Determination of Plant Traits of Dwarf Fresh Bean Lines with Ayşe Kadın’ Characteristics and Gene Loci for The Resistance to Anthracnose (Colletotrichum lindemuthianum ( Sacc. & Magnus ) Lambs. Scrib.) Disease

Abstract

In this study, single line (pure-line) selection method was applied on local populations of dwarf fresh beans with the characteristics of Aysekadın type which were collected from Çarsamba, Terme, Tekkeköy towns of Çarsamba plain and Ladik region in 2003. Observantion gardens were set up in 2005 and consecutively pre-yield, local yield and regional yield tests were conducted in the years starting 2005 to 2009. In these lines, phenological observations and morphological measurements were determined in accordance to UPOV criteria and rates of their yields were examined. As a result of all assesments, 7 lines (KO, TK15, T26, Ç31, TK7, TK1 and T23) were approved to comfort with our purpose, selected as candidates for registration and were also examined for resistance to anthracnose disease by means of STS and SCAR molecular markers closely linked to anthracnose resistance Co- genes. While, resistance gene loci markers for Co-2, Co-4, Co-42 genes were present in all of the 7 lines, Co-1 gene locus marker was observed in only one of the line (KO) and 5 lines were positive for the marker of Co-6/co-8 gene loci. Two of the lines T26 and TK15 were registered under the variety names of “Karabacak” and “Zeynebim” respectively and given certification for production permits in 2009

Keywords

• Anthracnose, breeding, STS and SCAR molecular markers, Phaseolus vulgaris, pure-line selection, UPOV criteria