Expression Pattern of Human ADAMTS-2 (A disintegrin and metalloproteinase with thrombospondin motif, 2) in Different Cell Lines

Abstract

The ADAMTSs (a disintegrin and metalloproteinase with thrombospondin motifs) are a family of metalloproteases that are found in mammals and invertebrates. This family is known to influence development, angiogenesis, coagulation and progression of arthritis. ADAMTS2 plays an essential role in the processing of the fibrillar collagen precursors into mature collagen molecules by cleaving of the amino terminus of the type I, II, III and V procollagen molecules. Collagens are the most abundant and important components of the extracellular matrix. Mutations in the ADAMTS-2 gene cause Ehler Danlos Syndrome Type VIIC in humans. Expression analysis of the ADAMTS-2 in some human tissues have been investigated so far. There is restricted studies on the expression pattern of ADAMTS-2 gene in cell lines. In this study, ADAMTS-2 mRNA expression levels were determined by semiquantitative RT-PCR analysis in MG-63, Saos-2, DU-145, PC-3, HUVEC, HeLa, HL-60 and Isichawa cell lines. ADAMTS-2 protein was visualised in Saos-2 osteosarcoma cell model by immunofluorescence for the first time. This preliminary studies about ADAMTS-2 gene expression levels will be beneficial for further ADAMTS-2 gene regulation studies in different cancer models

Keywords

• MMP, ADAMTS-2, collagen, sqRT-PCR, Immunofluorescence