Influence of Zeatin, Kinetin, and Gibberellic Acid Doses on Growth and Biochemical Responses of Melissa officinalis L. (Lemon Balm) Seedlings

Year 2024, Volume: 7 Issue: 2, 233 - 239, 31.12.2024

Muhammed Said Yolcu

https://doi.org/10.46876/ja.1577039

Abstract

This study aimed to assess the impact of Zeatin, Kinetin, and gibberellic acid biostimulant doses key regulators of plant growth and development on the growth and biochemical properties of Melissa officinalis (lemon balm). Conducted under greenhouse conditions using a Completely Randomized Experimental Design with three replicates, the research measured parameters such as seedling and root lengths, fresh and dry weights of seedlings and roots, total phenolic content, and antioxidant activities (CUPRAC and FRAP). The findings revealed that biostimulant treatments significantly improved growth parameters compared to the control, with the exception of root length. The 200 ppm gibberellic acid treatment yielded the highest fresh and dry weights for both seedlings and roots. For biochemical effects, the highest antioxidant activity was recorded in Zeatin40 treatments, while the greatest phenolic content was observed in Zeatin20, highlighting Zeatin’s effectiveness in enhancing total secondary metabolite content.

Keywords

Antioxidant, Biostimulant, Seedling Development, Lemon Balm, Total Phenolic Content

