Weed Contamination in Seed Production: Dissemination Pathways and Integrated Prevention Strategies

Abstract

Weeds reduce crop yield and quality and threaten seed purity by spreading through natural (gravity, wind, water, vegetative/ballistic), animal-mediated (ecto- and endozoosaur-induced), and human-mediated (agricultural machinery, seedlings/saplings and other vegetative materials, infested crop seeds, trade, and transportation). Infestation, which occurs in seed production systems due to inadequate post-harvest cleaning of seed, is the most critical transmission route, as infested seed leads to the transport of weed propagules to clean fields. This review outlines the primary weed spread mechanisms, identifies critical stages in the seed production chain where contamination intensifies, and evaluates preventative management strategies. Preventing contamination is forms the foundation of effective weed management.The most crucial step in this approach is the consistent use of certified, clean seed.Certified seed is material that has undergone field and laboratory testing for genetic, physical, and biological quality. Complementary to this practice, cultural practices such as proper harvest timing, ensuring combine residue and equipment hygiene, reducing spread from feed and fertilizer (clean feed, adequate fermentation), and crop rotation, use of competitive varieties, maintenance of irrigation/drainage systems, and early interventions before seed set are critical. Overall, a prevention-based, integrated strategy to limit weed infestation in seed production not only ensures the preservation of seed quality but also stands out as a strategic imperative that supports long-term agricultural sustainability and food security.

Keywords

• Weed seed contamination
• Certified seed
• Spread pathways
• Equipment hygiene
• Crop rotation
• İntegrated weed management

References

1. Akhter, M. J., Sønderskov, M., Loddo, D., Ulber, L., Hull, R., Kudsk, P., 2023. Opportunities and challenges for harvest weed seed control in European cropping systems. European Journal of Agronomy, 142, 126639. https://doi.org/10.1016/j.eja.2022.126639.
2. Alexandratos, N., Bruinsma, J., 2012. World agriculture towards 2030/2050: the 2012 revision. Food and Agriculture Organization of the United Nations 12. https://doi.org/10.22004/ag.econ.288998.
3. Alptekin, H., Gürbüz, R., 2022. The effect of organic mulch materials on weed control in cucumber (Cucumis sativus L.) Cultivation. Journal of Agriculture, 5(1),68-79. https://doi.org/10.46876/ja.1126331.
4. Ansong, M., 2016. Mechanisms of seed dispersal and implications for weed management. Journal of Plant Ecology, 9(2), 110–121.
5. Ballard, L. A. T., 1958. Studies of dormancy in the seeds of subterranean clover (Trifolium subterraneum L.) I. Breaking of dormancy by carbon dioxide and by activated carbon. Australian Journal of Biological Sciences, 11(3), 246-260.
6. Baltzinger, C., Karimi, S.,Shukla, U., 2019. Plants on the move:Hitch-hiking with ungulates distributes diaspores across landscapes.Frontiers in Ecology and Evolution,7,38.https://doi.org/10.3389/fevo.2019.00038.
7. Benvenuti, S., 2007. Weed seed movement and dispersal strategies in the agricultural environment, Weed Biol. Manag. 7, 141–157.https://doi.org/10.1111/j.1445-6664.2007.00249.x.
8. Bozkurt, M. M., Tursun, N., 2018. Muş ilinde buğday ürününe karışan yabancı ot tohumları. Turkish Journal of Weed Science, 21(2), 1–15.

