Pathogenicity of Beauveria bassiana (Balsamo) Vuillemin F7-1 on Sitophilus oryzae L. (Coleoptera: Curculionidae) Adults and the Effect of Ambient Humidity

Year 2024, Volume: 7 Issue: 1, 10 - 16, 30.06.2024

Zeynep Şahin Taylan , Mehmet Kubilay Er

https://doi.org/10.46876/ja.1402159

Abstract

Sitophilus oryzae causes considerable damage in stored grain. Considering sustainable agricultural practices biological control plays an important role in the control of this pest. In this study, the pathogenicity of a Beauveria bassiana isolate (F7-1) against S. oryzae adults and the effect of ambient relative humidity (RH) were investigated. For each experimental unit, fungal spores were applied to 40 gr wheat kernels within a 50 ml centrifuge tube. Thereafter, S. oryzae adults were placed in the tube. The tests were conducted at 26±2 °C temperature, 65±5% RH in darkness. The mortality of S. oryzae was recorded 7 and 14 later. For the pathogenicity test, spore concentrations were applied at 250, 500, 1000 and 1500 ppm. Increasing with the concentration, lethal effect reached to a maximum of 87.7%. According to the results of the experiment carried out to determine the effect of ambient relative humidity on fungal pathogenicity, adult mortality did not differ statistically at 65-75% RH. However, when RH was increased to 100%, the mortality reached to a statistically higher level of 91.6%. It is concluded that B. bassiana F7-1 has a considerable potential providing appropriate concentrations, and ambient humidity did not have a limiting effect except for extreme conditions.

