Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii

Volume: 5 Number: 1 February 1, 2011
  • Muthuvel Arumugam
  • Thangappan Ajıthkumar
  • Thangavel Balasubramanıan
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Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii

Abstract

Type V like collagens is widely distributed in marine invertebrates, particularly crustaceans and molluscs. The present study the collagen was prepared from the outer skin of L. uyii. The net yield of L. uyii acid soluble collagen estimated as 10.54% (ASC), subsequent digestion of residue with pepsin soluble collagen was yielded as 31.16% (PSC). SDS-PAGE analysis was revealed the chain composition of (α1)2α2 heterotrimer for ASC which similar to Japanese common squid as well as cuttle fish collagen. The ASC and PSC total protein was determined by Bradford method which contains 64.27µg/ml and 18.93µg/ml respectively. Fourier transforms infrared spectroscopy Analysis (FT-IR) of ASC and PSC is helpful in predication and confirmation of secondary structure of proteins. This report indicates that L uyii outer skin has potential to supplementing the skin of land vertebrates as a source of collagen

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Primary Language

English

Subjects

-

Journal Section

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Authors

Muthuvel Arumugam This is me

Thangappan Ajıthkumar This is me

Thangavel Balasubramanıan This is me

Publication Date

February 1, 2011

Submission Date

February 1, 2011

Acceptance Date

-

Published in Issue

Year 2011 Volume: 5 Number: 1

IZ

https://izlik.org/JA72EN94BW

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RIS / Bibtex
APA
Arumugam, M., Ajıthkumar, T., & Balasubramanıan, T. (2011). Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii. Journal of Applied Biological Sciences, 5(1), 7-10. https://izlik.org/JA72EN94BW
AMA
1.Arumugam M, Ajıthkumar T, Balasubramanıan T. Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii. J.appl.biol.sci. 2011;5(1):7-10. https://izlik.org/JA72EN94BW
Chicago
Arumugam, Muthuvel, Thangappan Ajıthkumar, and Thangavel Balasubramanıan. 2011. “Isolation and Characterization of Type V Collagen from Outer Skin Waste of Loligo Uyii”. Journal of Applied Biological Sciences 5 (1): 7-10. https://izlik.org/JA72EN94BW.
EndNote
Arumugam M, Ajıthkumar T, Balasubramanıan T (February 1, 2011) Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii. Journal of Applied Biological Sciences 5 1 7–10.
IEEE
[1]M. Arumugam, T. Ajıthkumar, and T. Balasubramanıan, “Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii”, J.appl.biol.sci., vol. 5, no. 1, pp. 7–10, Feb. 2011, [Online]. Available: https://izlik.org/JA72EN94BW
ISNAD
Arumugam, Muthuvel - Ajıthkumar, Thangappan - Balasubramanıan, Thangavel. “Isolation and Characterization of Type V Collagen from Outer Skin Waste of Loligo Uyii”. Journal of Applied Biological Sciences 5/1 (February 1, 2011): 7-10. https://izlik.org/JA72EN94BW.
JAMA
1.Arumugam M, Ajıthkumar T, Balasubramanıan T. Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii. J.appl.biol.sci. 2011;5:7–10.
MLA
Arumugam, Muthuvel, et al. “Isolation and Characterization of Type V Collagen from Outer Skin Waste of Loligo Uyii”. Journal of Applied Biological Sciences, vol. 5, no. 1, Feb. 2011, pp. 7-10, https://izlik.org/JA72EN94BW.
Vancouver
1.Muthuvel Arumugam, Thangappan Ajıthkumar, Thangavel Balasubramanıan. Isolation and Characterization of Type V collagen from outer skin waste of Loligo uyii. J.appl.biol.sci. [Internet]. 2011 Feb. 1;5(1):7-10. Available from: https://izlik.org/JA72EN94BW