Optimization of Tyrosinase Enzyme Production from Native Bacillus sp. MV29 Isolate

Abstract

Tyrosinase is a copper-containing enzyme which catalyzes the conversion of L-tyrosine to L-DOPA and melanin. L-DOPA is a preferred drug for treatment of Parkinson's disease and melanin has many pharmaceutically and therapeutically uses. In this research a native Bacillus sp. having tyrosinase enzyme was isolated from soil sample and the best carbon and nitrogen source for the enzyme production was found by analyzing effect of various carbon and nitrogen sources on the yield, also the optimum amount of the sources was selected by testing various amount of them. Various amounts of L-tyrosine and trace element were studied and the optimum amount of them was opted. Enzyme production by the native bacillus sp. was analyzed at different pH and temperature and the optimum pH value and temperature for the enzyme production was selected. By testing the enzyme activity for 56 hour at 4 hours intervals, the optimum incubation time was determined as 72 hour. According to the findings of this research, 40ᴼC as optimum temperature, pH 7.0 as optimum pH, 48h as optimum incubation time was determined as optimum for the enzyme production. The addition of L-tyrosine (5mM) as a substrate to the production medium was highly effective on enzyme production. Consequently, after optimization, 0.7IU tyrosinase enzyme per milliliter of medium culture was obtained

Keywords

• L-DOPA, melanin, Parkinson, Tyrosinase, optimization