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Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp

Year 2014, Volume: 8 Issue: 3, 1 - 5, 01.11.2014

Ebrahim Valıpour Yasemin Caf Burhan Arıkan

Abstract

A cold-adaptive acidic cellulase enzyme was produced by native Bacillus sp. TG-1 from Adana-TURKEY and the cellulase producing bacteria and the enzyme was characterized. In SDS-PAGE analysis the enzyme showed single bond of 52.4 kDa. The cellulase TG-1 had a pH optimum of 4.6 and displayed maximum activity at 45ºC. The enzyme was stable at pH ranging from 4.0 to 9.0 with more than 85% of original activity for 24 h. Also, the enzyme showed 100% stability at 20-40ºC for 60 min and exhibited more than 63% of maximum activity in different NaCl concentrations. The enzyme showed up to 70% stability against detergents and chelators, while it was strongly inhibited by Urea, H2O2, PMSF and 1,10-Phenanthroline and induced by β-mercaptoethanol and MnCl2. TLC analyses of the hydrolysis of Carboxymethyl cellulose (CMC) by the enzyme, revealed the presence of maltose and etc

Keywords

Bacillus sp Cold–adaptive cellulase detergent energy saving

Year 2014, Volume: 8 Issue: 3, 1 - 5, 01.11.2014

Ebrahim Valıpour Yasemin Caf Burhan Arıkan

Abstract

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Details

Other ID JA83VN84HV
Authors

Ebrahim Valıpour This is me

Yasemin Caf This is me

Burhan Arıkan This is me

Publication Date November 1, 2014
Published in Issue Year 2014 Volume: 8 Issue: 3

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APA Valıpour, E., Caf, Y., & Arıkan, B. (2014). Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp. Journal of Applied Biological Sciences, 8(3), 1-5.
AMA Valıpour E, Caf Y, Arıkan B. Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp. J.appl.biol.sci. November 2014;8(3):1-5.
Chicago Valıpour, Ebrahim, Yasemin Caf, and Burhan Arıkan. “Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-Adaptive Cellulase (CMCase) from a Native Isolate Bacillus Sp”. Journal of Applied Biological Sciences 8, no. 3 (November 2014): 1-5.
EndNote Valıpour E, Caf Y, Arıkan B (November 1, 2014) Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp. Journal of Applied Biological Sciences 8 3 1–5.
IEEE E. Valıpour, Y. Caf, and B. Arıkan, “Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp”, J.appl.biol.sci., vol. 8, no. 3, pp. 1–5, 2014.
ISNAD Valıpour, Ebrahim et al. “Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-Adaptive Cellulase (CMCase) from a Native Isolate Bacillus Sp”. Journal of Applied Biological Sciences 8/3 (November2014), 1-5.
JAMA Valıpour E, Caf Y, Arıkan B. Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp. J.appl.biol.sci. 2014;8:1–5.
MLA Valıpour, Ebrahim et al. “Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-Adaptive Cellulase (CMCase) from a Native Isolate Bacillus Sp”. Journal of Applied Biological Sciences, vol. 8, no. 3, 2014, pp. 1-5.
Vancouver Valıpour E, Caf Y, Arıkan B. Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp. J.appl.biol.sci. 2014;8(3):1-5.