9. Chauhan, B,S., Johnson, D. E., 2010. The role of seed ecology in improving weed management strategies in the tropics. Advances in Agronomy,105: 221-262. doi:10.1016/S0065-2113(10)05006-6.
10. Couvreur, M., Cosyns, E., Hermy, M., Hoffmann, M., 2005., Complementarity of epi- and endozoochory of plant seeds by free ranging donkeys. Ecography, 28: 37-48. https://doi.org/10.1111/j.0906-7590.2005.04159.x.
11. FAO., 2019. The state of the world’s biodiversity for food and agriculture. Rome, Italy: Food and Agriculture Organization of the United Nations.https://openknowledge.fao.org/handle/20.500.14283/ca3229en. (Accessed: 15 Sept 2025).
12. FAO., 2020. The state of food security and nutrition in the world 2020. Rome, Italy: Food and Agriculture Organization of the United Nations. https://doi.org/10.4060/ca9692en. (Accessed: 11 Sept 2025).
13. Fosberg, F. R., 1958. Man as a dispersal agent. The Southwestern Naturalist, 3(1), 1–6.
14. Godfray, H. C. J., Beddington,J. R.,Crute,I. R.,Haddad, L., Lawrence, D., Muir, J.F.,Toulmin, C., 2010. Food security: The challenge of feeding 9 billion people. Science, 327(5967), 812–818. doi:10.1126/science.1185383.
15. Gökalp, Ö., Üremiş, İ., 2015. Mardin’de buğday ürününe karışan yabancı ot tohumlarının belirlenmesi. Mustafa Kemal Üniversitesi Ziraat Fakültesi Dergisi, 20(1), 23–30.
16. Güncan, A., 1980. Anadolu’nun Doğusunda Buğday Ürününe Karışan Yabancı Ot Tohumları, Bunların Yoğunlukları ve Önemlilerinin Oluşturdukları Bitki Toplulukları Üzerinde Bir Araştırma. Atatürk Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, 48s., Erzurum.
17. Güncan, A., 2001. Anadolu’ nun doğusunda buğday ürününe karışan yabancı ot tohumları, bunların yoğunlukları ve önemlilerinin oluşturdukları bitki toplulukları (Assosiation) üzerinde bir araştırma.Konya Ticaret Borsası Dergisi, Konya.
18. Güncan, A., 2006. Yabancı ot mücadelesi. Selçuk Üniversitesi Ziraat Fakültesi yayınları ISBN: 975-448-178-4, 2006, Konya.
19. Güncan, A., 2016. Yabancı Otlar ve Mücadele Prensipleri. S. Ü. Basımevi, 311 s. Konya.ISBN:978-975-448-157-0.
20. Gürbüz, R., Alma, M. H., Alptekin, H.,Tülek, C., 2024. Performance of Some Organic Mulch Materials for Weed Suppression, Soil Conditions and Yield in Capsicum annuum L. Cultivation. Journal of the Institute of Science and Technology, 14(1),18-38. https://doi.org/10.21597/jist.1326729.
21. Gürbüz, R., Alptekin, H., 2024. Effect of pre-and post-emergence herbicides on weed control and yield in sunflower (Helianthus annuus L.). Türkiye Tarımsal Araştırmalar Dergisi, 11(2), 141-156. https://doi.org/10.19159/tutad.1412074.
22. Hahn, J., Müller, J., Heiermann, M., 2025. Seed Survival in Silage: Reviewing 90 Years of Research. Plants, 14(3), 351. https://doi.org/10.3390/plants14030351.
23. Hakeem, S. A., Matloob, A., Ghazy, A. H., Fiaz, S., Ali, M., Haq, T. U., Ul Haq, M. Z., 2025. Critical period of weed-crop competition in irrigated chickpea as a tool for judicious weed management. Scientific Reports, 15(1), 32116.https://doi.org/10.1038/s41598-025-95013-x.
24. Haidar, M.A., Gharib, C., Sleiman,F.T., 2010. Survival of weed seeds subjected to sheep rumen digestion. Weed Research, 50(5), 467-471. https://doi.org/10.1111/j.1365-3180.2010.00797.x
25. Hendricks, S. B., Taylorson, R..B., 1976.Variation in germination and amino acid leakage of seeds with temperature related to membrane phase change. Plant Physiology, 58(1), 7-11. https://doi.org/10.1104/pp.58.1.7
