Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli

Volume: 3 Number: 3 November 1, 2009
  • - Sreevıdhya
  • Lawrance Anbu Rajan
  • Yeragudipati Rajalakshmı
  • Vetrivel Umashankar
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Journal of Applied Biological Sciences
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Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli

Abstract

Trehalose, a non-reducing disaccharide is a major compatible solute, maintains fluidity of membranes and protects the biological structure of the organisms under stress. In this study, trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) genes encoding for trehalose biosynthesis were PCR amplified from genomic DNA of Escherichia coli isolated from Rohu (Labeo rohita) collected from local fish market of Cochin, Kerala, India. The amplified genes were cloned and nucleotide sequences were determined. The sequencing results showed that (TPS) and (TPP) genes contain1,424 bp and 801 bp long ORF encoding 474 and 266 amino acids respectively. In silico sequence analysis revealed that the (TPS) and (TPP) sequences of E. coli were conserved in many eubacteria

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Primary Language

English

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Journal Section

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Authors

- Sreevıdhya This is me

Lawrance Anbu Rajan This is me

Yeragudipati Rajalakshmı This is me

Vetrivel Umashankar This is me

Publication Date

November 1, 2009

Submission Date

November 1, 2009

Acceptance Date

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Published in Issue

Year 2009 Volume: 3 Number: 3

IZ

https://izlik.org/JA38RC96KX

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RIS / Bibtex
APA
Sreevıdhya, -, Rajan, L. A., Rajalakshmı, Y., & Umashankar, V. (2009). Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli. Journal of Applied Biological Sciences, 3(3), 55-59. https://izlik.org/JA38RC96KX
AMA
1.Sreevıdhya, Rajan LA, Rajalakshmı Y, Umashankar V. Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli. J.appl.biol.sci. 2009;3(3):55-59. https://izlik.org/JA38RC96KX
Chicago
Sreevıdhya, -, Lawrance Anbu Rajan, Yeragudipati Rajalakshmı, and Vetrivel Umashankar. 2009. “Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia Coli”. Journal of Applied Biological Sciences 3 (3): 55-59. https://izlik.org/JA38RC96KX.
EndNote
Sreevıdhya -, Rajan LA, Rajalakshmı Y, Umashankar V (November 1, 2009) Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli. Journal of Applied Biological Sciences 3 3 55–59.
IEEE
[1]- Sreevıdhya, L. A. Rajan, Y. Rajalakshmı, and V. Umashankar, “Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli”, J.appl.biol.sci., vol. 3, no. 3, pp. 55–59, Nov. 2009, [Online]. Available: https://izlik.org/JA38RC96KX
ISNAD
Sreevıdhya, - - Rajan, Lawrance Anbu - Rajalakshmı, Yeragudipati - Umashankar, Vetrivel. “Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia Coli”. Journal of Applied Biological Sciences 3/3 (November 1, 2009): 55-59. https://izlik.org/JA38RC96KX.
JAMA
1.Sreevıdhya -, Rajan LA, Rajalakshmı Y, Umashankar V. Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli. J.appl.biol.sci. 2009;3:55–59.
MLA
Sreevıdhya, -, et al. “Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia Coli”. Journal of Applied Biological Sciences, vol. 3, no. 3, Nov. 2009, pp. 55-59, https://izlik.org/JA38RC96KX.
Vancouver
1.- Sreevıdhya, Lawrance Anbu Rajan, Yeragudipati Rajalakshmı, Vetrivel Umashankar. Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli. J.appl.biol.sci. [Internet]. 2009 Nov. 1;3(3):55-9. Available from: https://izlik.org/JA38RC96KX