EN
Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli
Abstract
During construction of genomic libraries from various bacteria, we experienced considerably different transformation effi ciencies among the strains of electrocompetent Escherichia coli cells and investigated the effect of type strain on effi ciency of transformation. Among the fi ve strains tested, Escherichia coli DH10B and TOP10 were found to be the most suitable strains for construction of genomic libraries in our laboratory. This study highlighted the fact that although these commercially available Escherichia coli strains are well characterized, they tend to differentiate and loose their transformation abilities. This may be due to the fact that the number of passages they have gone through affect their membrane structures and permeability. Therefore, every laboratory should test their strains for competency before they use them in their cloning studies
Keywords
Details
Primary Language
English
Subjects
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Journal Section
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Publication Date
July 1, 2008
Submission Date
July 1, 2008
Acceptance Date
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Published in Issue
Year 2008 Volume: 2 Number: 2
APA
Kasap, M., Torol, S., & Gacar, G. (2008). Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli. Journal of Applied Biological Sciences, 2(2), 19-22. https://izlik.org/JA99SD45ZU
AMA
1.Kasap M, Torol S, Gacar G. Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli. J.appl.biol.sci. 2008;2(2):19-22. https://izlik.org/JA99SD45ZU
Chicago
Kasap, Murat, Sinem Torol, and Gulcin Gacar. 2008. “Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia Coli”. Journal of Applied Biological Sciences 2 (2): 19-22. https://izlik.org/JA99SD45ZU.
EndNote
Kasap M, Torol S, Gacar G (July 1, 2008) Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli. Journal of Applied Biological Sciences 2 2 19–22.
IEEE
[1]M. Kasap, S. Torol, and G. Gacar, “Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli”, J.appl.biol.sci., vol. 2, no. 2, pp. 19–22, July 2008, [Online]. Available: https://izlik.org/JA99SD45ZU
ISNAD
Kasap, Murat - Torol, Sinem - Gacar, Gulcin. “Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia Coli”. Journal of Applied Biological Sciences 2/2 (July 1, 2008): 19-22. https://izlik.org/JA99SD45ZU.
JAMA
1.Kasap M, Torol S, Gacar G. Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli. J.appl.biol.sci. 2008;2:19–22.
MLA
Kasap, Murat, et al. “Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia Coli”. Journal of Applied Biological Sciences, vol. 2, no. 2, July 2008, pp. 19-22, https://izlik.org/JA99SD45ZU.
Vancouver
1.Murat Kasap, Sinem Torol, Gulcin Gacar. Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli. J.appl.biol.sci. [Internet]. 2008 Jul. 1;2(2):19-22. Available from: https://izlik.org/JA99SD45ZU