26. Hosseini, P., Swett, C. L., Hanson, B. D., 2025. Addressing challenges of field equipment sanitation to reduce the spread of branched broomrape (Phelipanche ramosa) seed among California processing tomato fields. Weed Science, 73(1), e53. doi:10.1017/wsc.2025.10028.
27. Hulme, M., 2009. Why we disagree about climate change: understanding controversy, inaction and opportunity. Cambridge University Press, Cambridge.
28. Koç, E., Gürbüz. R., 2021.Tohumluk Üretim Aşamalarında Yabancı Ot Sorunu ve Çözüm Önerileri. Uluslararası Katılımlı Türkiye 7.Tohumculuk Kongresi. Iğdır, Türkiye. 15-17 Kasım 2021, Kitap özeti 62.sayfa.
29. Koch, W., Hurle, K., 1978. Grundlagen der Unkrautbekämpfung; UTB 513. Stuttgart, Ulmer, 207 S.
30. Michael, P. J.,Steadman,K. J.,Plummer, J. A.,Vercoe, P., 2006. Sheep rumen digestion and transmission of weedy Malva parviflora seeds. Australian Journal of Experimental Agriculture, 46(10), 1251-1256.
31. McKenzie-Gopsill,A., Nyiraneza, J., Fillmore, S., 2024.Effects of cultural practices on weed community and seedbank dynamics in a potato rotation.Field Crops Research, 310,109357.
32. Nikolić, N., Massaro, S., Tagliapietra, F., Schiavon, S., Masin, R., 2025. Post digestion weed seed survival in cattle. Frontiers in Plant Science, 16, 1483774. DOI: 10.3389/fpls.2025.1483774.
33. Oveisi, M., Ojaghi, A., Rahimian Mashhadi, H., Müller‐Schärer, H., Reza Yazdi, K., Pourmorad Kaleibar, B., Soltani, E., 2021. Potential for endozoochorous seed dispersal by sheep and goats: Risk of weed seed transport via animal faeces. Weed Research, 61(1), 1-12. https://doi.org/10.1111/wre.12461.
34. Owen, M. J., Powles, S. B., 2020. Lessons learnt:crop-seed cleaning reduces weed-seed contamination in Western Australian grain samples. Crop and Pasture Science, 71(7), 660-667. DOI: 10.1071/CP20093
35. Önen, H., Özaslan C., Çaldıran U., 2015. Persicaria perfoliata (L.) H. Gross. S: 410-423. Türkiyeİstilacı Bitkiler Kataloğu, Editör Huseyin ONEN, T.C. Gıda, Tar. ve Hay. Bakanlığı.Tagem, Bit. Sağ. Araş. Daire Başk., Ankara, ISBN: 978-605-9175-05-0.
36. Önen, H., 2015. İstilacı yabancı türler ve istila süreçleri. Türkiye istilacı bitkiler kataloğu, S: 1-13. Editör Huseyin Onen, T.C. Gıda, Tar. ve Hay. Bakanlığı. TAGEM, Bit. Sağ. Araş.Daire Başk. Türkiye, ISBN: 978-605-9175-05-0.
37. Önen, H., 2021. Yabancı Otlar ve Herboloji (Yabancı Ot Bilimi), 2. Bölüm. “Herboloji (Yabancı Ot Bilimi): İlkeler, Kavramlar ve Uygulamalar / Weed Science: Theory and Practice” içinde (s. 827). Adana,10.13140/RG.2.2.10113.99688.
38. Özer, Z., Kadıoğlu, İ., Önen, H., Tursun, N., 2001. Herboloji (Yabancı Ot Bilimi). Genişletilmiş 3. Baskı. Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Yayınları No: 20. Kitaplar SerisiNo:10. GOP. Üniversitesi Basımevi.Tokat.ISBN:975.7328.16.2.
39. Özkil, M., Kara, A., 2006. Trakya bölgesinde selektörden önce ve sonra buğday ürününe karışan yabancı ot tohumlarının belirlenmesi. Trakya Üniversitesi Fen Bilimleri Dergisi, 7(1), 45–52.
40. Piggin, C. M., 1978. Dispersal of Echium plantagineum L. by sheep. Weed Research, 18(3), 155-160.
41. Sade, B.A., Topal, S., Soylu, S., 1999. Araştırmaların ışığı altında Konya ekolojisinde buğday ve arpa yetiştirme tekniği. Orta Anadolu’da Hububat Tarımının Sorunları ve Çözüm Yolları Sempozyumu, 8–11 Haziran, Konya, Türkiye.
42. Sakai, S., Kikuzawa, K.,Umeki, K., 1998. Evolutionarily stable resource allocation for production of wind-dispersed seeds. Evolutionary Ecology, 12(4), 477-485.https://doi.org/10.1023/A:1006576906198.
