Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation

Volume: 5 Number: 1 February 1, 2011
  • Özge Karakaş
  • Cüneyt Uçarlı
  • Nermin Gözükırmızı
  • Filiz Gürel
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Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation

Abstract

This study describes a protocol for Agrobacterium-mediated transformation of barley mature embryos. Cultured embryos were co-cultivated by EHA 105 strain carrying pCambia2301 binary vector and assayed for transient GUS expression. After 3 days of transformation, GUS expressing spots have been observed over 20.3% of total embryos. Among the tested several pre-treatments, infiltration in a vacuum chamber (400 mm Hg for 20 min) and infection by AGL-1 strain instead of EHA 105 increased the numbers of embryos with GUS staining significantly (p<0.05). PCR and Southern analyses showed that transformation occured in some of the T0 plants which were also tolerant to selective conditions

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Primary Language

English

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Journal Section

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Authors

Özge Karakaş This is me

Cüneyt Uçarlı This is me

Nermin Gözükırmızı This is me

Filiz Gürel This is me

Publication Date

February 1, 2011

Submission Date

February 1, 2011

Acceptance Date

-

Published in Issue

Year 2011 Volume: 5 Number: 1

IZ

https://izlik.org/JA46WP25PK

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RIS / Bibtex
APA
Karakaş, Ö., Uçarlı, C., Gözükırmızı, N., & Gürel, F. (2011). Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. Journal of Applied Biological Sciences, 5(1), 55-58. https://izlik.org/JA46WP25PK
AMA
1.Karakaş Ö, Uçarlı C, Gözükırmızı N, Gürel F. Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. J.appl.biol.sci. 2011;5(1):55-58. https://izlik.org/JA46WP25PK
Chicago
Karakaş, Özge, Cüneyt Uçarlı, Nermin Gözükırmızı, and Filiz Gürel. 2011. “Assessment of Barley (Hordeum Vulgare L.) Mature Embryos for Agrobacterium-Mediated Transformation”. Journal of Applied Biological Sciences 5 (1): 55-58. https://izlik.org/JA46WP25PK.
EndNote
Karakaş Ö, Uçarlı C, Gözükırmızı N, Gürel F (February 1, 2011) Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. Journal of Applied Biological Sciences 5 1 55–58.
IEEE
[1]Ö. Karakaş, C. Uçarlı, N. Gözükırmızı, and F. Gürel, “Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation”, J.appl.biol.sci., vol. 5, no. 1, pp. 55–58, Feb. 2011, [Online]. Available: https://izlik.org/JA46WP25PK
ISNAD
Karakaş, Özge - Uçarlı, Cüneyt - Gözükırmızı, Nermin - Gürel, Filiz. “Assessment of Barley (Hordeum Vulgare L.) Mature Embryos for Agrobacterium-Mediated Transformation”. Journal of Applied Biological Sciences 5/1 (February 1, 2011): 55-58. https://izlik.org/JA46WP25PK.
JAMA
1.Karakaş Ö, Uçarlı C, Gözükırmızı N, Gürel F. Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. J.appl.biol.sci. 2011;5:55–58.
MLA
Karakaş, Özge, et al. “Assessment of Barley (Hordeum Vulgare L.) Mature Embryos for Agrobacterium-Mediated Transformation”. Journal of Applied Biological Sciences, vol. 5, no. 1, Feb. 2011, pp. 55-58, https://izlik.org/JA46WP25PK.
Vancouver
1.Özge Karakaş, Cüneyt Uçarlı, Nermin Gözükırmızı, Filiz Gürel. Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. J.appl.biol.sci. [Internet]. 2011 Feb. 1;5(1):55-8. Available from: https://izlik.org/JA46WP25PK