References

• Abdel-Naime, W.A., Fahim, J.R., Fouad, M.A., Kamel, M.S. (2019). Antibacterial, antifungal, and GC-MS studies of Melissa officinalis. S. Afr. J. Bot. 124, 228–234. https://doi.org/10.1016/j.sajb.2019.05.011
• Acidri, R., Sawai, Y., Sugimoto, Y., Handa, T., Sasagawa, D., Masunaga, T., ... & Nishihara, E. (2020). Exogenous kinetin promotes the nonenzymatic antioxidant system and photosynthetic activity of coffee (Coffea arabica L.) plants under cold stress conditions. Plants, 9(2), 281.
• Afroz, S., Mohammad, F., Hayat, S., Siddiqui, MH. 2005. Exogenous application of gibberellic acid counteracts the effect of sodium chloride in mustard. Turk. J. Biol., 29: 233–236.
• Aftab, T., Khan, M. M. A., Idrees, M., Naeem, M., Singh, M., Ram, M. 2010. Stimulation of crop productivity, photosynthesis and artemisinin production in Artemisia annua L. by triacontanol and gibberellic acid application. Journal of Plant Interactions, 5: 273–281.
• Ak, T., & Gülçin, I. (2008). Antioxidant and radical scavenging properties of curcumin. Chemico-biological interactions, 174(1), 27-37. http://dx.doi.org/10.1016/j.cbi.2008.05.003
• Akgül, A., (1993). Baharat Bilimi ve Teknolojisi. Gıda Teknolojisi Derneği Yayınları, 15, 111-113.
• Anonymous (2024) https://www.accuweather.com/tr/tr/arifbey/320558/november-weather/320558?year=2023. Access date: 17/09/2024.
• Baytop, T. (1984). Türkiye’de Bitkiler ile Tedavi: Geçmişte ve Bugün. İstanbul Üniversitesi, s/520
• Bhargava, A.; Clabaugh, I.; To, J.P.; Maxwell, B.B.; Chiang, Y.-H.; Schaller, G.E.; Loraine, A.; Kieber, J.J. Identification of Cytokinin-Responsive Genes Using Microarray Meta-Analysis and RNA-Seq in Arabidopsis. Plant Physiol. 2013, 162, 272–294.
• Brenner, W.G.; Schmulling, T. Transcript profiling of cytokinin action in Arabidopsis roots and shoots discovers largely similar but also organ-specific responses. BMC Plant Biol. 2012, 12, 112. [CrossRef] [PubMed]
• Ceylan, A. (1997). Tıbbi Bitkiler-II (Uçucu Yağ Bitkileri). Ege Üniversitesi Ziraat Fakültesi, s/188.
• Dadkhah, A., Ghorbanian, S., & Rassam, G. (2016). Foliar application effect of gibberelic acid on growth, volatile oil and nutrients content of Satureja hortensis L. Zeitschrift für Arznei-& Gewürzpflanzen, 21(2), 76-79.
• Dar, T. A., Uddin, M., Khan, M. M. A., Ali, A., Mir, S. R., & Varshney, L. (2015). Effect of Co-60 gamma irradiated chitosan and phosphorus fertilizer on growth, yield and trigonelline content of Trigonella foenum-graecum L. Journal of Radiation Research and Applied Sciences, 8(3), 446-458.
• Ghiulai, R., Avram, S., Stoian, D., Pavel, I.Z., Coricovac, D., Oprean, C., Vlase, L., Farcas, C., Mioc, M., Minda, D., et al. (2020). Lemon Balm Extracts Prevent Breast Cancer Progression In Vitro and In Ovo on Chorioallantoic Membrane Assay. Evid.-Based Complement. Altern. Med. 6489159. https://doi.org/10.1155/2020/6489159
• Gul, H., Khattak, A.M., Amin, N. 2006. Accelerat ing the growth of Araucaria heterophylla seedling through different gibberellic acid concentrations and nitrogen levels. J. Agric. Biol. Sci., 1: 25–29.
• Günaydın, M., Laghari, A. H., Bektaş, E., Sökmen, M., & Sökmen, A. (2017). Accumulation of phenolics in natural and micropropagated plantlets of Thymus pseudopulegioides Klokov & Des.-Shost. with their antioxidant potentials. Turkish Journal of Biology, 41(5), 754-764.
• Havlicek, L., Hanuš, J., Veselý, J., Leclerc, S., Meijer, L., Shaw, G. and Strnad, M. (1997). Cytokinin-derived cyclin-dependent kinase inhibitors: synthesis and cdc2 inhibitory activity of olomoucine and related compounds. Journal of medicinal chemistry, Volume 40, Issue 4, 408-412.
• Katar, D. (2004). Oğulotu (Melissa Officinalis L)’nda farklı bitki sıklığı ve azot dozlarının verim ve verim özelliklerine etkisi. Ankara Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi, Ankara, s.108.
• Katar, D., Gürbüz, B. (2008). Oğulotu (Melissa officinalis L.)’nda farklı bitki sıklığı ve azot dozlarının drog yaprak verimi ve bazı özellikler üzerine etkisi. Journal of Agricultural Sciences, 14(01), 78-81.
• Khan. M.N.. Khan. Z.. Luo. T.. Liu. J.H.. Rizwan. M.. Zhang. J.. Xu. Z.H.. Wu. H.H.. Hu. L. Y.. 2020. Seed priming with gibberellic acid and melatonin in rapeseed: Consequences for improving yield and seed quality under drought and non-stress conditions. Ind. Crops Prod. 156. 112850 https://doi.org/10.1016/j. indcrop.2020.112850.
• Kocsy, G.; Tari, I.; Vanková, R.; Zechmann, B.; Gulyás, Z.; Poór, P.; Galiba, G. Redox control of plant growth and development. Plant Sci. 2013, 211, 77–91. [CrossRef] [PubMed]