43. Sırma, M., Kadıoğlu, İ., Güncan, A., 1997. Tokat yöresinde tohumluk buğdayda selektörden önce ve sonra ürüne karışan yabancı ot tohumlarının belirlenmesi. Türkiye II. Herboloji Kongresi, 279.
44. Singh, A., Smith,B., Russell, D., Price, A. J., McElroy, J. S.,Maity, A., 2025. Endozoochorous seed dispersal potential of five major southern US weeds. Weed Science, 73(1), e54.https://doi.org/10.1017/wsc.2025.10027.
45. Sırrı, M., 2023. Mercimek (Lens culinaris Medic.) Ürününe Karışan Yabancı Ot Tohumlarının Belirlenmesi. Türkiye Tarımsal Araştırmalar Dergisi, 10(2): 220-230. https://doi.org/10.19159/tutad.1309664.
46. Taşkın, R., Gürbüz, R., Alptekin, H., 2025. The Frequency and Densıty of Weeds in Peanut (Arachis hypogaea L.)Fıelds of Adana Provınce, Türkiye. Research in Agricultural Sciences, 56(2), 112-121. https://doi.org/10.17097/agricultureatauni.1613076.
47. Thompson, K., Grime, J. P., 1979. Seasonal variation in the seed banks of herbaceous species in ten contrasting habitats. The Journal of Ecology, 893-921. https://doi.org/10.2307/2259220.
48. Tilman, D., Balzer, C., Hill, J., Befort, B. L., 2011. Global food demand and the sustainable intensification of agriculture. Proceedings of the national academy of sciences, 108(50), 20260-20264.64. https://doi.org/10.1073/pnas.1116437108.
49. Traveset, A., 1998.Effect of seed passage through vertebrate frugivores' guts on germination:a review. Perspectives in Plant ecology, evolution and systematics, 1(2),151-190. https://doi.org/10.1078/1433-8319-00057.
50. Traveset, A., Heleno, R., Nogales, M., 2014.The ecology of seed dispersal. In Seeds: the ecology of regeneration in plant communities (pp. 62-93). Wallingford UK: CABI. https://doi.org/10.1079/9781780641836.0062
51. Tursun, N., Kantarcı, Z., Seyithanoğlu, M., 2004. Adıyaman ve Gaziantep bölgelerinde buğday ürününe karışan yabancı ot tohumlarının belirlenmesi. Türkiye Herboloji Dergisi, 7(1), 1–12.
52. Tülek, C., Gürbüz, R., Alptekin, H., 2022. Organik malç materyallerinin domates (Solanum lycopersicum L.)’te yabancı ot kontrolüne etkisi. Journal of Agriculture, 5(2), 86-101. https://doi.org/10.46876/ja.1208575.
53. Uruç, B., Bozdoğan, O., 2024. Diyarbakır İlinde Mercimek Ürününe Karışan Yabancı Ot Tohumlarının Belirlenmesi. Journal of the Institute of Science and Technology, 14(2), 563-593. https://doi.org/10.21597/jist.1386641
54. Vittoz, P., Engler, R., 2007. Seed dispersal distances: A typology based on dispersal modes and plant traits. Botanica Helvetica, 117, 109–124. doi:10.1007/s00035-007-0797-8.
55. Walsh, M. J., Powles, S. B., 2014. High seed retention at maturity of annual weeds infesting crop fields highlights the potential for harvest weed seed control. Weed Technology, 28(3), 486-493. doi:10.1614/WT-D-13-00183.1.
56. Walsh, M. J., Broster, J. C., Schwartz‐Lazaro, L. M., Norsworthy, J. K., Davis, A. S., Tidemann, B. D., Bagavathiannan, M.V., 2018. Opportunities and challenges for harvest weed seed control in global cropping systems. Pest Management Science, 74(10), 2235-2245.https://doi.org/10.1002/ps.4802
57. Wilson, C. E., Castro, K. L., Thurston, G. B., Sissons, A. 2016. Pathway risk analysis of weed seeds in imported grain: A Canadian perspective. NeoBiota, 30, 49-74. doi:10.3897/neobiota.30.7502.
58. Zengin, H., 1996. Erzurum ve ilçelerinde yazlık buğday ürününe karışan yabancı ot tohumları ve yoğunlukları üzerinde araştırmalar. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 27(3), 411–422.