Investigation on Cryptosporidium spp. in water samples collected from River Melet in Ordu by Loop Mediated Isothermal Amplification (LAMP)

Abstract

Cryptosporidium spp. we re identified in w ater s amples c ollected f rom R iver Melet in O rdu P rovince b y Loop M ediated I sothermal Amplification (LAMP). The water samples are regularly taken every month from River Melet where mix the sea point (M-1), near solid waste storage a rea (M -2), Ordu-Giresun o n t he h ighway b ridge l ocation ( M-3), n ear th e c ity's in dustry ( M-4) a nd i n the c ity out ( M-5) fro m December, 2 010 t o N ovember, 2 011. 6 0 w ater s amples were f locculated b y Aluminum S ulfate a nd t hey were u sed f or t he c ounting o f Cryptosporidium sp. oocysts by Modified Acid-Fast (MAF). The highest number of samples were purified by sucrose-gradient and DNA were extracted for LAMP assay. The number of oocycsts decreased in the form of M-3, M-4, M-2, M-1 ve M-5, respectively in five stations of River Melet. The six water samples from per site, which is the highest numbers of oocysts by MAF, were analyzed by LAMP and they were found positive. Ordu has rich water resources, but, the availability of them have been reduced because of the waste waters discharge of river and sea directly without processing. P arasites m ove t o r ivers, l akes an d s treams b y rainfall i n t his ar ea. T hereby, the d rinking, a griculture a nd recreational use of water is important to identify potential risk factors for the protection of the public health

Keywords

• Cryptosporidium, LAMP, River Melet