IDENTIFICATION OF BACTERIA ISOLATED FROM WILD PLANTS TAKEN FROM DIFFERENT LOCATİONS AND DETERMINATION OF PROPERTIES OF NITROGEN FIXATION, PHOSPHORUS, POTASSIUM AND CALCİUM SOLUBILIZING

Abstract

In this study, 246 bacterial strains were isolated from 23 healthy plant samples were taken from different provinces. Bacterial strains were tested for hypersensitive response (HR) on tobacco. HR test showed that bacterial strains were not pathogenic. The strains were identified by using the Microbial Identification System. According to the fatty acid methyl ester analysis results bacterial strains were determined as follow; Arthbacter (17), Brevibacillus (12), Bacillus (65), Lysinibacillus (3), Herbaspirillum (7), Kocuria (21), Paucimonas (8), Pseudomonas (36), Virgibacillus (3), Microbacterium (11), Micrococcus (8), Erwinia (4), Stenotrophomonas (8), Nesterenkonia (1), Achromobacter (1), Curtobacterium (5), Rhodococcus (7), Enterobacter (2), Escherichia (1), Chryseobacterium (1), Xanthomonas (3), Acinetobacter (5), Rothia (1), Paenibacillus (1), Ochrobacterium (1), Pantoea (1), Sphingbacterium (5), Rhizobium (3), Grimontia (1), Aeromonas (1) , Brevundimonas (1), Phyllobacterium (1) ve Staphylococcus (1). The obtained bacterial strains were tested for nitrogen fixing, phosphate, potassium and calcium solubilising properties. Herbaspirillum huttiense (SK4, SK49), Microbacterium esteraromaticum (SK19, SK39, SY48), Achromobacter xylosoxidans (SK50), Paucimonas lemoignei (SK56), Pantoea agglomerans (SY43) , Pseudomonas putida biotype B (YS2, DT17), Pseudomonas syringae pv. syringae (EP19) and Pseudomonas pseudoalcaligenes (SA20) strains were found to be positive in all tests and the test results of other strains were found to be variable.

Keywords

• MIS
• Phosphate Solubilizing Bacteria
• Potassium Solubilizing Bacteria
• Calcium Solubilizing Bacteria
• Nitrogen Fixation

FARKLI LOKASYONLARDA YABANİ BİTKİ TÜRLERİNDEN İZOLE EDİLEN BAKTERİLERİN TANISI VE AZOT FİKSE ETME, FOSFOR, POTASYUM VE KALSİYUM ÇÖZME ÖZELLİKLERİNİN BELİRLENMESİ

Abstract

Bu çalışmada, farklı illerden alınan 23 sağlıklı bitki örneğinden yapılan izolasyon sonucunda 246 bakteri straini elde edilmiştir. Tütünde yapılan HR testi ile bakteri strainlerinin patojen olmadıkları belirlenmiştir. Strainler Mikrobial Tanı Sistemi kullanılarak yağ asit metil analizi ile Arthbacter (17), Brevibacillus (12), Bacillus (65), Lysinibacillus (3), Herbaspirillum (7), Kocuria (21), Paucimonas (8), Pseudomonas (36) , Virgibacillus (3), Microbacterium (11), Micrococcus (8), Erwinia (4), Stenotrophomonas (8), Nesterenkonia (1), Achromobacter (1), Curtobacterium (5), Rhodococcus (7), Enterobacter (2), Escherichia (1), Chryseobacterium (1), Xanthomonas (3), Acinetobacter (5), Rothia (1), Paenibacillus (1), Ochrobacterium (1), Pantoea (1), Sphingbacterium (5), Rhizobium (3), Grimontia (1), Aeromonas (1), Brevundimonas (1), Phyllobacterium (1) ve Staphylococcus (1) olarak belirlenmiştir. Elde edilen bakteri strainleri azot fiksasyonu, fosfat, potasyum ve kalsiyum çözücü özellikleri bakımından test edilmiştir. Bunlar arasında Herbaspirillum huttiense (SK4, SK49), Microbacterium esteraromaticum (SK19, SK39, SY48), Achromobacter xylosoxidans (SK50), Paucimonas lemoignei (SK56), Pantoea agglomerans (SY43), Pseudomonas putida biotype B (YS2, DT17), Pseudomonas syringae pv. syringae (EP19) ve Pseudomonas pseudoalcaligenes (SA20) olmak üzere 12 tane strainin bütün testlerde pozitif sonuç verdiği, diğer strainlerin test sonuçlarının ise değişkenlik gösterdiği belirlenmiştir.

Keywords

• MIS
• Fosfat Çözen Bakteri
• Potasyum Çözen Bakteri
• Kalsiyum Çözen Bakteri
• Azot Fiksasyonu

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