Isolation of Cold Shock Protein profilles from Pseudomonas sp. Strains and Analyses of SDS-PAGE

Abstract

In this research, Pseudomonas aeruginosa balcalı-1, Pseudomonas aeruginosa balcalı-2 which taken Çukurova Univercity Balcalı Hospital Laboratory for cold shock protein isolation and Pseudomonas aeruginosa ATCC 27853 strains were used. For cold shock protein synthesis had choosen 20, 10, 5 ºC and the temperature values selected for 60 and 480 minutes incubation were carried out. Cold shock proteins to determine the molecular weight, SDS-PAGE gel system was used. P. aeruginosa ATCC 27853 strain isolated in 1700 and 18130Da weight proteins, were synthesized more than other proteins. These two proteins have occuranced with the cold shock treatment. P. aeruginosa Balcalı 1 produced the protein in 77066Da cold shock protein at 20ºC at for 60minutes and 5, 10, 20ºC for 480 minutes incubation SDS-PAGE analysis showed that P. aeruginosa Balcalı 2 strains numbered two different protein bands in 22666and 21576 Dalton molecular weight. Strains used in the study of Pseudomonas balcalı-1 and balcalı-2 from Cold Shock response higher constituted than Pseudomonas ATCC 27853. As a conclusion, Pseudomonas have three different strains Cold shock treatment when isolated the most cold shock protein, 10 ºC at for 60 minutes incubation

Keywords

• Pseudomonas aeruginosa. ,Cold Shock Protein, SDS-PAGE