Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants

Volume: 6 Number: 1 February 1, 2012
  • Wafaa Kamal El-Din Hegazy
  • Sanaa Elsayed Ahmed Tork
  • Mohamed Safwat Abdel-salam
Journal of Applied Biological Sciences Cover
Journal of Applied Biological Sciences
PDF Download
EN

Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants

Abstract

This work aimed to characterize recombinant strains, with a high production level of L-asparaginase, previously obtained by protoplast fusion between Bacillus subtilis and Bacillus cereus. The genetic relationship between fusants and their parents was determined using a simple PCRrestriction fragment length polymorphism (RFLP) of 16S rDNAs -based method, and by analyzing the specifi c L-asparaginase genes and protein patterns. Our results indicate that all fusants have morphological characteristics and specifi c L-asparaginase genes similar to the Bacillus cereus strain whereas protein pattern and 16S rDNAs -RFLP analysis show mixed characteristics from both parents. Different genetic relationships were found between each fusant and its parental strain. These results confi rm that protoplast fusion is an important technique in strain improvement. It was used to combine genes from different organisms for creating strains with desired properties

Keywords

Details

Primary Language

English

Subjects

-

Journal Section

-

Authors

Wafaa Kamal El-Din Hegazy This is me

Sanaa Elsayed Ahmed Tork This is me

Mohamed Safwat Abdel-salam This is me

Publication Date

February 1, 2012

Submission Date

February 1, 2012

Acceptance Date

-

Published in Issue

Year 2012 Volume: 6 Number: 1

IZ

https://izlik.org/JA59AN78PT

Cite

RIS / Bibtex
APA
Hegazy, W. K. E.-D., Tork, S. E. A., & Abdel-salam, M. S. (2012). Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants. Journal of Applied Biological Sciences, 6(1), 25-30. https://izlik.org/JA59AN78PT
AMA
1.Hegazy WKED, Tork SEA, Abdel-salam MS. Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants. J.appl.biol.sci. 2012;6(1):25-30. https://izlik.org/JA59AN78PT
Chicago
Hegazy, Wafaa Kamal El-Din, Sanaa Elsayed Ahmed Tork, and Mohamed Safwat Abdel-salam. 2012. “Molecular Characterization of Overproducing L-Asparaginase Recombinant Bacillus Fusants”. Journal of Applied Biological Sciences 6 (1): 25-30. https://izlik.org/JA59AN78PT.
EndNote
Hegazy WKE-D, Tork SEA, Abdel-salam MS (February 1, 2012) Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants. Journal of Applied Biological Sciences 6 1 25–30.
IEEE
[1]W. K. E.-D. Hegazy, S. E. A. Tork, and M. S. Abdel-salam, “Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants”, J.appl.biol.sci., vol. 6, no. 1, pp. 25–30, Feb. 2012, [Online]. Available: https://izlik.org/JA59AN78PT
ISNAD
Hegazy, Wafaa Kamal El-Din - Tork, Sanaa Elsayed Ahmed - Abdel-salam, Mohamed Safwat. “Molecular Characterization of Overproducing L-Asparaginase Recombinant Bacillus Fusants”. Journal of Applied Biological Sciences 6/1 (February 1, 2012): 25-30. https://izlik.org/JA59AN78PT.
JAMA
1.Hegazy WKE-D, Tork SEA, Abdel-salam MS. Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants. J.appl.biol.sci. 2012;6:25–30.
MLA
Hegazy, Wafaa Kamal El-Din, et al. “Molecular Characterization of Overproducing L-Asparaginase Recombinant Bacillus Fusants”. Journal of Applied Biological Sciences, vol. 6, no. 1, Feb. 2012, pp. 25-30, https://izlik.org/JA59AN78PT.
Vancouver
1.Wafaa Kamal El-Din Hegazy, Sanaa Elsayed Ahmed Tork, Mohamed Safwat Abdel-salam. Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants. J.appl.biol.sci. [Internet]. 2012 Feb. 1;6(1):25-30. Available from: https://izlik.org/JA59AN78PT