The Molecular Detection of Lumpy Skin Disease Virus from Infected Cattle in Turkey

Abstract

LSD is endemic in Africa but through the last years disease begins to spread to the other countries. In Turkey, the disease has been detected for the first time in 2013 in East Mediterranean Province and then continued to spread and caused enormous economic losses through the other regions of country. In this study, we examined blood (n=1), skin nodules (n=2) from three cows suspected of LSD and conjunctival swab samples (n=1) from cow with conjunctivitis and corneal opasity (suspected of MCF) that were administered to our laboratory from four different dairy cattle herds between 2014 to 2015. By performing PCR and using specific primers for viral attachment protein and as a result, we have demonstrated that all the mentioned animals are positive. According to the our data, LSD could been seen in animals with different clinical conditions located in different regions of our country, and immediate transportation of LSDV suspicious samples to the efficient laboratories and correct diagnosis of the disease is an important factor for prevention of spreading, control (like quarantine/vaccination) of the disease and avoiding serious economical losses

Keywords

• Cattle, Conjuctivitis, Lumpy skin disease, PCR