Keywords

Rice weevil, Biological control, Microbial control, Hypocreales

References

• Abbott, W.S., 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 265-267.
• Acheampong, M.A., Coombes, C.A., Moore, S.D., Hill, M.P., 2020. Temperature tolerance and humidity requirements of select entomopathogenic fungal isolates for future use in citrus IPM programmes. Journal of Invertebrate Pathology, 174(4):107436.
• Athanassiou, C.G., Steenberg, T., 2007. Insecticidal effect of Beauveria bassiana (Balsamo) Vuillemin (Ascomycota: Hypocreales) in combination with three diatomaceous earth formulations against Sitophilus granarius (L.) (Coleoptera: Curculionidae). Biological Control, 40(3): 411–416.
• Batta, Y.A., 2003. Control of rice weevil (Sitophilus oryzae L., Coleoptera: Curculionidae) with various formulations of Metarhizium anisopliae. Crop Protection, 23(2):103–108.
• Batta, Y.A. and Kavallieratos, N.G., 2018. The use of entomopathogenic fungi for the control of stored-grain insects. International Journal of Pest Management. Volume 64(1): 77-78.
• Bello, S., Padin, C., Fabrizio, M., 2000. Laboratory evaluation of chemical-biological control of the rice weevil (Sitophilus oryzae L.) in stored grains. Journal of Stored Products Research, 37(1): 77–84.
• Bora, T., Ozaktan, H., 1998. Biological Control of Plant DiseaseS. Prizma Press, Izmir, 1. Edition, 205 pp.
• Demirci, F., Muştu, M., Kaydan, M.B., Ulgenturk, S., 2011. Laboratory evaluation of the effectiveness of the entomopathogen; Isaria farinosa, on citrus mealybug, Planococcus citri. Journal of Pest Science, 84: 337–342.
• Dizlek, H., 2012. The main factors that are effective upon the storage of cereals. Electronic Journal of Food Technologies, 7(2) 48-59
• FAO, 2023. Crops and livestock products. http://fao.org/faosttat/en/#data/QCL (Access 05.10.2023)
• Hagstrum, D.W., Flinn, P.W., 2014. Modern stored-product insect pest management. Journal of Plant Protection Research, 54(3):205-210.
• Kavallieratos, N.G., Athanassiou C.G., Aountala M.M, Kontodimas D.C., 2013. Evaluation of the entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea for control of Sitophilus oryzae. J Food Prot. 2014 Jan; 77(1):87-93.
• Lucas, E. and Riudavets, J., 2002. Biological and mechanical control of Sitophilus oryzae (Coleoptera: Curculionidae) in rice. Journal of Stored Products Research, 38(3):293-304.
• Mantzoukas, S., Kitsiou, F., Natsiopoulos. D., Eliopoulos, P.A., 2022. Entomopathogenic fungi: Interactions and applications. Encyclopedia, 2(2):646-656.
• Michalaki, M.P., Athanassiou, C.G., Steenberg, T., Buchelos, C.T., 2006. Effect of Paecilomyces fumosoroseus (Wise) Brown and Smith (Ascomycota: Hypocreales) alone or in combination with diatomaceous earth against Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae) and Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Biological Control, 40:280–286.
• Mishra, S., Kumar, P., & Malik, A., 2015. Effect of temperature and humidity on pathogenicity of native Beauveria bassiana isolate against Musca domestica L. Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology, 39(4): 697–704
• Ramaswamy, K. Vijayarangan, N. Krishnaiah, H.E., 2009. Insecticidal effect of Beauveria bassiana and Paecilomyces fumosoroseus alone and in combination with Diatomaceous earth against rice weevil (Sitophllus oryzae) and red flour beetle (Tribolium castaneum). Journal of Pure and Applied Microbiology, 3(2): 637-642.
• Rice, W.C. and Cogburn, R.R., 1999. Activity of the entomopathogenic fungus Beauveria bassiana (Deuteromycota: Hyphomycetes) against three coleopteran pests of stored grain. Stored Product and Quarantine Entomology. 92(3)-691-694
• SAS Institute., 2009. SAS/STAT User’s Guide, Version 9.1.3. Portable, SAS Institute, Cary, NC.
• Sewify, G.H., Shabrawy, H.A., Eweis M.E., Naroz, M.H., 2014. Efficacy of Entomopathogenic Fungi, Beauveria bassiana and Metarhizium anisopliae for Controlling Certain Stored Product Insects. Egyptian Journal of Biological Pest Control, 24(1):191-196.
• Sheeba, G., Seshadri, S., Nanja, N., Janarthanan, S., Ignacimuthu, S., 2000. Efficacy of Beauveria bassiana for control of the rice weevil Sitophilus oryzae (L.) (Coleoptera: Curculionidae). Applied Entomology and Zoology, 36(4):117-120.
• Sahin, Z., 2015. Effects of Beauveria bassiana isolates on Sitophilus oryzae adults under controlled conditions. Kahramanmaras Sutcu Imam University, Graduate School of Natural and Applied Sciences, Master Thesis (Published).
• Wakil, W., Riasat, T., Ghazanfar, M.U., Kwon, Y.J., Shaseen, F.A., 2011. Aptness of Beauveria bassiana and enhanced diatomaceous earth (DEBBM) for control of Rhyzopertha dominica. Entomological Research, 41:233–241.
• Vassilakos, T.N., Athanassiou, C.G., Kavallieratos, N.G., Vayias, B.J., 2006. Influence of temperature on the insecticidal effect of Beauveria bassiana in combination with diatomaceous earth against Rhyzopertha dominica and Sitophilus oryzae on stored wheat. Biological Control, 38:270–281
• Vincent, C., Panneton, B., Lessard, F.F., 2001. Physical control methods in plant protection. Springer-Verlag, Heidelberg, 253-256.
• Yildirim, E., 2000. Agricultural Pest Control Methods and Pesticides. Atatürk University Agriculture Faculty Course Publications. No: 219, Erzurum, 330 pp.
• Yildirim, E., Ozbek, H., Aslan, I., 2009. Stored Crop Pests. Atatürk University Agriculture Faculty Course Publications. No: 191, Erzurum, 122 pp.
• Zar, J.H., 1996. Biostatistical Analysis, 3rd Edition. Prentice Hall, Englewood Cliffs, NJ. Prentice-HaIl 620 pp.