High Frequency Plant Regeneration of Dwarf Hygro (Hygrophila polysperma [Roxb.] T. Anderson) on Liquid Culture

Abstract

Dwarf hygro (Hygrophila polysperma [Roxb.] T. Anderson) is one of the popular aquatic plant of European countries. It is well-known medicinal plant in Indian states of Karnataka and West Bengal, and has been the part of Ayurvedic Medicine. The study presents direct organogenesis from shoot tip and 1st nodal meristem explant on liquid MS medium supplemented with 0.10-0.80 mg/l BA. Cent percent shoot regeneration without callusing was obtained from both explants after 6 weeks of culture. Maximum number of 25.33 and 21.67 shoots were recorded from shoot tip explant and 1st nodal meristem respectively on 0.10 mg/l BA containing medium. Shoot tip explants were further sub-cultured for 8 weeks and 87.5-100.0 % shoot regeneration frequency with 11.02-15.91 more shoots per explants and 0.51-1.61 cm longer shoots were recorded. However, total number of shoots per explant ranged 26.02-36.91. In vitro regenerated shoots were successfully rooted on solidified MS rooting medium containing 1.0 mg/l IBA. Rooted plantlets were acclimatized in aquariums containing tap water and sand where all plants survived

Keywords

• Dwarf hygro, in vitro, liquid medium, nodal meristem, shoot tip