Enhanced Production and Stability of Laccase Using Some Fungi on Different Lignocellulosic Materials

Abstract

The potentials of thirteen lignocellulosic wastes wheat bran, barley bran, cone of Pinus nigra, sawdust, corn bran, oat, rice bran, canola, dried tea, ground clover straw, sunflower stalk, soybean bagasse, dried distillers grains with soluble (DDGS) as solid substrates in respect of laccase production by the white-rot fungi Trametes versicolor (ATCC 200801), Phanerochaete chrysospsorium (ME 446) and Pleurotus sajor-caju under solid-state conditions were assessed. The highest laccase activity was detected when T. versicolor was grown on ground clover straw, showing a maximum value of 22.36 U ml-1. The high enzyme activity was observed to correspond with chemical composition of substrate evaluated in respect of holocellulose, lignin, carbon and nitrogen contents. In addition, FTIR analysis was performed to chemical changes of substrate before and after using in solid state culture. Furthermore, pH and thermal stability of enzyme obtained from T. versicolor ATCC200801 grown on clover straw were studied. Also, effects of different inhibitors on the same enzyme activity were investigated

Keywords

• Solid state fermentation, laccase, FTIR, white rot fungi, stability