Synergistic Antifungal Effects of Quince Leaf’s Extracts and Silver Nanoparticles on Aspergillus niger

Abstract

Invasive fungal disease represents a major threat to life in immunocompromised patients and is now one of the most common causes of infection in this group. There is a limited range of antifungal agents available to treat disease caused by Aspergillus including the polyenes, flucytosine, azoles and more recently the echinocandins. Therefore it is necessary to find new ways of treatment for Aspergillus niger. The aim of this study was to investigate the synergistic antifungal effects of Quince leaf’s extracts and silver nanoparticles on A.niger in vitro. The ethanolic and acetonic extracts of Quince (Cydonia oblonga) leaf’s and silver nanoparticles prepared. Antifungal effects of extracts and silver nanoparticles against A. niger investigated by agar dilution method. Then minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) determined by the broth macro dilution method.Then synergistic effect was studied between the most effective plant extract with silver nanoparticles. The results showed that the C. oblonga extracts and silver nanoparticles can inhibit the growth of A. niger. Ethanolic extract was more effective extract that has antifungal activity against A. niger and synergistic activity observed between ethanolic extract of Quince’s leaf and silver nanoparticles against A. niger. Ethanolic extract of Quince’s leaf and silver nanoparticles has synergistic antifungal effect against A. niger and can be effective in controlling of A. niger

Keywords

• Antifungal Effect, synergism, Aspergillus niger, Cydonia oblonga, silver nanoparticles.