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Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation

Year 2011, Volume: 5 Issue: 1, 55 - 58, 01.02.2011

Özge Karakaş Cüneyt Uçarlı Nermin Gözükırmızı Filiz Gürel

https://izlik.org/JA46WP25PK

Abstract

This study describes a protocol for Agrobacterium-mediated transformation of barley mature embryos. Cultured embryos were co-cultivated by EHA 105 strain carrying pCambia2301 binary vector and assayed for transient GUS expression. After 3 days of transformation, GUS expressing spots have been observed over 20.3% of total embryos. Among the tested several pre-treatments, infiltration in a vacuum chamber (400 mm Hg for 20 min) and infection by AGL-1 strain instead of EHA 105 increased the numbers of embryos with GUS staining significantly (p<0.05). PCR and Southern analyses showed that transformation occured in some of the T0 plants which were also tolerant to selective conditions

Keywords

Hordeum vulgare L. Agrobacterium-mediated transformation mature embryo

Year 2011, Volume: 5 Issue: 1, 55 - 58, 01.02.2011

Özge Karakaş Cüneyt Uçarlı Nermin Gözükırmızı Filiz Gürel

https://izlik.org/JA46WP25PK

Abstract

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Details

Other ID JA67VK79MR
Authors

Özge Karakaş This is me

Cüneyt Uçarlı This is me

Nermin Gözükırmızı This is me

Filiz Gürel This is me

Publication Date February 1, 2011
IZ https://izlik.org/JA46WP25PK
Published in Issue Year 2011 Volume: 5 Issue: 1

Cite

APA Karakaş, Ö., Uçarlı, C., Gözükırmızı, N., & Gürel, F. (2011). Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. Journal of Applied Biological Sciences, 5(1), 55-58. https://izlik.org/JA46WP25PK
AMA 1.Karakaş Ö, Uçarlı C, Gözükırmızı N, Gürel F. Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. J.appl.biol.sci. 2011;5(1):55-58. https://izlik.org/JA46WP25PK
Chicago Karakaş, Özge, Cüneyt Uçarlı, Nermin Gözükırmızı, and Filiz Gürel. 2011. “Assessment of Barley (Hordeum Vulgare L.) Mature Embryos for Agrobacterium-Mediated Transformation”. Journal of Applied Biological Sciences 5 (1): 55-58. https://izlik.org/JA46WP25PK.
EndNote Karakaş Ö, Uçarlı C, Gözükırmızı N, Gürel F (February 1, 2011) Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. Journal of Applied Biological Sciences 5 1 55–58.
IEEE [1]Ö. Karakaş, C. Uçarlı, N. Gözükırmızı, and F. Gürel, “Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation”, J.appl.biol.sci., vol. 5, no. 1, pp. 55–58, Feb. 2011, [Online]. Available: https://izlik.org/JA46WP25PK
ISNAD Karakaş, Özge - Uçarlı, Cüneyt - Gözükırmızı, Nermin - Gürel, Filiz. “Assessment of Barley (Hordeum Vulgare L.) Mature Embryos for Agrobacterium-Mediated Transformation”. Journal of Applied Biological Sciences 5/1 (February 1, 2011): 55-58. https://izlik.org/JA46WP25PK.
JAMA 1.Karakaş Ö, Uçarlı C, Gözükırmızı N, Gürel F. Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. J.appl.biol.sci. 2011;5:55–58.
MLA Karakaş, Özge, et al. “Assessment of Barley (Hordeum Vulgare L.) Mature Embryos for Agrobacterium-Mediated Transformation”. Journal of Applied Biological Sciences, vol. 5, no. 1, Feb. 2011, pp. 55-58, https://izlik.org/JA46WP25PK.
Vancouver 1.Özge Karakaş, Cüneyt Uçarlı, Nermin Gözükırmızı, Filiz Gürel. Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. J.appl.biol.sci. [Internet]. 2011 Feb. 1;5(1):55-8. Available from: https://izlik.org/JA46WP25PK