Biological Control of Rhizoctonia Solani Kühn. with Rhizobacteria Isolated from Different Soiland Calligonum Polygonoides L. Subsp. Comosum (L’hér.)

Year 2021, Volume: 4 Issue: 2, 92 - 107, 31.12.2021

Halit Anak , Mesude Figen Dönmez , İrfan Çoruh

https://doi.org/10.46876/ja.986625

Cited By: 1

Abstract

In this study, the biological control activities of the bacteria strains, which have been isolated from the volcanic, sandy, salty soils and Ebu Cehil plant Calligonum polygonoides L. subsp. comosum (L’Hér.) were investigated against isolates of Rhizoctonia solani causing root rot in beans. As the result of the isolation, 83 strains were obtained and the bacteria were identified by biochemical tests and fatty acid methyl esterase analyses. In the in vitro conditions, 20 strains from among 83 bacteria strains were found to be effective and it was determined that these strains inhibited the mycelial growth by forming a prevention zone ranging between 0.1 cm and 2.4 cm. The prevention rate of bacteria strains against pathogen isolates was found to range between 50%-66% maximum. In the in vivo conditions, the bacteria strains were found to successfully inhibit the disease development compared to the control group. Bacillus thuringiensis israelensis HV43, Bacillus subtilis HV34, Bacillus cereus GC subgroup A HT21 and Bacillus subtilis HT30 strains were determined to prevent the disease incidence with an effectiveness of 100%.

Keywords

Bean, Rhizoctonia solani, Biological control, Calligonum polygonoides (L.’Hér)

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