• Miraj, S., Rafieian-Kopaei, M., Kiani, S. (2017). Melissa officinalis L: A Review Study with an Antioxidant Prospective. J. Evid.-Based Integr. Med. 22, 385–394. https://doi.org/10.1177/2156587216663433
• Mok, D. W., and Mok, M. C. (2001). “Cytokinin metabolism and action”. Annual review of plant biology, Volume 52, Issue 1, 89-118.
• Morales-Payan, J. 2005. Growth of aromatic coleus (Coleus amboinicus Lour.) as affected by biostimulators. Proceedings of 33rd PGRSA Annual Meeting, pp 210-212.
• Ouzounidou, G., Giannakoula, A., Asfi, M., & Ilias, I. (2011). Differential responses of onion and garlic against plant growth regulators. Pak. J. Bot, 43(4), 2051-2057.
• Patrick, D.J. (2015). Plant biostimulants: definition, concept, main categories and regulation. Scientia Horticulture. 196, 3 - 14. https://doi.org/10.1016/j.scienta.2015.09.021
• Patrick, D.J. (2015). Plant biostimulants: definition, concept, main categories and regulation. Scientia Horticulture. 196, 3 - 14. https://doi.org/10.1016/j.scienta.2015.09.021
• Petrisor, G., Motelica, L., Craciun, L. N., Oprea, O. C., Ficai, D., & Ficai, A. (2022). Melissa officinalis: Composition, pharmacological effects and derived release systems—A review. International journal of molecular sciences, 23(7), 3591. https://doi.org/10.3390/ijms23073591
• Ravanfar, S. A., Karimi, E., Mehrabanjoubani, P., & Ebrahimi, M. (2020). Enhancement of phenolic and flavonoids compounds, antioxidant and cytotoxic effects in regenerated red cabbage by application of Zeatin. Natural product research, 34(6), 898-902.
• Reguera, M.; Peleg, Z.; Abdel-Tawab, Y.M.; Tumimbang, E.B.; Delatorre, C.A.; Blumwald, E. Stress-Induced Cytokinin Synthesis Increases Drought Tolerance through the Coordinated Regulation of Carbon and Nitrogen Assimilation in Rice. Plant Physiol. 2013, 163, 1609–1622.
• Sachett, A., Gallas-Lopes, M., Conterato, G. M. M., Herrmann, A. P., & Piato, A. (2021). Antioxidant activity by FRAP assay: in vitro protocol. Protocols, http://dx.doi.org/10.17504/protocols.io.btqrnmv6
• Santos, B., Morales-Payan, J.P, Stall, W.M., Dusky, J.A. 1998. Effects of nitrogen and gibberellic acid combination on basil growth. Soil Crop Sci.Soc. Florida Pro. 57: 99–110.
• Santos-Gomes, P. C., Seabra, R. M., Andrade, P. B., & Fernandes-Ferreira, M. (2003). Determination of phenolic antioxidant compounds produced by calli and cell suspensions of sage (Salvia officinalisL.). Journal of plant physiology, 160(9), 1025-1032.
• Shakeri, A., Sahebkar, A., Javadi, B. (2016). Melissa officinalis L.—A review of its traditional uses, phytochemistry and pharmacology. J. Ethnopharmacol. 188, 204–228. https://doi.org/10.1016/j.jep.2016.05.010
• Srivastava, N. K., & Srivastava, A. K. 2007. Influence of gibberellic acid on 14CO2 metabolism, growth, and production of alkaloids in Catharanthus roseus. Photosynthetica, 45: 156–160.
• Taiz, L., Zeiger, E. (2010). Plant Physiology (5th ed.) Sinauer Associates. Sunderland.
• Toprak, Ç. Ç. (2019). Bazı bitki büyüme düzenleyicilerinin dereotu (anethum graveolens l.)’nda bazı tarımsal özellikler ile uçucu yağ biyosentezi üzerine etkileri. Yüksek Lisans Tezi. Isparta.
• Waterhouse. A.L. Determination of total phenolics. Curr. Protoc. Food Anal. Chem. 2002. 6. I1.1.1–I1.1.8.
• Yousaf, M. J., Hussain, A., Hamayun, M., El-Sheikh, M. A., Elansary, H. O., & Kim, H. Y. (2024). Impact of cis-zeatin and lovastatin on antioxidant systems and growth parameters in Zea mays seedlings under phytohormonal crosstalks. Journal of Plant Interactions, 19(1), 2327378.
• Yousef, A.S.M., Gomma, A.O. 2008. Influence of GA3 application and Kristalon Fertilizer on growth, flowering and chemical composition of Dahlia pinnata plant (summer flowering type). Alex. J. Agric. Res. 53: 191–207.
• Yuan, L., Xu, D. Q. 2001. Stimulation effect of gibberellic acid short term treatment on leaf photosynthesis related to the increase in RuBis CO content in brood bean and soybean. Photo synthesis Research, 68: 39–47
• Zarei, A., Ashtiyani, S.C., Taheri, S., Rasekh, F. (20144). Comparison between effects of different doses of Melissa officinalis and atorvastatin on the activity of liver enzymes in hypercholesterolemia rats. Avicenna J. Phytomed. 4, 15–23. [CrossRef]
• Zeybek, N. (1987). İzmir’den ihraç edilen droglar, 59-64. V. Bitkisel İlaç Hammaddeleri Toplantısı, Ankara, s/408
• Zhu. J.. Wu. F.L.. Yue. S.N.. Chen. C.. Song. S.Q.. Wang. H.. Zhao. M.W.. 2019. Functions of reactive oxygen species in apoptosis and ganoderic acid biosynthesis in Ganoderma lucidum. Fems Microbiol. Lett. 366 (23). 23. https://doi.org/10.1093/ femsle/fnaa015.