EN
Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp
Abstract
A cold-adaptive acidic cellulase enzyme was produced by native Bacillus sp. TG-1 from Adana-TURKEY and the cellulase producing bacteria and the enzyme was characterized. In SDS-PAGE analysis the enzyme showed single bond of 52.4 kDa. The cellulase TG-1 had a pH optimum of 4.6 and displayed maximum activity at 45ºC. The enzyme was stable at pH ranging from 4.0 to 9.0 with more than 85% of original activity for 24 h. Also, the enzyme showed 100% stability at 20-40ºC for 60 min and exhibited more than 63% of maximum activity in different NaCl concentrations. The enzyme showed up to 70% stability against detergents and chelators, while it was strongly inhibited by Urea, H2O2, PMSF and 1,10-Phenanthroline and induced by β-mercaptoethanol and MnCl2. TLC analyses of the hydrolysis of Carboxymethyl cellulose (CMC) by the enzyme, revealed the presence of maltose and etc
Keywords
Details
Primary Language
English
Subjects
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Journal Section
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Publication Date
November 1, 2014
Submission Date
November 1, 2014
Acceptance Date
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Published in Issue
Year 2014 Volume: 8 Number: 3
APA
Valıpour, E., Caf, Y., & Arıkan, B. (2014). Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp. Journal of Applied Biological Sciences, 8(3), 1-5. https://izlik.org/JA29JT32PR
AMA
1.Valıpour E, Caf Y, Arıkan B. Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp. J.appl.biol.sci. 2014;8(3):1-5. https://izlik.org/JA29JT32PR
Chicago
Valıpour, Ebrahim, Yasemin Caf, and Burhan Arıkan. 2014. “Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-Adaptive Cellulase (CMCase) from a Native Isolate Bacillus Sp”. Journal of Applied Biological Sciences 8 (3): 1-5. https://izlik.org/JA29JT32PR.
EndNote
Valıpour E, Caf Y, Arıkan B (November 1, 2014) Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp. Journal of Applied Biological Sciences 8 3 1–5.
IEEE
[1]E. Valıpour, Y. Caf, and B. Arıkan, “Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp”, J.appl.biol.sci., vol. 8, no. 3, pp. 1–5, Nov. 2014, [Online]. Available: https://izlik.org/JA29JT32PR
ISNAD
Valıpour, Ebrahim - Caf, Yasemin - Arıkan, Burhan. “Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-Adaptive Cellulase (CMCase) from a Native Isolate Bacillus Sp”. Journal of Applied Biological Sciences 8/3 (November 1, 2014): 1-5. https://izlik.org/JA29JT32PR.
JAMA
1.Valıpour E, Caf Y, Arıkan B. Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp. J.appl.biol.sci. 2014;8:1–5.
MLA
Valıpour, Ebrahim, et al. “Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-Adaptive Cellulase (CMCase) from a Native Isolate Bacillus Sp”. Journal of Applied Biological Sciences, vol. 8, no. 3, Nov. 2014, pp. 1-5, https://izlik.org/JA29JT32PR.
Vancouver
1.Ebrahim Valıpour, Yasemin Caf, Burhan Arıkan. Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp. J.appl.biol.sci. [Internet]. 2014 Nov. 1;8(3):1-5. Available from: https://izlik.org/JA29JT32PR