/10.1016/j.colsurfa.2019.124167