EN
Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes
Abstract
Six different new Kluyveromyces lactis strains were isolated from different dairy products from Bursa province, Turkey. Lactase and invertase enzyme activities of new K. lactis strains were determined under different growth conditions. Our results indicate that both lactase and invertase enzyme activities of new K. lactis strains are much higher than the standard K. lactis (ATCC8585). Lactase and invertase enzymes are expressed at high levels under glucose repressed growth conditions in some of these K. lactis strains. Invertase activities of new K. lactis strains are 10-fold higher than the standard K. lactis (ATCC8585) strain. Additionally, it was shown that the two of the new K. lactis strains have 25% shorter duplication times than the normal K. lactis (ATCC8585). Our results indicate that new K. lactis strains isolated from the local dairy products of the Bursa region of Turkey have distinct metabolic features with respect to the regulation of GAL/LAC regulon of K. lactis
Keywords
Details
Primary Language
English
Subjects
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Journal Section
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Publication Date
November 1, 2013
Submission Date
November 1, 2013
Acceptance Date
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Published in Issue
Year 2013 Volume: 7 Number: 3
APA
Türkel, S., & Kayakent, N. (2013). Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes. Journal of Applied Biological Sciences, 7(3), 51-55. https://izlik.org/JA46FN88PM
AMA
1.Türkel S, Kayakent N. Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes. J.appl.biol.sci. 2013;7(3):51-55. https://izlik.org/JA46FN88PM
Chicago
Türkel, Sezai, and Nükhet Kayakent. 2013. “Isolation and Molecular Identification of New Kluyveromyces Lactis Strains Producing High Levels of Lactase and Invertase Enzymes”. Journal of Applied Biological Sciences 7 (3): 51-55. https://izlik.org/JA46FN88PM.
EndNote
Türkel S, Kayakent N (November 1, 2013) Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes. Journal of Applied Biological Sciences 7 3 51–55.
IEEE
[1]S. Türkel and N. Kayakent, “Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes”, J.appl.biol.sci., vol. 7, no. 3, pp. 51–55, Nov. 2013, [Online]. Available: https://izlik.org/JA46FN88PM
ISNAD
Türkel, Sezai - Kayakent, Nükhet. “Isolation and Molecular Identification of New Kluyveromyces Lactis Strains Producing High Levels of Lactase and Invertase Enzymes”. Journal of Applied Biological Sciences 7/3 (November 1, 2013): 51-55. https://izlik.org/JA46FN88PM.
JAMA
1.Türkel S, Kayakent N. Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes. J.appl.biol.sci. 2013;7:51–55.
MLA
Türkel, Sezai, and Nükhet Kayakent. “Isolation and Molecular Identification of New Kluyveromyces Lactis Strains Producing High Levels of Lactase and Invertase Enzymes”. Journal of Applied Biological Sciences, vol. 7, no. 3, Nov. 2013, pp. 51-55, https://izlik.org/JA46FN88PM.
Vancouver
1.Sezai Türkel, Nükhet Kayakent. Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes. J.appl.biol.sci. [Internet]. 2013 Nov. 1;7(3):51-5. Available from: https://izlik.org/JA46FN88PM