Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods

Musa Yavuz

EN

Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods

Abstract

In situ ruminal digestion procedure is known procedure for estimating ruminal digestion. In situ procedure requires correction for microbial contaminations, and the purine method has been used to correct microbial attachments. The neutral detergent fiber (NDF) method has been proposed as an alternative to the purine method. Objectives of this experiment were to compare purine and NDF method for microbial correction. Alfalfa hay, bermudagrass hay, tall fescue hay, corn and soybean meal samples were selected. Chemical analyses of dry matter (DM), crude protein (CP), NDF, Acid detergent fiber (ADF), NDF-protein (NDIP), ADF-protein (ADIP), lignin, crude oil and ash were determined on selected samples. In the experiment, samples were inserted into the rumen of a cannulated cow for 6, 12, 24, 72, and 96 h to determine in situ digestion. The average microbial purine to nitrogen ratio was determined to be 11.43. In situ digested tall fescue microbial protein corrected CP values with purine and NDF methods were similar within all hours, except 96 h. Other In situ digested sample purine procedure had some interference during sample reading and standard errors were not acceptable level. There were not enough data to compare both methods for other samples

Keywords

In situ ruminal digestion, bacterial protein, purine, neutral detergent fiber

Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Musa Yavuz This is me

Publication Date

July 1, 2009

Submission Date

July 1, 2009

Acceptance Date

-

Published in Issue

Year 2009 Volume: 3 Number: 2

IZ

https://izlik.org/JA32RT53DJ

Cite

RIS / Bibtex

APA

Yavuz, M. (2009). Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods. Journal of Applied Biological Sciences, 3(2), 1-5. https://izlik.org/JA32RT53DJ

AMA

1.Yavuz M. Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods. J.appl.biol.sci. 2009;3(2):1-5. https://izlik.org/JA32RT53DJ

Chicago

Yavuz, Musa. 2009. “Bacterial Protein Correction on In Situ Digested Samples With Purine or NDF Methods”. Journal of Applied Biological Sciences 3 (2): 1-5. https://izlik.org/JA32RT53DJ.

EndNote

Yavuz M (July 1, 2009) Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods. Journal of Applied Biological Sciences 3 2 1–5.

IEEE

[1]M. Yavuz, “Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods”, J.appl.biol.sci., vol. 3, no. 2, pp. 1–5, July 2009, [Online]. Available: https://izlik.org/JA32RT53DJ

ISNAD

Yavuz, Musa. “Bacterial Protein Correction on In Situ Digested Samples With Purine or NDF Methods”. Journal of Applied Biological Sciences 3/2 (July 1, 2009): 1-5. https://izlik.org/JA32RT53DJ.

JAMA

1.Yavuz M. Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods. J.appl.biol.sci. 2009;3:1–5.

MLA

Yavuz, Musa. “Bacterial Protein Correction on In Situ Digested Samples With Purine or NDF Methods”. Journal of Applied Biological Sciences, vol. 3, no. 2, July 2009, pp. 1-5, https://izlik.org/JA32RT53DJ.

Vancouver

1.Musa Yavuz. Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods. J.appl.biol.sci. [Internet]. 2009 Jul. 1;3(2):1-5. Available from: https://izlik.org/JA32RT53DJ