Microbial Potential of Lipase Production from Different Industrial Effluents

Abstract

Lipases hold significant position in the field of biotechnology due to its ability to catalyze esterification, interesterification, and transesterification reaction in nonaqueous media. In the present study four different lipase producing bacteria were isolated from effluents of leather, marble and textile industry. All the strains (L9, L15, L16 and L20) isolated were gram negative and subjected to both biochemical and molecular characterization. BLAST results for 16S rDNA sequences revealed that L9 (KJ748674) strain had 99% similarity with Acinetobacter lwoffi. L15 (KJ748675) and L16 (KJ864927) strains showed 98% and 99% sequence homology with Acinetobacter junni and strain L20 (KJ873872) belongs to Alishewanella agri sp. For enzyme production studies, pH and temperature were optimized on two different enrichment media. Comparison of two different enrichment media showed that medium containing peptone, yeast extract, NaCl and olive oil gave optimum production for all the strains. Alishewanella agri L20 (isolated from textile industry effluent) had growth at all temperatures (30, 37, 42 and 45°C) in medium containing yeast extract, NaCl and olive oil. In comparison to all the reported strains of the present study, Alishewanella agri (L20) had maximum enzyme production of 17 U/ml at elevated temperature of 45°C. Partial purification and SDS-PAGE analysis showed that protein bands of lipases were ranged between 35- 66.2 kDa. It is evident that high cost of lipase has generally reduced its exploitation at industrial level. Therefore, screening and identification of these lipolytic strains will act as a significant addition to the database on lipase research and its application at industrial level

Keywords

• Lipase, Industrial effluents, Acinetobacter junni, Acinetobacter lwoffi, Alishewanella agri, SDS-PAGE